Skip to main content

Research Repository

Advanced Search

Protect to detect: A Golgi apparatus targeted probe to image mobile zinc through the use of a lipophilic cell-labile protecting group strategy

Protect to detect: A Golgi apparatus targeted probe to image mobile zinc through the use of a lipophilic cell-labile protecting group strategy Thumbnail


Abstract

The Golgi apparatus requires zinc for its normal function, but the role it plays in these processes at the sub-cellular level is not well-understood due to the lack of appropriate tools to image it. Herein, a small molecule Golgi apparatus targeted probe was developed to image mobile Zn2+. A trityl group was used to protect a Golgi apparatus targeting cysteine residue to increase cell membrane permeability, which was then removed within 24 h, revealing the free cysteine targeting motif to anchor the probe to the Golgi apparatus. The probe shows good photophysical properties, good selectivity and Zn2+ response over a wide range of pH as well as low cellular toxicity. The probe was shown to be capable of targeting the Golgi apparatus and responding to Zn2+ in a number of different cell lines and was also applied to monitor the change of concentration of mobile Zn2+ in the Golgi apparatus in response to oxidative stress.

Journal Article Type Article
Acceptance Date Mar 21, 2021
Online Publication Date Mar 24, 2021
Publication Date Jul 1, 2021
Publicly Available Date May 30, 2023
Journal Sensors and Actuators B: Chemical
Print ISSN 0925-4005
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 338
Article Number 129850
DOI https://doi.org/10.1016/j.snb.2021.129850
Keywords Golgi apparatus targeting; Fluorescent probe; Zinc imaging; Click chemistry; Oxidative stress
Publisher URL https://www.sciencedirect.com/science/article/pii/S0925400521004196?via%3Dihub

Files







You might also like



Downloadable Citations