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Fourier transform infrared spectra of cells on glass coverslips: a further step in spectral pathology

Rutter, AV; Crees, J; Wright, H; van Pittius, DG; Yousef, I; Sule-Suso, J


J Crees

H Wright

DG van Pittius

I Yousef


Over the last few years, great effort has been placed on developing Fourier Transform Infrared (FTIR) microspectroscopy as a tool to help in the histopathological diagnosis of cancer. The ever increasing workload in pathology departments is calling for a technique that could identify the presence of cancer cells in cytology and tissue samples in an objective, fast and automated way. However, pathologists use glass slides which absorb infrared (IR) radiation thus removing important mid-IR spectral data in the fingerprint region (proteins, DNA, RNA; 1800 cm-1 to 900 cm-1). To this purpose, we hypothesised whether using thinner glass slides, i.e., glass coverslips, would allow us to obtain spectral data not only from the lipid region (3100 cm-1 to 2700 cm-1) but also from the fingerprint region. To this purpose, we studied peripheral blood mononuclear cells (PBMC), a leukaemia cell line (K562) and a lung cancer cell line (CALU-1). Cells were placed on DAKO coverslips and their FTIR spectra obtained at MIRAS beamline, Alba synchrotron light source (Barcelona, Catalonia). The data presented here not only shows for the first time that it is possible to obtain spectral data from most of the amide I region (1800 cm-1 to 1570 cm-1) of cells placed on glass coverslips but more important, principal component analysis was able to separate between the three types of cells for both the lipid and the amide I regions. The methodology here described is a further step in the application of FTIR microspectroscopy in histopathology departments.


Rutter, A., Crees, J., Wright, H., van Pittius, D., Yousef, I., & Sule-Suso, J. (2018). Fourier transform infrared spectra of cells on glass coverslips: a further step in spectral pathology. Analyst, 5711 - 5717.

Journal Article Type Article
Acceptance Date Oct 15, 2018
Online Publication Date Oct 17, 2018
Publication Date Nov 19, 2018
Publicly Available Date May 26, 2023
Journal Analyst
Print ISSN 0003-2654
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Pages 5711 - 5717
Keywords Cell Line, Tumor, Glass, Humans, Neoplasms, Principal Component Analysis, Spectroscopy, Fourier Transform Infrared
Publisher URL