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All Outputs (34)

Genetically engineered distal airway stem cell transplantation protects mice from pulmonary infection (2020)
Journal Article
Dale, T., & Forsyth, N. (2020). Genetically engineered distal airway stem cell transplantation protects mice from pulmonary infection. EMBO Molecular Medicine, https://doi.org/10.15252/emmm.201810233

Severe pulmonary infection is a major threat to human health accompanied by substantial, which increases medical costs, prolonged inpatient requirements, and high mortality rates. New anti-microbial therapeutic strategies are urgently required to add... Read More about Genetically engineered distal airway stem cell transplantation protects mice from pulmonary infection.

Rock Inhibitor Y-27632 Enables Feeder-Free, Unlimited Expansion of Sus scrofa domesticus Swine Airway Stem Cells to Facilitate Respiratory Research (2019)
Journal Article
Dale, T. P., Borg D’anastasi, E., Haris, M., & Forsyth, N. R. (2019). Rock Inhibitor Y-27632 Enables Feeder-Free, Unlimited Expansion of Sus scrofa domesticus Swine Airway Stem Cells to Facilitate Respiratory Research. Stem Cells International, 2019, 1 - 15. https://doi.org/10.1155/2019/3010656

Current limitations in the efficacy of treatments for chronic respiratory disorders position them as prospective regenerative medicine therapeutic targets. A substantial barrier to these ambitions is that research requires large numbers of cells whos... Read More about Rock Inhibitor Y-27632 Enables Feeder-Free, Unlimited Expansion of Sus scrofa domesticus Swine Airway Stem Cells to Facilitate Respiratory Research.

Hypoxia-modified cancer cell metabolism (2019)
Journal Article
Al Tameemi, W., Dale, T. P., Kh Al-Jumaily, R. M., & Forsyth, N. R. (2019). Hypoxia-modified cancer cell metabolism. Frontiers in Cell and Developmental Biology, 7(4), https://doi.org/10.3389/fcell.2019.00004

While oxygen is critical to the continued existence of complex organisms, extreme levels of oxygen within a system, known as hypoxia (low levels of oxygen) and hyperoxia (excessive levels of oxygen), potentially promote stress within a defined biolog... Read More about Hypoxia-modified cancer cell metabolism.

Ectopic Telomerase Expression Fails to Maintain Chondrogenic Capacity in Three-Dimensional Cultures of Clinically Relevant Cell Types. (2018)
Journal Article
Dale, T. P., & Forsyth, N. R. (in press). Ectopic Telomerase Expression Fails to Maintain Chondrogenic Capacity in Three-Dimensional Cultures of Clinically Relevant Cell Types. BioResearch Open Access, 7(1), 10 - 24. https://doi.org/10.1089/biores.2018.0008

The poor healing capacity of cartilage and lack of effective treatment for associated disease and trauma makes it a strong candidate for a regenerative medicine approach. Potential therapies tested to date, although effective, have met with a number... Read More about Ectopic Telomerase Expression Fails to Maintain Chondrogenic Capacity in Three-Dimensional Cultures of Clinically Relevant Cell Types..

Tenogenic differentiation of human embryonic stem cells (2018)
Journal Article
Dale, T. P., Mazher, S., Webb, W. R., Zhou, J., Maffulli, N., Chen, G.-Q., El Haj, A. J., & Forsyth, N. R. (2018). Tenogenic differentiation of human embryonic stem cells. Tissue engineering. Part A, 24(5-6), 361-368. https://doi.org/10.1089/ten.TEA.2017.0017

Tendon healing is complex to manage because of the limited regeneration capacity of tendon tissue; stem cell-based tissue engineering approaches may provide alternative healing strategies. We sought to determine whether human embryonic stem cells (hE... Read More about Tenogenic differentiation of human embryonic stem cells.

Pluripotent and Multipotent Stem Cells Display Distinct Hypoxic miRNA Expression Profiles (2016)
Journal Article
Agrawal, R., Dale, T. P., Al-Zubaidi, M. A., Benny Malgulwar, P., Forsyth, N. R., & Kulshreshtha, R. (in press). Pluripotent and Multipotent Stem Cells Display Distinct Hypoxic miRNA Expression Profiles. PloS one, 11(10), e0164976. https://doi.org/10.1371/journal.pone.0164976

MicroRNAs are reported to have a crucial role in the regulation of self-renewal and differentiation of stem cells. Hypoxia has been identified as a key biophysical element of the stem cell culture milieu however, the link between hypoxia and miRNA ex... Read More about Pluripotent and Multipotent Stem Cells Display Distinct Hypoxic miRNA Expression Profiles.

Self-renewal of human embryonic stem cells on defined synthetic electrospun nanofibers (2015)
Journal Article
Kumar, D., Dale, T. P., Yang, Y., & Forsyth, N. R. (2015). Self-renewal of human embryonic stem cells on defined synthetic electrospun nanofibers. Biomedical Materials, 10(6), Article 065017. https://doi.org/10.1088/1748-6041/10/6/065017

Human embryonic stem cells (hESCs) are conventionally expanded and maintained in vitro on biological substrates. Synthetic electrospun polymer nanofibers have the potential to act as non-biological substrates in the culture of hESCs. Three synthetic,... Read More about Self-renewal of human embryonic stem cells on defined synthetic electrospun nanofibers.

Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner (2015)
Journal Article
Dale, T. P., de Castro, A., Kuiper, N. J., Parkinson, E. K., & Forsyth, N. R. (2015). Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner. PloS one, 10(7), Article e0133745. https://doi.org/10.1371/journal.pone.0133745

BACKGROUND: Limited options for the treatment of cartilage damage have driven the development of tissue engineered or cell therapy alternatives reliant on ex vivo cell expansion. The study of chondrogenesis in primary cells is difficult due to progre... Read More about Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner.

Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner. (2015)
Journal Article
Dale, T. P., de Castro, A., Kuiper, N. J., Parkinson, E. K., & Forsyth, N. R. (2015). Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner. PloS one, 10(7), Article e0133745. https://doi.org/10.1371/journal.pone.0133745

BACKGROUND: Limited options for the treatment of cartilage damage have driven the development of tissue engineered or cell therapy alternatives reliant on ex vivo cell expansion. The study of chondrogenesis in primary cells is difficult due to progre... Read More about Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner..

BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation. (2015)
Journal Article
Gawain Kay, A., Dale, T., Mehedi Akram, K., Mohan, P., Hampson, K., Maffulli, N., Spiteri, M. A., El Haj, A., & Forsyth, N. (2015). BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation. Regenerative Medicine, 10(2), 109 - 125. https://doi.org/10.2217/rme.14.67

AIM: Human mesenchymal stem cells (hMSC) are multipotent progenitor cells. We propose the optimization of hMSC isolation and recovery using the application of a controlled hypoxic environment. MATERIALS & METHODS: We evaluated oxygen, glucose and ser... Read More about BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation..

Immortalisation with hTERT impacts on sulphated glycosaminoglycan secretion and chondrogenic potential in a variable and cell specific manner (2014)
Presentation / Conference Contribution
Dale, T. P., De Castro, A., Parkinson, E. K., Kuiper, N. J., & Forsyth, N. R. (2014, June). Immortalisation with hTERT impacts on sulphated glycosaminoglycan secretion and chondrogenic potential in a variable and cell specific manner. Poster presented at Tissue Engineering & Regenerative Medicine International Society, European Chapter Meeting, Magazzini del Cotone Congress Center, Genova, Italy

Introduction: Limited therapeutic options for the treatment of cartilage damage have driven the development of cell therapy alternatives. How-ever, many of the relevant cell types are difficult to study due to the rapid onset of replicative senescenc... Read More about Immortalisation with hTERT impacts on sulphated glycosaminoglycan secretion and chondrogenic potential in a variable and cell specific manner.

The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model (2013)
Journal Article
Webb, W. R., Dale, T. P., Lomas, A. J., Zeng, G., Wimpenny, I., El Haj, A. J., Forsyth, N. R., & Chen, G.-Q. (2013). The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model. Biomaterials, 34(28), 6683-6694. https://doi.org/10.1016/j.biomaterials.2013.05.041

Tendon injuries and defects present a substantial burden to global healthcare economies. There are no synthetic/biosynthesised implants available which can restore full function or match the mechanical properties of native tendon. Therefore, poly(3-h... Read More about The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model.

Investigating the chondrogenic phenotype in clinically relevant cells: the effect of hTERT expression
Thesis
Dale, T. P. Investigating the chondrogenic phenotype in clinically relevant cells: the effect of hTERT expression. https://keele-repository.worktribe.com/output/407215

Damaged or diseased mature articular cartilage cannot undergo effective tissue repair and due to its avascular, hypocellular nature defects become widespread and painful. No ‘gold standard’ treatment exists for this indication and the ultimate recour... Read More about Investigating the chondrogenic phenotype in clinically relevant cells: the effect of hTERT expression.