Skip to main content

Research Repository

Advanced Search

Heparin Inhibits Cellular Invasion by SARS-CoV-2: Structural Dependence of the Interaction of the Spike S1 Receptor-Binding Domain with Heparin.

Mycroft-West, Courtney J.; Su, Dunhao; Pagani  , Isabel; Rudd  , Timothy R.; Elli  , Stefano; Gandhi  , Neha S.; Guimond  , Scott E.; Miller, Gavin J.; Meneghetti, Maria C.Z.; Nader, Helena B.; Li, Yong; Nunes, Quentin M.; Procter, Patricia; Mancini, Nicasio; Clementi, Massimo; Bisio, Antonella; Forsyth, Nicholas R.; Ferro, Vito; Turnbull  , Jeremy E.; Guerrini  , Marco; Fernig  , David G.; Vicenzi  , Elisa; Yates  , Edwin A.; Lima  , Marcelo A.; Skidmore   , Mark A.

Heparin Inhibits Cellular Invasion by SARS-CoV-2: Structural Dependence of the Interaction of the Spike S1 Receptor-Binding Domain with Heparin. Thumbnail


Authors

Courtney J. Mycroft-West

Dunhao Su

Isabel Pagani  

Timothy R. Rudd  

Stefano Elli  

Neha S. Gandhi  

Maria C.Z. Meneghetti

Helena B. Nader

Yong Li

Quentin M. Nunes

Nicasio Mancini

Massimo Clementi

Antonella Bisio

Nicholas R. Forsyth

Vito Ferro

Marco Guerrini  

David G. Fernig  

Elisa Vicenzi  

Edwin A. Yates  



Abstract

The dependence of development and homeostasis in animals on the interaction of hundreds of extracellular regulatory proteins with the peri- and extracellular glycosaminoglycan heparan sulfate (HS) is exploited by many microbial pathogens as a means of adherence and invasion. Heparin, a widely used anticoagulant drug, is structurally similar to HS and is a common experimental proxy. Exogenous heparin prevents infection by a range of viruses, including S-associated coronavirus isolate HSR1. Here, we show that heparin inhibits severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) invasion of Vero cells by up to 80% at doses achievable through prophylaxis and, particularly relevant, within the range deliverable by nebulisation. Surface plasmon resonance and circular dichroism spectroscopy demonstrate that heparin and enoxaparin, a low-molecular-weight heparin which is a clinical anticoagulant, bind and induce a conformational change in the spike (S1) protein receptor-binding domain (S1 RBD) of SARS-CoV-2. A library of heparin derivatives and size-defined fragments were used to probe the structural basis of this interaction. Binding to the RBD is more strongly dependent on the presence of 2-O or 6-O sulfate groups than on N-sulfation and a hexasaccharide is the minimum size required for secondary structural changes to be induced in the RBD. It is likely that inhibition of viral infection arises from an overlap between the binding sites of heparin/HS on S1 RBD and that of the angiotensin-converting enzyme 2. The results suggest a route for the rapid development of a first-line therapeutic by repurposing heparin and its derivatives as antiviral agents against SARS-CoV-2 and other members of the Coronaviridae.

Citation

Mycroft-West, C. J., Su, D., Pagani  , I., Rudd  , T. R., Elli  , S., Gandhi  , N. S., …Skidmore   , M. A. (2020). Heparin Inhibits Cellular Invasion by SARS-CoV-2: Structural Dependence of the Interaction of the Spike S1 Receptor-Binding Domain with Heparin. Thrombosis and Haemostasis, 120(12), 1700 - 1715. https://doi.org/10.1055/s-0040-1721319

Journal Article Type Article
Acceptance Date Oct 13, 2020
Online Publication Date Dec 23, 2020
Publication Date Dec 23, 2020
Publicly Available Date Jun 20, 2023
Journal Thrombosis and Haemostasis
Print ISSN 0340-6245
Electronic ISSN 2567-689X
Publisher Schattauer
Peer Reviewed Peer Reviewed
Volume 120
Issue 12
Pages 1700 - 1715
DOI https://doi.org/10.1055/s-0040-1721319
Keywords heparin, coronavirusAnimals, Anticoagulants, Antiviral Agents, Chlorocebus aethiops, Enoxaparin, Heparin, Humans, Molecular Dynamics Simulation, Nebulizers and Vaporizers, Protein Binding, Protein Conformation, Protein Domains, SARS-CoV-2, Spike Glycoprot
Publisher URL https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-0040-1721319

Files







You might also like



Downloadable Citations