Divya Chari d.chari@keele.ac.uk
Development and characterisation of neurocompatible transfection grade magnetic nanoparticles for genetic modification of neural stem cells
Chari; Adams
Abstract
Genetic modification of cell transplant populations and cell tracking ability are key underpinnings for effective cell therapies. Current strategies to achieve these goals utilize methods which are unsuitable for clinical translation because of related safety issues, and multiple protocol steps adding to cost and complexity. Multifunctional magnetic nanoparticles (MNPs) offering dual mode gene delivery and imaging contrast capacity offer a valuable tool in this context. Despite their key benefits, there is a critical lack of neurocompatible and multifunctional particles described for use with transplant populations for neurological applications. Here, a systematic screen of MNPs (using a core shown to cause contrast in magnetic resonance imaging (MRI)) bearing various surface chemistries (polyethylenimine (PEI) and oxidized PEI and hybrids of oxidized PEI/alginic acid, PEI/chitosan and PEI/polyamidoamine) is performed to test their ability to genetically engineer neural stem cells (NSCs; a cell population of high clinical relevance for central nervous system disorders). It is demonstrated that gene delivery to NSCs can be safely achieved using two of the developed formulations (PEI and oxPEI/alginic acid) when used in conjunction with oscillating magnetofection technology. After transfection, intracellular particles can be detected by histological procedures with labeled cells displaying contrast in MRI (for real time cell tracking)
Citation
Chari, & Adams. (2016). Development and characterisation of neurocompatible transfection grade magnetic nanoparticles for genetic modification of neural stem cells. Advanced healthcare materials, 841-849. https://doi.org/10.1002/adhm.201500885
Acceptance Date | Jan 1, 2016 |
---|---|
Publication Date | Feb 11, 2016 |
Journal | Advanced Healthcare Materials |
Print ISSN | 2192-2640 |
Publisher | Wiley |
Pages | 841-849 |
DOI | https://doi.org/10.1002/adhm.201500885 |
Keywords | cell tracking, cell transplantation, magnetic nanoparticle, magnetic resonance imaging, magnetofection |
Publisher URL | http://dx.doi.org/10.1002/adhm.201500885 |
You might also like
Systematic Alignment Analysis of Neural Transplant Cells in Electrospun Nanofibre Scaffolds
(2022)
Journal Article
Electroactive Scaffolds to Improve Neural Stem Cell Therapy for Spinal Cord Injury
(2022)
Journal Article
Downloadable Citations
About Keele Repository
Administrator e-mail: research.openaccess@keele.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search