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BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation.

Gawain Kay, Alasdair; Dale, Tina; Mehedi Akram, Khondoker; Mohan, Param; Hampson, Karen; Maffulli, Nicola; Spiteri, Monica A; El Haj, Alicia; Forsyth, Nicholas


Alasdair Gawain Kay

Khondoker Mehedi Akram

Param Mohan

Karen Hampson

Nicola Maffulli

Monica A Spiteri

Alicia El Haj

Nicholas Forsyth


AIM: Human mesenchymal stem cells (hMSC) are multipotent progenitor cells. We propose the optimization of hMSC isolation and recovery using the application of a controlled hypoxic environment. MATERIALS & METHODS: We evaluated oxygen, glucose and serum in the recovery of hMSC from bone marrow (BMhMSC). Colony forming units-fibroblastic, cell numbers, tri-lineage differentiation, immunofluorescence and microarray were used to confirm and characterize BMhMSC. RESULTS: In an optimized (2% O(2), 4.5 g/l glucose and 5% serum) environment both colony forming units-fibroblastic (p = 0.01) and cell numbers (p = 0.0001) were enhanced over standard conditions. Transcriptional analysis identified differential expression of bone morphogenetic protein 2 (BMP2) and, putatively, chemokine (C-X-C motif) receptor 2 (CXCR2) signaling pathways. CONCLUSION: We have detailed a potential milestone in the process of refinement of the BMhMSC isolation process.

Journal Article Type Article
Online Publication Date Apr 2, 2015
Publication Date 2015-03
Publicly Available Date May 26, 2023
Journal Regen Med
Print ISSN 1746-0751
Publisher Future Medicine
Peer Reviewed Peer Reviewed
Volume 10
Issue 2
Pages 109 - 125
Keywords Mesenchymal stem cells, physiological normoxia, hypoxia, BMP2, transcriptome, CFU-F
Publisher URL