Transcriptomic and small RNA response to Mayaro virus infection in Anopheles stephensi mosquitoes

Henderson, Cory; Brustolin, Marco; Dayama, Gargi; Lau, Nelson; Hughes, GL; Bergey, Christina; Rasgon, JL; Hegde, Shivanand

Abstract

<jats:p>Mayaro virus (MAYV) is an arboviral pathogen in the genus <jats:italic>Alphavirus</jats:italic> that is circulating in South America with potential to spread to naïve regions. MAYV is also one of the few viruses with the ability to be transmitted by mosquitoes in the genus <jats:italic>Anopheles</jats:italic>, as well as the typical arboviral transmitting mosquitoes in the genus <jats:italic>Aedes</jats:italic>. Few studies have investigated the infection response of <jats:italic>Anopheles</jats:italic> mosquitoes. In this study we detail the transcriptomic and small RNA responses of <jats:italic>An</jats:italic>. <jats:italic>stephensi</jats:italic> to infection with MAYV via infectious bloodmeal at 2, 7, and 14 days post infection (dpi). 487 unique transcripts were significantly regulated, 78 putative novel miRNAs were identified, and an siRNA response is observed targeting the MAYV genome. Gene ontology analysis of transcripts regulated at each timepoint shows a number of proteases regulated at 2 and 7 dpi, potentially representative of Toll or melanization pathway activation, and repression of pathways related to autophagy and apoptosis at 14 dpi. These findings provide a basic understanding of the infection response of <jats:italic>An</jats:italic>. <jats:italic>stephensi</jats:italic> to MAYV and help to identify host factors which might be useful to target to inhibit viral replication in <jats:italic>Anopheles</jats:italic> mosquitoes.</jats:p>

Citation

Henderson, C., Brustolin, M., Dayama, G., Lau, N., Hughes, G., Bergey, C., …L. Rasgon, J. (2022). Transcriptomic and small RNA response to Mayaro virus infection in Anopheles stephensi mosquitoes. PLoS Neglected Tropical Diseases, 1 - 21. https://doi.org/10.1371/journal.pntd.0010507

• Journal Article Type: Article
• Acceptance Date: May 17, 2022
• Publication Date: Jun 28, 2022
• Journal: PLOS Neglected Tropical Diseases
• Publisher: Public Library of Science (PLoS)
• Pages: 1 - 21
• DOI: https://doi.org/10.1371/journal.pntd.0010507
• Public URL: https://keele-repository.worktribe.com/output/424805
• Publisher URL: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0010507