An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Horrocks

Abstract

This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum. This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

Citation

Horrocks. (2012). An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum. Malaria Journal, 42 -?. https://doi.org/10.1186/1475-2875-11-42

• Acceptance Date: Feb 10, 2012
• Publication Date: Jan 1, 2012
• Journal: Malaria Journal
• Electronic ISSN: 1475-2875
• Publisher: Springer Verlag
• Pages: 42 -?
• DOI: https://doi.org/10.1186/1475-2875-11-42
• Keywords: luciferase, single-step lysis, stage-specific, promoter function, pcna
• Public URL: https://keele-repository.worktribe.com/output/403738
• Publisher URL: http://malariajournal.biomedcentral.com/articles/10.1186/1475-2875-11-42