All Outputs (5)

Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes. (2018)
Journal Article
Bernardini, F., Elaine Haghighat-Khah, R., Galizi, R., Marc Hammond, A., Nolan, T., & Crisanti, A. (2018). Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes. Parasites and Vectors, 660 - ?. https://doi.org/10.1186/s13071-018-3207-8

Malaria is a serious global health burden, affecting more than 200 million people each year in over 90 countries, predominantly in Africa, Asia and the Americas. Since the year 2000, a concerted effort to combat malaria has reduced its incidence by m... Read More about Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes..

Engineering CRISPR guide RNA riboswitches for in vivo applications (2018)
Journal Article
Galizi, R., & Jaramillo, A. (2018). Engineering CRISPR guide RNA riboswitches for in vivo applications. Current Opinion in Biotechnology, 103 - 113. https://doi.org/10.1016/j.copbio.2018.08.007

CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and biotechnology applications. Whilst the fundamental properties of CRISPR proved easily transferable from the native prokaryotic hosts to eukaryotic and... Read More about Engineering CRISPR guide RNA riboswitches for in vivo applications.

A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes (2018)
Journal Article
Kyrou, K., M Hammond, A., Galizi, R., Kranjc, N., Burt, A., K Beaghton, A., …Crisanti, A. (2018). A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes. Nature Biotechnology, 1062 - 1066. https://doi.org/10.1038/nbt.4245

In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control differentiation of the two sexes. The female transcript, unlike the male,... Read More about A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes.

Improved CRISPR-based suppression gene drives mitigate resistance and impose a large reproductive load on laboratory-contained mosquito populations (2018)
Journal Article
Hammond, A. M., Kyrou, K., Gribble, M., Karlsson, X., Morianou, I., Galizi, R., …Nolan, T. (2018). Improved CRISPR-based suppression gene drives mitigate resistance and impose a large reproductive load on laboratory-contained mosquito populations. bioRxiv,

Abstract CRISPR-based genes drives bias their own inheritance and can be used to modify entire populations of insect vectors of disease as a novel form of sustainable disease control. Gene drives designed to interfere with female fertility can suppre... Read More about Improved CRISPR-based suppression gene drives mitigate resistance and impose a large reproductive load on laboratory-contained mosquito populations.

Gene drives to fight malaria: current state and future directions (2018)
Journal Article
Hammond, A. M., & Galizi, R. (2018). Gene drives to fight malaria: current state and future directions. Pathogens and Global Health, 412 - 423. https://doi.org/10.1080/20477724.2018.1438880

Self-propagating gene drive technologies have a number of desirable characteristics that warrant their development for the control of insect pest and vector populations, such as the malaria-transmitting mosquitoes. Theoretically easy to deploy and se... Read More about Gene drives to fight malaria: current state and future directions.