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Characterization of miR-200 family members as blood biomarkers for human and laying hen ovarian cancer

Choi, Pui-Wah; Bahrampour, Abbas; Ng, Shu-Kay; Liu, Sze Kei; Qiu, Wei; Xie, Fang; Kuo, Winston Patrick; Kwong, Joseph; Hales, Karen H.; Hales, Dale B.; Wong, Kwong-Kwok; Norwitz, Errol R.; Chow, Chun Kin; Berkowitz, Ross S.; Ng, Shu-Wing

Authors

Pui-Wah Choi

Abbas Bahrampour

Shu-Kay Ng

Sze Kei Liu

Wei Qiu

Fang Xie

Winston Patrick Kuo

Karen H. Hales

Dale B. Hales

Kwong-Kwok Wong

Errol R. Norwitz

Chun Kin Chow

Ross S. Berkowitz

Shu-Wing Ng



Abstract

MicroRNA-200 (miR-200) family is highly expressed in ovarian cancer. We evaluated the levels of family members relative to the internal control miR-103a in ovarian cancer and control blood specimens collected from American and Hong Kong Chinese institutions, as well as from a laying hen spontaneous ovarian cancer model. The levels of miR-200a, miR-200b and miR-200c were significantly elevated in all human cancer versus all control blood samples. Further analyses showed significantly higher miR-200 levels in Chinese control (except miR-429) and cancer (except miR-200a and miR141) samples than their respective American counterparts. Subtype-specific analysis showed that miR-200b had an overall elevated level in serous cancer compared with controls, whereas miR-429 was significantly elevated in clear cell and endometrioid cancer versus controls. MiR-429 was also significantly elevated in cancer versus control in laying hen plasma samples, consistent with the fact that endometrioid tumor is the prevalent type in this species. A neural network model consisting of miR-200a/200b/429/141 showed an area under the curve (AUC) value of 0.904 for American ovarian cancer prediction, whereas a model consisting of miR-200b/200c/429/141 showed an AUC value of 0.901 for Chinese women. Hence, miR-200 is informative as blood biomarkers for both human and laying hen ovarian cancer.

Citation

Choi, P., Bahrampour, A., Ng, S., Liu, S. K., Qiu, W., Xie, F., …Ng, S. (in press). Characterization of miR-200 family members as blood biomarkers for human and laying hen ovarian cancer. Scientific reports, 10(1), https://doi.org/10.1038/s41598-020-77068-0

Journal Article Type Article
Acceptance Date Oct 21, 2020
Online Publication Date Nov 18, 2020
Deposit Date Jun 21, 2023
Journal Scientific Reports
Print ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 10
Issue 1
DOI https://doi.org/10.1038/s41598-020-77068-0
Keywords Multidisciplinary
Additional Information Received: 30 May 2020; Accepted: 21 October 2020; First Online: 18 November 2020; : The authors declare no competing interests.