Evaluation of bioluminescence-based assays of anti-malarial drug activity

Horrocks

Abstract

Transgenic Plasmodium falciparum expressing luciferase offers an attractive bioluminescence-based assay platform for the investigation of the pharmacological properties of anti-malarial drugs. Here a side-by-side comparison of bioluminescence and fluorescence-based assays, utilizing a luciferase reporter cassette that confers a strong temporal pattern of luciferase expression during the S-phase of intraerythrocytic development, is reported.

Citation

Horrocks. (2013). Evaluation of bioluminescence-based assays of anti-malarial drug activity. Malaria Journal, 58 - ?. https://doi.org/10.1186/1475-2875-12-58

• Acceptance Date: Feb 3, 2013
• Publication Date: Feb 8, 2013
• Journal: Malaria Journal
• Electronic ISSN: 1475-2875
• Publisher: Springer Verlag
• Pages: 58 - ?
• DOI: https://doi.org/10.1186/1475-2875-12-58
• Keywords: luciferase, sybr green I, antimalarial drug, relative rate of kill, malaria
• Public URL: https://keele-repository.worktribe.com/output/403448
• Publisher URL: http://www.malariajournal.com/content/12/1/58