Single-Molecule Detection of 5-Hydroxymethylcytosine in DNA through Chemical Modification and Nanopore Analysis

Li, Wen-Wu; Gong, Lingzhi; Bayley, Hagan

Abstract

DNA threading the needle: A new method of single-molecule detection of 5-hydroxymethylcytosine (5hmC) in DNA has been developed. Selective thiol substitution of 5hmC (giving SMC) in a single-step, bisulfite-mediated reaction (see scheme) allows the incorporation of a peptide (yellow sphere) or biotin into DNA. Modified 5hmC bases can be readily distinguished at the single-molecule level using protein nanopore analysis.

Citation

Li, W., Gong, L., & Bayley, H. (2013). Single-Molecule Detection of 5-Hydroxymethylcytosine in DNA through Chemical Modification and Nanopore Analysis. Angewandte Chemie International Edition, 52(16), 4350-4355. https://doi.org/10.1002/anie.201300413

• Journal Article Type: Article
• Online Publication Date: Apr 4, 2013
• Publication Date: Apr 15, 2013
• Deposit Date: Jun 13, 2023
• Journal: Angewandte Chemie International Edition
• Print ISSN: 1433-7851
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 52
• Issue: 16
• Pages: 4350-4355
• DOI: https://doi.org/10.1002/anie.201300413
• Keywords: General Chemistry; Catalysis