All Outputs (43)

Developing genetic tools to control the Oriental fruit fly: Potential approaches and target genes (2024)
Journal Article
Traoré, N., Galizi, R., Kientega, M., Maïga, H., Nébié, K., Dabiré, R. A., …Diabaté, A. (in press). Developing genetic tools to control the Oriental fruit fly: Potential approaches and target genes. Journal of Applied Entomology, https://doi.org/10.1111/jen.13314

The Oriental fruit fly, Bactrocera dorsalis, is a pest of significant economic interest that damages horticulture and causes major economic losses worldwide. The main control measures are based on insecticides, which are being increasingly restricted... Read More about Developing genetic tools to control the Oriental fruit fly: Potential approaches and target genes.

Genetic Diversity and Population Structure of the Invasive Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae) in Burkina Faso (2024)
Journal Article
Traoré, N., Kientega, M., Maïga, H., Nebié, K., Zida, I., Galizi, R., …Diabaté, A. (2024). Genetic Diversity and Population Structure of the Invasive Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae) in Burkina Faso. Insects, 15(5), Article 298. https://doi.org/10.3390/insects15050298

Bactrocera dorsalis Hendel is a highly invasive horticultural pest that is of major economic importance worldwide. In Burkina Faso, it is one of the main insect pests that affects the production and exportation of mangos. Understanding the biology an... Read More about Genetic Diversity and Population Structure of the Invasive Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae) in Burkina Faso.

Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages (2024)
Journal Article
D’Amato, R., Taxiarchi, C., Galardini, M., Trusso, A., Minuz, R. L., Grilli, S., …Müller, R. (2024). Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages. Nature Communications, 15(1), Article 952. https://doi.org/10.1038/s41467-024-44907-x

CRISPR-based gene drives have the potential to spread within populations and are considered as promising vector control tools. A doublesex-targeting gene drive was able to suppress laboratory Anopheles mosquito populations in small and large cages, a... Read More about Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages.

Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics (2024)
Journal Article
Nayeem Hasan, M., Khalil, I., Baker Chowdhury, M. A., Rahman, M., Asaduzzaman, M., Billah, M., …Haider, N. (in press). Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics. GreSIS - Greater South Information System, https://doi.org/10.60692/x6t6v-mbt18

The objectives of this study were to compare dengue virus (DENV) cases, deaths, case-fatality ratio [CFR], and meteorological parameters between the first and the recent decades of this century (2000-2010 vs. 2011-2022) and to describe the trends, se... Read More about Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics.

Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics (2024)
Journal Article
Nayeem Hasan, M., Khalil, I., Baker Chowdhury, M. A., Rahman, M., Asaduzzaman, M., Billah, M., …Haider, N. (in press). Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics. GreSIS - Greater South Information System, https://doi.org/10.60692/gc5zx-3tc77

The objectives of this study were to compare dengue virus (DENV) cases, deaths, case-fatality ratio [CFR], and meteorological parameters between the first and the recent decades of this century (2000-2010 vs. 2011-2022) and to describe the trends, se... Read More about Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics.

Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics (2024)
Journal Article
Hasan, M. N., Khalil, I., Chowdhury, M. A. B., Rahman, M., Asaduzzaman, M., Billah, M., …Haider, N. (in press). Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics. Journal of Medical Entomology, Article tjae001. https://doi.org/10.1093/jme/tjae001

The objectives of this study were to compare dengue virus (DENV) cases, deaths, case-fatality ratio [CFR], and meteorological parameters between the first and the recent decades of this century (2000–2010 vs. 2011–2022) and to describe the trends, se... Read More about Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics.

Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome (2023)
Journal Article
Page, N., Taxiarchi, C., Tonge, D., Kuburic, J., Chesters, E., Kriezis, A., …Galizi, R. (2023). Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome. Communications Biology, 6(1), Article 850. https://doi.org/10.1038/s42003-023-05224-z

Understanding development and genetic regulation in the Anopheles gambiae germline is essential to engineer effective genetic control strategies targeting this malaria mosquito vector. These include targeting the germline to induce sterility or using... Read More about Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome.

Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome. (2023)
Journal Article
Page, N., Taxiarchi, C., Tonge, D., Kuburic, J., Chesters, E., Kriezis, A., …Galizi, R. (in press). Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome. Communications Biology, 6, Article 850. https://doi.org/10.1038/s42003-023-05224-z

Understanding development and genetic regulation in the Anopheles gambiae germline is essential to engineer effective genetic control strategies targeting this malaria mosquito vector. These include targeting the germline to induce sterility or using... Read More about Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome..

qSanger: Quantification of Genetic Variants in Bacterial Cultures by Sanger Sequencing (2023)
Journal Article
Prakash, S., Racovita, A., Petrucci, T., Galizi, R., & Jaramillo, A. (in press). qSanger: Quantification of Genetic Variants in Bacterial Cultures by Sanger Sequencing. BioDesign Research, 5, Article 0007. https://doi.org/10.34133/bdr.0007

Genetic variations such as mutations and recombinations arise spontaneously in all cultured organisms. Although it is possible to identify nonneutral mutations by selection or counterselection, the identification of neutral mutations in a heterogeneo... Read More about qSanger: Quantification of Genetic Variants in Bacterial Cultures by Sanger Sequencing.

The Potential for a Released Autosomal X-Shredder Becoming a Driving-Y Chromosome and Invasively Suppressing Wild Populations of Malaria Mosquitoes (2021)
Journal Article
Alcalay, Y., Fuchs, S., Galizi, R., Bernardini, F., Elaine Haghighat-Khah, R., Rusch, D. B., …Aris Papathanos, P. (2021). The Potential for a Released Autosomal X-Shredder Becoming a Driving-Y Chromosome and Invasively Suppressing Wild Populations of Malaria Mosquitoes. Frontiers in Bioengineering and Biotechnology, https://doi.org/10.3389/fbioe.2021.752253

Sex-ratio distorters based on X-chromosome shredding are more efficient than sterile male releases for population suppression. X-shredding is a form of sex distortion that skews spermatogenesis of XY males towards the preferential transmission of Y-b... Read More about The Potential for a Released Autosomal X-Shredder Becoming a Driving-Y Chromosome and Invasively Suppressing Wild Populations of Malaria Mosquitoes.

Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation (2021)
Journal Article
Fuchs, S., T. Garrood, W., Beber, A., Hammond, A., Galizi, R., Gribble, M., …Nolan, T. (2021). Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation. PLOS genetics, Article e1009740. https://doi.org/10.1371/journal.pgen.1009740

CRISPR-based homing gene drives can be designed to disrupt essential genes whilst biasing their own inheritance, leading to suppression of mosquito populations in the laboratory. This class of gene drives relies on CRISPR-Cas9 cleavage of a target se... Read More about Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation.

A genetically encoded anti-CRISPR protein constrains gene drive spread and prevents population suppression (2021)
Journal Article
Taxiarchi, C., Beaghton, A., Illansinhage Don, N., Kyrou, K., Gribble, M., Shittu, D., …Crisanti, A. (2021). A genetically encoded anti-CRISPR protein constrains gene drive spread and prevents population suppression. Nature communications, 1-8. https://doi.org/10.1038/s41467-021-24214-5

CRISPR-based gene drives offer promising means to reduce the burden of pests and vector-borne diseases. These techniques consist of releasing genetically modified organisms carrying CRISPR-Cas nucleases designed to bias their inheritance and rapidly... Read More about A genetically encoded anti-CRISPR protein constrains gene drive spread and prevents population suppression.

Genetic Technologies for Sustainable Management of Insect Pests and Disease Vectors (2021)
Journal Article
Grilli, S., Galizi, R., & Taxiarchi, C. (2021). Genetic Technologies for Sustainable Management of Insect Pests and Disease Vectors. Sustainability, 5653 - 5653. https://doi.org/10.3390/su13105653

Recent advancements in genetic and genome editing research, augmented by the discovery of new molecular tools such as CRISPR, have revolutionised the field of genetic engineering by enabling precise site-specific genome modifications with unprecedent... Read More about Genetic Technologies for Sustainable Management of Insect Pests and Disease Vectors.

A Code of Ethics for Gene Drive Research. (2021)
Journal Article
Annas, G. J., Beisel, C. L., Clement, K., Crisanti, A., Francis, S., Galardini, M., …Keith Joung, J. (2021). A Code of Ethics for Gene Drive Research. The CRISPR Journal, 19-24. https://doi.org/10.1089/crispr.2020.0096

Gene drives hold promise for use in controlling insect vectors of diseases, agricultural pests, and for conservation of ecosystems against invasive species. At the same time, this technology comes with potential risks that include unknown downstream... Read More about A Code of Ethics for Gene Drive Research..

Engineering Adaptive Gene Circuits in Bacteria Mastering Game Playing by Reinforcement Learning (2021)
Journal Article
Prakash, S., Racovita, A., Varela, C., Walsh, M., Galizi, R., Isalan, M., & Jaramillo, A. (2021). Engineering Adaptive Gene Circuits in Bacteria Mastering Game Playing by Reinforcement Learning. Biophysical Journal, 262A - 262A. https://doi.org/10.1016/j.bpj.2020.11.1683

Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance (2021)
Journal Article
Hammond, A., Karlsson, X., Morianou, I., Kyrou, K., Beaghton, A., Gribble, M., …Nolan, T. (2021). Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance. PLoS Genetics, e1009321 - ?. https://doi.org/10.1371/journal.pgen.1009321

Homing-based gene drives use a germline source of nuclease to copy themselves at specific target sites in a genome and bias their inheritance. Such gene drives can be designed to spread and deliberately suppress populations of malaria mosquitoes by i... Read More about Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance.

A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae (2020)
Journal Article
Simoni, A., Hammond, A. M., Beaghton, A. K., Galizi, R., Taxiarchi, C., Kyrou, K., …Crisanti, A. (2020). A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae. Nature Biotechnology, https://doi.org/10.1038/s41587-020-0508-1

Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce uni... Read More about A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae.

Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics (2020)
Journal Article
Galizi, R., Duncan, J. N., Rostain, W., Quinn, C. M., Storch, M., Kushwaha, M., & Jaramillo, A. (2020). Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics. The CRISPR Journal, 398 - 408. https://doi.org/10.1089/crispr.2020.0029

CRISPR guide RNAs (gRNAs) can be programmed with relative ease to allow the genetic editing of nearly any DNA or RNA sequence. Here, we propose novel molecular architectures to achieve RNA-dependent modulation of CRISPR activity in response to specif... Read More about Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics.

Vector-Focused Approaches to Curb Malaria Transmission in the Brazilian Amazon: An Overview of Current and Future Challenges and Strategies (2020)
Journal Article
Matos Rocha, E., de Melo Katak, R., Campos de Oliveira, J., da Silva Araujo, M., Cechetto Carlos, B., Galizi, R., …A. Souza-Neto, J. (2020). Vector-Focused Approaches to Curb Malaria Transmission in the Brazilian Amazon: An Overview of Current and Future Challenges and Strategies. Tropical Medicine and Infectious Disease, 161. https://doi.org/10.3390/tropicalmed5040161

In Brazil, malaria transmission is mostly confined to the Amazon, where substantial progress has been achieved towards disease control in the past decade. Vector control has been historically considered a fundamental part of the main malaria control... Read More about Vector-Focused Approaches to Curb Malaria Transmission in the Brazilian Amazon: An Overview of Current and Future Challenges and Strategies.

Cellular mechanisms regulating synthetic sex ratio distortion in the Anopheles gambiae germline (2020)
Journal Article
Elaine Haghighat-Khah, R., Sharma, A., Reis Wunderlich, M., Morselli, G., Anna Marston, L., Bamikole, C., …Galizi, R. (2020). Cellular mechanisms regulating synthetic sex ratio distortion in the Anopheles gambiae germline. Pathogens and Global Health, 370 -378. https://doi.org/10.1080/20477724.2020.1803628

Genetic control strategies aimed to bias the sex of progenies towards males present a promising new paradigm to eliminate malaria-transmitting mosquitoes. A synthetic sex-ratio distortion (SD) system was successfully engineered in Anopheles gambiae b... Read More about Cellular mechanisms regulating synthetic sex ratio distortion in the Anopheles gambiae germline.