Natural heterogeneous catalysis with immobilised oxidase biocatalysts

Mattey, Ashley P.; Sangster, Jack J.; Ramsden, Jeremy I.; Baldwin, Christopher; Birmingham, William R.; Heath, Rachel S.; Angelastro, Antonio; Turner, Nicholas J.; Cosgrove, Sebastian C.; Flitsch, Sabine L.

Abstract

The generation of immobilised oxidase biocatalysts allowing multifunctional oxidation of valuable chemicals using molecular oxygen is described. Engineered galactose oxidase (GOase) variants M1 and M3–5, an engineered choline oxidase (AcCO6) and monoamine oxidase (MAO-N D9) displayed long-term stability and reusability over several weeks when covalently attached on a solid support, outperforming their free counterparts in terms of stability (more than 20 fold), resistance to heat at 60 °C, and tolerance to neat organic solvents such as hexane and toluene. These robust heterogenous oxidation catalysts can be recovered after each reaction and be reused multiple times for the oxidation of different substrates.

Citation

Mattey, A. P., Sangster, J. J., Ramsden, J. I., Baldwin, C., Birmingham, W. R., Heath, R. S., …Flitsch, S. L. (in press). Natural heterogeneous catalysis with immobilised oxidase biocatalysts. RSC advances, 10(33), 19501-19505. https://doi.org/10.1039/d0ra03618h

• Journal Article Type: Article
• Acceptance Date: May 18, 2020
• Online Publication Date: May 21, 2020
• Deposit Date: Jun 16, 2023
• Journal: RSC Advances
• Print ISSN: 2046-2069
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Issue: 33
• Pages: 19501-19505
• DOI: https://doi.org/10.1039/d0ra03618h
• Keywords: General Chemical Engineering; General Chemistry
• Publisher URL: https://pubs.rsc.org/en/content/articlelanding/2020/ra/d0ra03618h
• Related Public URLs: https://chemrxiv.org/engage/chemrxiv/article-details/60c74999ee301c0467c79ab9
• Additional Information: : This document is Similarity Check deposited; : Supplementary Information; : Ashley P. Mattey (ORCID); : Christopher Baldwin (ORCID); : William R. Birmingham (ORCID); : Antonio Angelastro (ORCID); : Nicholas J. Turner (ORCID); : Sebastian C. Cosgrove (ORCID); : Sabine L. Flitsch (ORCID); : Single-blind; : Received 7 April 2020; Accepted 18 May 2020; Version of Record published 21 May 2020