Extracellular vesicles derived from umbilical cord mesenchymal stromal cells show enhanced anti-inflammatory properties via upregulation of miRNAs after pro-inflammatory priming

Hyland, Mairead; Mennan, Claire; Davies, Rebecca; Wilson, Emma; Tonge, Daniel P; Clayton, Aled; Kehoe, Oksana

Abstract

Autoimmune conditions, such as rheumatoid arthritis, are characterised by a loss of immune tolerance, whereby the immune cells attack self-antigens causing pain and inflammation. These conditions can be brought into remission using pharmaceutical treatments, but often have adverse side effects and some patients do not respond favourably to them. Human umbilical cord mesenchymal stromal cells (UCMSCs) present a promising alternative therapeutic due to their innate anti-inflammatory properties which can be strengthened using pro-inflammatory conditions. Their therapeutic mechanism of action has been attributed to paracrine signalling, by which nanosized acellular particles called 'extracellular vesicles' (EVs) are one of the essential components. Therefore, this research analysed the anti-inflammatory properties of UCMSC-EVs 'primed' with pro-inflammatory cytokines and at baseline with no inflammatory cytokines (control). Both control and primed EVs were co-cultured with un-pooled peripheral blood mononuclear cells (PBMCs; n = 6) from healthy donors. Neither control nor primed EVs exerted a pro-inflammatory effect on PBMCs. Instead, the primed EVs showed the immunosuppressive potential by increasing the expression of the anti-inflammatory protein FoxP3 in PBMCs. This may be attributed to the upregulated miRNAs identified in primed EVs in comparison to control EVs (miR-139-5p, miR-140-5p, miR-214-5p). These findings aid in understanding how UCMSC-EVs mediate immunosuppression and support their potential use in treating autoimmune conditions. [Abstract copyright: © 2023. The Author(s).]

Citation

Hyland, M., Mennan, C., Davies, R., Wilson, E., Tonge, D. P., Clayton, A., & Kehoe, O. (2023). Extracellular vesicles derived from umbilical cord mesenchymal stromal cells show enhanced anti-inflammatory properties via upregulation of miRNAs after pro-inflammatory priming. Stem Cell Reviews and Reports, 19, 2391–2406. https://doi.org/10.1007/s12015-023-10586-2

• Journal Article Type: Article
• Acceptance Date: Jul 3, 2023
• Online Publication Date: Jul 20, 2023
• Publication Date: Jul 20, 2023
• Deposit Date: Aug 1, 2023
• Publicly Available Date: Jan 16, 2024
• Journal: Stem Cell Reviews and Reports
• Print ISSN: 1550-8943
• Electronic ISSN: 2629-3277
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Pages: 2391–2406
• DOI: https://doi.org/10.1007/s12015-023-10586-2
• Keywords: Immunomodulation, Pro-inflammatory priming, Umbilical cord mesenchymal stromal cell, Culture conditions, Extracellular vesicles
• Publisher URL: https://link.springer.com/article/10.1007/s12015-023-10586-2