Anti-ovarian cancer activities of alkaloids from Triclisia subcordata olive (Menispermecaea)

Uche, F; Li, WW; Richardson, A; Greenhough, TJ

Abstract

This study investigates the anticancer activities of Triclisia subcordata, identifies and elucidates the structure(s) of the bioactive anticancer compound. The evaluation of anticancer activity was done by in vitro sulforhodamine B dye method using A2780, Igrov-1 and Ovcar-8 ovarian cancer cell lines. Trypan Blue exclusion method for cell viability assay was done using haemocytometer with Countess automated cell counter to determine the viability of cells before and after drug treatment. The results show that different fractions of T. subcordata exhibit different level of anticancer activities in A2780, Igrov-1 and Ovcar-8 ovarian cancer cell lines. The crude alkaloid fraction showed highest anticancer activity with IC50 of 1.70 µg/mL while the least activity was observed from fraction rich in phenolic compounds with IC50 of 53.12 µg/mL. Spectroscopic data analysis revealed one of the anticancer bioactive components to be Cycleanine (code as TS3) with IC50 of 7.56µM in A2780 and 44% viability at dose of 10µM. This study could justifies the traditional use of T. subcordata in cancer treatment.

Citation

Uche, F., Li, W., Richardson, A., & Greenhough, T. Anti-ovarian cancer activities of alkaloids from Triclisia subcordata olive (Menispermecaea). . https://doi.org/10.1055/s-0034-1382585

• Conference Name: American Society of Pharmacognosy 2014 Annual Meeting - in conjunction with the 14th Annual Oxford International Conference on the Science of Botanicals
• Conference Location: Oxford, Mississippi, USA
• Start Date: Aug 2, 2014
• End Date: Aug 6, 2014
• Online Publication Date: Jul 14, 2014
• Deposit Date: Jun 8, 2023
• Publisher: Thieme Gruppe
• Volume: 80
• DOI: https://doi.org/10.1055/s-0034-1382585
• Keywords: Organic Chemistry; Complementary and alternative medicine; Drug Discovery; Pharmaceutical Science; Pharmacology; Molecular Medicine; Analytical Chemistry