William A. Woods
Developing a New Strategy for Delivery of Neural Transplant Populations Using Precursor Cell Sprays and Specialized Cell Media
Woods, William A.; Chowdhury, Farhana; Tzerakis, Nikolaos; Adams, Christopher F.; Chari, Divya M.
Authors
Farhana Chowdhury
Nikolaos Tzerakis
Christopher Adams c.adams@keele.ac.uk
Divya Chari d.chari@keele.ac.uk
Abstract
Neural precursor/stem cell transplantation therapies promote regeneration in neurological injuries, but current cell delivery methods have drawbacks. These include risks with surgical microinjection (e.g., hemorrhage, embolism) and high cell loss with systemic delivery/passage through fine gauge needles. Aerosolized cell delivery offers significant benefits including rapid and minimally invasive cell delivery, and ease of delivery to end users. To develop this approach, it is necessary to prove that 1) aerosolization does not have detrimental effects on transplant cells and 2) suitable media can be identified to support cell delivery. To achieve these aims, cells are sprayed using a commercial spray device or stored in Hibernate-A, a CO2-independent nutrient solution. Histological assessments consist of cell viability analysis, immunocytochemistry, and EdU labeling. It is shown that a major neural precursor transplant population-oligodendrocyte precursor cells (OPCs)-survive following aerosolized delivery and retain their capacity for proliferation and differentiation (key to their repair function). Hibernate-A can support OPCs' survival without specialized maintenance conditions, with no detrimental impact on cell fate. It is considered that this data supports the concept of a novel class of advanced medical spray devices to facilitate transport and delivery of transplant populations in neural cell therapy.
Citation
Woods, W. A., Chowdhury, F., Tzerakis, N., Adams, C. F., & Chari, D. M. (2021). Developing a New Strategy for Delivery of Neural Transplant Populations Using Precursor Cell Sprays and Specialized Cell Media. Advanced NanoBiomed Research, 1(9), 2100051 - 2100051. https://doi.org/10.1002/anbr.202100051
Journal Article Type | Article |
---|---|
Acceptance Date | May 25, 2021 |
Online Publication Date | Jul 11, 2021 |
Publication Date | Sep 1, 2021 |
Publicly Available Date | May 30, 2023 |
Journal | Advanced NanoBiomed Research |
Print ISSN | 2699-9307 |
Electronic ISSN | 2699-9307 |
Publisher | Wiley Open Access |
Volume | 1 |
Issue | 9 |
Pages | 2100051 - 2100051 |
DOI | https://doi.org/10.1002/anbr.202100051 |
Keywords | aerosolization, cell delivery, neural repair, stem cell spray, traumatic injury |
Publisher URL | https://doi.org/10.1002/anbr.202100051 |
Files
anbr.202100051.pdf
(561 Kb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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