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All Outputs (30)

Interkingdom interactions shape the fungal microbiome of mosquitoes (2024)
Journal Article
Hegde, S., Khanipov, K., Hornett, E. A., Nilyanimit, P., Pimenova, M., Saldaña, M. A., de Bekker, C., Golovko, G., & Hughes, G. L. (2024). Interkingdom interactions shape the fungal microbiome of mosquitoes. Animal Microbiome, 6(1), Article 11. https://doi.org/10.1186/s42523-024-00298-4

Background
The mosquito microbiome is an important modulator of vector competence and vectoral capacity. Unlike the extensively studied bacterial microbiome, fungal communities in the mosquito microbiome (the mycobiome) remain largely unexplored. To... Read More about Interkingdom interactions shape the fungal microbiome of mosquitoes.

Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field‐caught or laboratory‐reared mosquitoes (2024)
Journal Article
Hegde, S., Brettell, L. E., Quek, S., Etebari, K., Saldaña, M. A., Asgari, S., Coon, K. L., Heinz, E., & Hughes, G. L. (2024). Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field‐caught or laboratory‐reared mosquitoes. Environmental Microbiology, 26(2), Article e16576. https://doi.org/10.1111/1462-2920.16576

The mosquito microbiome is critical for host development and plays a major role in many aspects of mosquito biology. While the microbiome is commonly dominated by a small number of genera, there is considerable variation in composition among mosquito... Read More about Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field‐caught or laboratory‐reared mosquitoes.

Identification and characterization of two CRISPR-Cas systems associated with the mosquito microbiome (2023)
Journal Article
Hegde, S., Rauch, H. E., Hughes, G. L., & Shariat, N. (in press). Identification and characterization of two CRISPR-Cas systems associated with the mosquito microbiome. Access Microbiology, 5(8), https://doi.org/10.1099/acmi.0.000599.v3

The microbiome profoundly influences many traits in medically relevant vectors such as mosquitoes, and a greater functional understanding of host-microbe interactions may be exploited for novel microbial-based approaches to control mosquito-borne dis... Read More about Identification and characterization of two CRISPR-Cas systems associated with the mosquito microbiome.

Identification and characterization of two CRISPR/Cas systems associated with the mosquito microbiome (2023)
Journal Article
Hegde, S., Rauch, H. E., Hughes, G. L., & Shariat, N. (2023). Identification and characterization of two CRISPR/Cas systems associated with the mosquito microbiome. Access Microbiology, 5(8), https://doi.org/10.1099/acmi.0.000599.v4

The microbiome profoundly influences many traits in medically relevant vectors such as mosquitoes, and a greater functional understanding of host–microbe interactions may be exploited for novel microbial-based approaches to control mosquito-borne dis... Read More about Identification and characterization of two CRISPR/Cas systems associated with the mosquito microbiome.

The 2022 dengue outbreak in Bangladesh: hypotheses for the late resurgence of cases and fatalities (2023)
Journal Article
Haider, N., N, H., Hasan, M. N., MN, H., Khalil, I., I, K., Tonge, D., D, T., Hegde, S., S, H., Chowdhury, M. A. B., MAB, C., Rahman, M., M, R., Khan, M. H., MH, K., Ansumana, R., R, A., Zumla, A., A, Z., …MJ, U. (2023). The 2022 dengue outbreak in Bangladesh: hypotheses for the late resurgence of cases and fatalities. Journal of Medical Entomology, 60(4), 847-852. https://doi.org/10.1093/jme/tjad057

Bangladesh reported the highest number of annual deaths (n = 281) related to dengue virus infection in 2022 since the virus reappeared in the country in 2000. Earlier studies showed that >92% of the annual cases occurred between the months of... Read More about The 2022 dengue outbreak in Bangladesh: hypotheses for the late resurgence of cases and fatalities.

Aedes aegyptigut transcriptomes respond differently to microbiome transplants from field-caught or laboratory-reared mosquitoes (2023)
Preprint / Working Paper
Hegde, S., Brettell, L. E., Quek, S., Etebari, K., Saldaña, M. A., Asgari, S., Coon, K. L., Heinz, E., & Hughes, G. L. Aedes aegyptigut transcriptomes respond differently to microbiome transplants from field-caught or laboratory-reared mosquitoes

The mosquito microbiome is critical for host development and plays a major role in many aspects of mosquito biology. While the microbiome is commonly dominated by a small number of genera, there is considerable variation in composition among mosquito... Read More about Aedes aegyptigut transcriptomes respond differently to microbiome transplants from field-caught or laboratory-reared mosquitoes.

Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes (2022)
Journal Article
Coon, K., Hegde, S., & Hughes, G. (2022). Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes. Microbiome, 10, Article 58. https://doi.org/10.1186/s40168-022-01256-5

Background Mosquitoes harbor microbial communities that play important roles in their growth, survival, reproduction, and ability to transmit human pathogens. Microbiome transplantation approaches are often used to study host-microbe interactions and... Read More about Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes.

Vertical and Horizontal Transmission of Cell Fusing Agent Virus in Aedes aegypti (2022)
Journal Article
Logan, R., Quek, S., Muthoni, J., von Eicken, A., Brettell, L., Anderson, E., Villena, M., Hegde, S., Patterson, G., Heinz, E., Hughes, G., & Patterson, E. (2022). Vertical and Horizontal Transmission of Cell Fusing Agent Virus in Aedes aegypti. Applied and Environmental Microbiology, https://doi.org/10.1128/aem.01062-22

Cell fusing agent virus (CFAV) is an insect-specific flavivirus (ISF) found in Aedes aegypti mosquitoes. ISFs have demonstrated the ability to modulate the infection or transmission of arboviruses such as dengue, West Nile, and Zika viruses. It is th... Read More about Vertical and Horizontal Transmission of Cell Fusing Agent Virus in Aedes aegypti.

Transcriptomic and small RNA response to Mayaro virus infection in Anopheles stephensi mosquitoes (2022)
Journal Article
Henderson, C., Brustolin, M., Dayama, G., Lau, N., Hughes, G., Bergey, C., L. Hughes, G., Rasgon, J., Hegde, S., & L. Rasgon, J. (2022). Transcriptomic and small RNA response to Mayaro virus infection in Anopheles stephensi mosquitoes. PLoS Neglected Tropical Diseases, 1 - 21. https://doi.org/10.1371/journal.pntd.0010507

<jats:p>Mayaro virus (MAYV) is an arboviral pathogen in the genus <jats:italic>Alphavirus</jats:italic> that is circulating in South America with potential to spread to naïve regions. MAYV is also one of the few viruses with the ability to be transmi... Read More about Transcriptomic and small RNA response to Mayaro virus infection in Anopheles stephensi mosquitoes.

Inter-species microbiota transplantation recapitulates microbial acquisition and persistence in mosquitoes (2021)
Preprint / Working Paper
Coon, K. L., Hegde, S., & Hughes, G. L. Inter-species microbiota transplantation recapitulates microbial acquisition and persistence in mosquitoes

Background Mosquitoes harbor microbial communities that play important roles in their growth, survival, reproduction, and ability to transmit human pathogens. Microbiome transplantation approaches are often used to study host-microbe interactions and... Read More about Inter-species microbiota transplantation recapitulates microbial acquisition and persistence in mosquitoes.

Stable high-density and maternally inherited Wolbachia infections in Anopheles moucheti and Anopheles demeilloni mosquitoes (2021)
Journal Article
Walker, T., Quek, S., Jeffries, C. L., Bandibabone, J., Dhokiya, V., Bamou, R., Kristan, M., Messenger, L. A., Gidley, A., Hornett, E. A., Anderson, E. R., Cansado-Utrilla, C., Hegde, S., Bantuzeko, C., Stevenson, J. C., Lobo, N. F., Wagstaff, S. C., Nkondjio, C. A., Irish, S. R., Heinz, E., & Hughes, G. L. (2021). Stable high-density and maternally inherited Wolbachia infections in Anopheles moucheti and Anopheles demeilloni mosquitoes. Current Biology, 31(11), 2310-2320.e5. https://doi.org/10.1016/j.cub.2021.03.056

Microbial interactions in the mosquito gut determine Serratia colonization and blood feeding propensity. (2020)
Preprint / Working Paper
Kozlova, E. V., Hegde, S., Roundy, C. M., Golovko, G., A Saldana, M., Hart, C. E., Anderson, E. R., Hornett, E. A., Khanipov, K., Popov, V. L., Pimenova, M., Zhou, Y., Fovanov, Y., Weaver, S. C., Routh, A. L., Heinz, E., & Hughes, G. L. Microbial interactions in the mosquito gut determine Serratia colonization and blood feeding propensity

How microbe-microbe interactions dictate microbial complexity in the mosquito gut is unclear. Previously we found that Serratia, a gut symbiont that alters vector competence and is being considered for vector control, poorly colonized Aedes aegypti y... Read More about Microbial interactions in the mosquito gut determine Serratia colonization and blood feeding propensity..

Gut-associated bacteria invade the midgut epithelium of Aedes aegypti and stimulate innate immunity and suppress Zika virus infection in cells (2020)
Preprint / Working Paper
Hegde, S., Voronin, D., Casas-Sanchez, A., Saldaña, M. A., Heinz, E., Acosta-Serrano, A., Popov, V. L., Chopra, A. K., & Hughes, G. L. Gut-associated bacteria invade the midgut epithelium of Aedes aegypti and stimulate innate immunity and suppress Zika virus infection in cells

Microbiota within mosquitoes influence nutrition, immunity, fecundity, and the capacity to transmit pathogens. Despite their importance, we have a limited understanding of host-microbiota interactions, especially at the cellular level. It is evident... Read More about Gut-associated bacteria invade the midgut epithelium of Aedes aegypti and stimulate innate immunity and suppress Zika virus infection in cells.

CRISPR/Cas9-mediated gene deletion of the ompA gene in symbiotic Cedecea neteri impairs biofilm formation and reduces gut colonization of Aedes aegypti mosquitoes (2019)
Journal Article
Hegde, S., Nilyanimit, P., Kozlova, E., Anderson, E. R., Narra, H. P., Sahni, S. K., Heinz, E., & Hughes, G. L. (2019). CRISPR/Cas9-mediated gene deletion of the ompA gene in symbiotic Cedecea neteri impairs biofilm formation and reduces gut colonization of Aedes aegypti mosquitoes. PLoS Neglected Tropical Diseases, 13(12), e0007883. https://doi.org/10.1371/journal.pntd.0007883

Novel wolbachia strains in anopheles malaria vectors from sub-saharan africa [version 2; referees: 3 approved] (2018)
Journal Article
Jeffries, C. L., Lawrence, G. G., Golovko, G., Kristan, M., Orsborne, J., Spence, K., Hurn, E., Bandibabone, J., Tantely, L. M., Raharimalala, F. N., Keita, K., Camara, D., Barry, Y., Wat’senga, F., Manzambi, E. Z., Afrane, Y. A., Mohammed, A. R., Abeku, T. A., Hegde, S., Khanipov, K., …Walker, T. (2018). Novel wolbachia strains in anopheles malaria vectors from sub-saharan africa [version 2; referees: 3 approved]. Wellcome Open Research, 3(113), https://doi.org/10.12688/wellcomeopenres.14765.2

Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gamb... Read More about Novel wolbachia strains in anopheles malaria vectors from sub-saharan africa [version 2; referees: 3 approved].

Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa (2018)
Journal Article
Jeffries, C. L., Lawrence, G. G., Golovko, G., Kristan, M., Orsborne, J., Spence, K., Hurn, E., Bandibabone, J., Tantely, L. M., Raharimalala, F. N., Keita, K., Camara, D., Barry, Y., Wat’senga, F., Manzambi, E. Z., Afrane, Y. A., Mohammed, A. R., Abeku, T. A., Hegde, S., Khanipov, K., …Walker, T. (2018). Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa. Wellcome Open Research, 3(113),

Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gamb... Read More about Novel Wolbachia strains in Anopheles malaria vectors from Sub-Saharan Africa.

Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-Collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus Mosquito Vectors (2018)
Journal Article
Hegde, S., Khanipov, K., Albayrak, L., Golovko, G., Pimenova, M., Saldaña, M. A., Rojas, M. M., Hornett, E. A., Motl, G. C., Fredregill, C. L., Dennett, J. A., Debboun, M., Fofanov, Y., & Hughes, G. L. (in press). Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-Collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus Mosquito Vectors. Frontiers in Microbiology, 9, https://doi.org/10.3389/fmicb.2018.02160

Microbial interactions are an underappreciated force in shaping insect microbiome communities. Although pairwise patterns of symbiont interactions have been identified, we have a poor understanding regarding the scale and the nature of co-occurrence... Read More about Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-Collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus Mosquito Vectors.