Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films

Ahmad, Norlaily; Colak, Burcu; Gibbs, Martin John; Zhang, De-Wen; Becer, C. Remzi; Watkinson, Michael; Gautrot, Julien E.; Krause, Steffi

Abstract

Peptide cross-linked poly(ethylene glycol) hydrogel is a promising biomaterial that has been used widely for drug delivery and tissue engineering. However, the use of this material as a biosensor material for the detection of collagenase has not been explored. Collagenase play a key role in rheumatoid arthritis and osteoarthritis. Detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals and their degradation in the presence of collagenase was monitored using a Quartz Crystal Microbalance (QCM). The biosensor was shown to respond to concentrations between 2 nM to 2000 nM with a lower detection limit of 2 nM.

Citation

Ahmad, N., Colak, B., Gibbs, M. J., Zhang, D.-W., Becer, C. R., Watkinson, M., Gautrot, J. E., & Krause, S. (2018, September). Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films. Presented at Eurosensors 2018 Conference, Graz, Austria

• Presentation Conference Type: Conference Paper (published)
• Conference Name: Eurosensors 2018 Conference
• Start Date: Sep 9, 2018
• End Date: Sep 12, 2018
• Online Publication Date: Nov 30, 2018
• Deposit Date: Jun 16, 2023
• Publisher: MDPI
• Volume: 2
• DOI: https://doi.org/10.3390/proceedings2130961
• Keywords: collagenase biosensor; peptide cross-linked; hydrogel; quartz crystal microbalance; degradation; poly(ethylene glycol)
• Public URL: https://keele-repository.worktribe.com/output/473789