Outputs (18)

Label-Free In Situ Chemical Characterization of Amyloid Plaques in Human Brain Tissues (2024)
Journal Article
Everett, J., Brooks, J., Tjendana Tjhin, V., Lermyte, F., Hands-Portman, I., Plascencia-Villa, G., …Telling, N. D. (2024). Label-Free In Situ Chemical Characterization of Amyloid Plaques in Human Brain Tissues. ACS chemical neuroscience, 15(7), 1469–1483. https://doi.org/10.1021/acschemneuro.3c00756

The accumulation of amyloid plaques and increased brain redox burdens are neuropathological hallmarks of Alzheimer’s disease. Altered metabolism of essential biometals is another feature of Alzheimer’s, with amyloid plaques representing sites of dist... Read More about Label-Free In Situ Chemical Characterization of Amyloid Plaques in Human Brain Tissues.

Optical Microscopy Using the Faraday Effect Reveals in Situ Magnetization Dynamics of Magnetic Nanoparticles in Biological Samples (2024)
Journal Article
Sharifabad, M. E., Eizadi Sharifabad, M., Soucaille, R., Wang, X., Rotherham, M., Loughran, T., …Telling, N. (2024). Optical Microscopy Using the Faraday Effect Reveals in Situ Magnetization Dynamics of Magnetic Nanoparticles in Biological Samples. ACS Nano, 18(7), 5297-5310. https://doi.org/10.1021/acsnano.3c08955

The study of exogenous and endogenous nanoscale magnetic material in biology is important for developing biomedical nanotechnology as well as for understanding fundamental biological processes such as iron metabolism and biomineralization. Here, we e... Read More about Optical Microscopy Using the Faraday Effect Reveals in Situ Magnetization Dynamics of Magnetic Nanoparticles in Biological Samples.

Illuminating the brain: Revealing brain biochemistry with synchrotron X-ray spectromicroscopy (2023)
Journal Article
Everett, J., Brooks, J., Lermyte, F., Tjhin, V. T., Hands-Portman, I., Hill, E., …Telling, N. D. (2023). Illuminating the brain: Revealing brain biochemistry with synchrotron X-ray spectromicroscopy. Journal of Electron Spectroscopy and Related Phenomena, 266, Article ARTN 147355. https://doi.org/10.1016/j.elspec.2023.147355

The synchrotron x-ray spectromicroscopy technique Scanning Transmission X-ray Microscopy (STXM) offers a powerful means to examine the underlying biochemistry of biological systems, owing to its combined chemical sensitivity and nanoscale spatial res... Read More about Illuminating the brain: Revealing brain biochemistry with synchrotron X-ray spectromicroscopy.

Biogenic metallic elements in the human brain? (2021)
Journal Article
Everett, J., Lermyte, F., Brooks, J., Tjendana-Tjhin, V., Plascencia-Villa, G., Hands-Portman, I., …Telling, N. D. (2021). Biogenic metallic elements in the human brain?. Science Advances, 7(24), https://doi.org/10.1126/sciadv.abf6707

The chemistry of copper and iron plays a critical role in normal brain function. A variety of enzymes and proteins containing positively charged Cu+, Cu2+, Fe2+, and Fe3+ control key processes, catalyzing oxidative metabolism and neurotransmitter and... Read More about Biogenic metallic elements in the human brain?.

Nanoscale chemical speciation of ß-amyloid/iron aggregates using soft x-ray spectromicroscopy (2021)
Journal Article
Everett, J., Brooks, J., Collingwood, J. F., & Telling, N. (2021). Nanoscale chemical speciation of ß-amyloid/iron aggregates using soft x-ray spectromicroscopy. Inorganic Chemistry Frontiers, 8(6), 1439-1448. https://doi.org/10.1039/D0QI01304H

Iron (Fe) is an essential trace element required for healthy brain function. Yet, disrupted iron neurochemistry, and the associated formation of aberrantly aggregated protein lesions has been implicated in the development of multiple degenerative bra... Read More about Nanoscale chemical speciation of ß-amyloid/iron aggregates using soft x-ray spectromicroscopy.

Iron stored in ferritin is chemically reduced in the presence of aggregating Aß(1-42). (2020)
Journal Article
Everett, J., Brooks, J., Lermyte, F., O’Connor, P. B., Sadler, P. J., Dobson, J., …Telling, N. D. (2020). Iron stored in ferritin is chemically reduced in the presence of aggregating Aß(1-42). Scientific reports, 20, Article 10332. https://doi.org/10.1038/s41598-020-67117-z

Atypical low-oxidation-state iron phases in Alzheimer's disease (AD) pathology are implicated in disease pathogenesis, as they may promote elevated redox activity and convey toxicity. However, the origin of low-oxidation-state iron and the pathways r... Read More about Iron stored in ferritin is chemically reduced in the presence of aggregating Aß(1-42)..

Analysis of neuronal iron deposits in Parkinson's disease brain tissue by synchrotron x-ray spectromicroscopy. (2020)
Journal Article
Brooks, J., Everett, J., Lermyte, F., Tjendana Tjhin, V., Sadler, P. J., Telling, N., & Collingwood, J. F. (2020). Analysis of neuronal iron deposits in Parkinson's disease brain tissue by synchrotron x-ray spectromicroscopy. Journal of Trace Elements in Medicine and Biology, 62, Article 126555. https://doi.org/10.1016/j.jtemb.2020.126555

BACKGROUND: Neuromelanin-pigmented neurons, which are highly susceptible to neurodegeneration in the Parkinson's disease substantia nigra, harbour elevated iron levels in the diseased state. Whilst it is widely believed that neuronal iron is stored i... Read More about Analysis of neuronal iron deposits in Parkinson's disease brain tissue by synchrotron x-ray spectromicroscopy..

Label-Free Nanoimaging of Neuromelanin in the Brain by Soft X-ray Spectromicroscopy. (2020)
Journal Article
Telling, & Everett. (2020). Label-Free Nanoimaging of Neuromelanin in the Brain by Soft X-ray Spectromicroscopy. Angewandte Chemie International Edition, https://doi.org/10.1002/anie.202000239

A hallmark of Parkinson's disease is the death of neuromelanin-pigmented neurons, but the role of neuromelanin is unclear. The in situ characterization of neuromelanin remains dependent on detectable pigmentation, rather than direct quantification of... Read More about Label-Free Nanoimaging of Neuromelanin in the Brain by Soft X-ray Spectromicroscopy..

Emerging Approaches to Investigate the Influence of Transition Metals in the Proteinopathies (2019)
Journal Article
Telling, & Everett, J. (2019). Emerging Approaches to Investigate the Influence of Transition Metals in the Proteinopathies. Cells, https://doi.org/10.3390/cells8101231

Transition metals have essential roles in brain structure and function, and are associated with pathological processes in neurodegenerative disorders classed as proteinopathies. Synchrotron X-ray techniques, coupled with ultrahigh-resolution mass spe... Read More about Emerging Approaches to Investigate the Influence of Transition Metals in the Proteinopathies.

Metal Ion Binding to the Amyloid ß Monomer Studied by Native Top-Down FTICR Mass Spectrometry. (2019)
Journal Article
Lermyte, F., Everett, J., Lam, Y., Wootton, C., Brooks, J., Barrow, M., …Collingwood, J. (2019). Metal Ion Binding to the Amyloid ß Monomer Studied by Native Top-Down FTICR Mass Spectrometry. Journal of The American Society for Mass Spectrometry, 30(10), 2123-2134. https://doi.org/10.1007/s13361-019-02283-7

Native top-down mass spectrometry is a fast, robust biophysical technique that can provide molecular-scale information on the interaction between proteins or peptides and ligands, including metal cations. Here we have analyzed complexes of the full-l... Read More about Metal Ion Binding to the Amyloid ß Monomer Studied by Native Top-Down FTICR Mass Spectrometry..