Correlative Spectromicroscopy and Tomography for Biomedical Applications involving Electron, Ion, and Soft X-ray Microscopies

Telling, Neil; Everett, James

Abstract

Many important scientific and technical problems are best addressed using multiple, microscopy-based analytical techniques that combine the strengths of complementary methods. Here, we provide two examples from biomedical challenges: unravelling the attachment zone between dental implants and bone, and uncovering the mechanism of Alzheimer's disease. They combine synchrotron-based scanning transmission X-ray microscopy (STXM) with transmission electron microscopy ((S)TEM), electron tomography (ET), EELS tomography, and/or atom probe tomography (APT). STXM provides X-ray absorption based chemical sensitivity at mesoscale resolution (10–30 nm), which complements higher spatial resolution electron microscopy and APT.

• Acceptance Date: Mar 14, 2019
• Publication Date: Mar 14, 2019
• Journal: Microscopy Today
• Print ISSN: 1551-9295
• Publisher: Cambridge University Press
• DOI: https://doi.org/10.1017/S1551929518001256
• Keywords: scanning transmission X-ray microscopy; scanning transmission electron microscopy; atom probe tomography; titanium implants; Alzheimer's disease
• Publisher URL: https://www.cambridge.org/core/journals/microscopy-today/article/correlative-spectromicroscopy-and-tomography-for-biomedical-applications-involving-electron-ion-and-soft-xray-microscopies/1CFAC59353E5E2F0CBF685EE73AAB7C1