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Magnetic nanoparticle mediated transfection of neural stem cell suspension cultures is enhanced by applied oscillating magnetic fields.

Adams, CF; Pickard, MR; Chari, DM

Authors

MR Pickard



Abstract

UNLABELLED: Safe genetic modification of neural stem cell (NSC) transplant populations is a key goal for regenerative neurology. We describe a technically simple and safe method to increase transfection in NSCs propagated in the neurosphere (suspension culture) model, using magnetic nanoparticles deployed with applied oscillating magnetic fields ('magnetofection technology'). We show that transfection efficiency was enhanced over two-fold by oscillating magnetic fields (frequency=4 Hz). The protocols had no effect on cell viability, cell number, stem cell marker expression and differentiation profiles of 'magnetofected' cultures, highlighting the safety of the technique. As far as we are aware, this is the first successful application of magnetofection technology to suspension cultures of neural cells. The procedures described offer a means to augment the therapeutic potential of NSCs propagated as neurospheres - a culture model of high clinical translational relevance - by safe genetic manipulation, with further potential for incorporation into 'magneto-multifection' (repeat transfection) protocols. FROM THE CLINICAL EDITOR: This team of investigators describe a simple and safe method to increase transfection in neural stem cells using magnetic nanoparticles deployed with oscillating magnetic fields, demonstrating a greater than two-fold transfection efficiency increase by applying low frequency magnetic oscillation.

Citation

Adams, C., Pickard, M., & Chari, D. (2013). Magnetic nanoparticle mediated transfection of neural stem cell suspension cultures is enhanced by applied oscillating magnetic fields. Nanomedicine, 9, 737--741. https://doi.org/10.1016/j.nano.2013.05.014

Journal Article Type Article
Publication Date 2013
Deposit Date May 30, 2023
Journal Nanomedicine
Print ISSN 1743-5889
Electronic ISSN 1549-9642
Publisher Future Medicine
Peer Reviewed Peer Reviewed
Volume 9
Pages 737--741
DOI https://doi.org/10.1016/j.nano.2013.05.014
Keywords 4′,6-diamidino-2-phenylindole, CNS, DAPI, EGF, FGF-2, GFAP, GFP, MBP, MNP, Magnetic nanoparticle, NSC, Neural stem cell, Neurosphere, Nonviral, OPC, SEM, Transfection, Tuj-1, basic fibroblast growth factor, central nervous system, epidermal growth facto
Publisher URL https://www.ncbi.nlm.nih.gov/pubmed/23751375