Synthetic heparan sulfate dodecasaccharides reveal single sulfation site interconverts CXCL8 and CXCL12 chemokine biology

Jayson, Gordon C.; Hansen, Steen U.; Miller, Gavin J.; Cole, Claire L.; Rushton, Graham; Avizienytea, Egle; Gardiner, John M.

Abstract

he multigram-scale synthesis of a sulfation-site programmed heparin-like dodecasaccharide is described. Evaluation alongside dodecasaccharides lacking this single glucosamine O6-sulfation, or having per-O6-sulfation, shows that site-specific modification of the terminal glucosamine dramatically interconverts regulation of in vitro and in vivo biology mediated by the two important chemokines, CXCL12 (SDF1a) or CXCL8 (IL-8).

Citation

Jayson, G. C., Hansen, S. U., Miller, G. J., Cole, C. L., Rushton, G., Avizienytea, E., & Gardiner, J. M. (2015). Synthetic heparan sulfate dodecasaccharides reveal single sulfation site interconverts CXCL8 and CXCL12 chemokine biology. Chemical Communications, 51(72), 13846 -13849. https://doi.org/10.1039/c5cc05222j

• Journal Article Type: Article
• Acceptance Date: Jul 22, 2015
• Publication Date: Jul 22, 2015
• Journal: Chemical Communications
• Print ISSN: 1359-7345
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 51
• Issue: 72
• Pages: 13846 -13849
• DOI: https://doi.org/10.1039/c5cc05222j
• Publisher URL: http://dx.doi.org/10.1039/c5cc05222j