All Outputs (25)

Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner. (2015)
Journal Article
Dale, T. P., de Castro, A., Kuiper, N. J., Parkinson, E. K., & Forsyth, N. R. (2015). Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner. PloS one, 10(7), Article e0133745. https://doi.org/10.1371/journal.pone.0133745

BACKGROUND: Limited options for the treatment of cartilage damage have driven the development of tissue engineered or cell therapy alternatives reliant on ex vivo cell expansion. The study of chondrogenesis in primary cells is difficult due to progre... Read More about Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner..

BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation. (2015)
Journal Article
Gawain Kay, A., Dale, T., Mehedi Akram, K., Mohan, P., Hampson, K., Maffulli, N., …Forsyth, N. (2015). BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation. Regenerative Medicine, 10(2), 109 - 125. https://doi.org/10.2217/rme.14.67

AIM: Human mesenchymal stem cells (hMSC) are multipotent progenitor cells. We propose the optimization of hMSC isolation and recovery using the application of a controlled hypoxic environment. MATERIALS & METHODS: We evaluated oxygen, glucose and ser... Read More about BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation..

Immortalisation with hTERT impacts on sulphated glycosaminoglycan secretion and chondrogenic potential in a variable and cell specific manner (2014)
Presentation / Conference
Dale, T. P., De Castro, A., Parkinson, E. K., Kuiper, N. J., & Forsyth, N. R. (2014, June). Immortalisation with hTERT impacts on sulphated glycosaminoglycan secretion and chondrogenic potential in a variable and cell specific manner. Poster presented at Tissue Engineering & Regenerative Medicine International Society, European Chapter Meeting, Magazzini del Cotone Congress Center, Genova, Italy

Introduction: Limited therapeutic options for the treatment of cartilage damage have driven the development of cell therapy alternatives. How-ever, many of the relevant cell types are difficult to study due to the rapid onset of replicative senescenc... Read More about Immortalisation with hTERT impacts on sulphated glycosaminoglycan secretion and chondrogenic potential in a variable and cell specific manner.

The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model (2013)
Journal Article
Webb, W. R., Dale, T. P., Lomas, A. J., Zeng, G., Wimpenny, I., El Haj, A. J., …Chen, G. (2013). The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model. Biomaterials, 34(28), 6683-6694. https://doi.org/10.1016/j.biomaterials.2013.05.041

Tendon injuries and defects present a substantial burden to global healthcare economies. There are no synthetic/biosynthesised implants available which can restore full function or match the mechanical properties of native tendon. Therefore, poly(3-h... Read More about The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model.

Investigating the chondrogenic phenotype in clinically relevant cells: the effect of hTERT expression
Thesis
Dale, T. P. (2016). Investigating the chondrogenic phenotype in clinically relevant cells: the effect of hTERT expression

Damaged or diseased mature articular cartilage cannot undergo effective tissue repair and due to its avascular, hypocellular nature defects become widespread and painful. No ‘gold standard’ treatment exists for this indication and the ultimate recour... Read More about Investigating the chondrogenic phenotype in clinically relevant cells: the effect of hTERT expression.