Functional expression cloning reveals proapoptotic role for protein phosphatase 4

Mourtada-Maarabouni, M; Kirkham, L; Jenkins, B; Rayner, J; Gonda, T J; Starr, R; Trayner, I; Farzaneh, F; Williams, G T

Abstract

Functional expression cloning strategies are highly suitable for the analysis of the molecular control of apoptosis. This approach has two critical advantages. Firstly, it eliminates prior assumptions about the properties of the proteins involved, and, secondly, it selectively targets proteins that are causally involved in apoptosis control and which affect the crucial cellular decision between survival and death. The application of this strategy to the isolation of cDNAs conferring resistance to dexamethasone and γ-irradiation resulted in the isolation of a partial cDNA for the catalytic subunit of protein phosphatase 4 (PP4). Cells transfected with this partial cDNA in an expression vector downregulated PP4 and were resistant to both dexamethasone and UV radiation, as demonstrated by both membrane integrity and colony-forming assays. These observations suggest that PP4 plays an important proapoptotic role in T lymphocytes.

Citation

Mourtada-Maarabouni, M., Kirkham, L., Jenkins, B., Rayner, J., Gonda, T. J., Starr, R., …Williams, G. T. (2003). Functional expression cloning reveals proapoptotic role for protein phosphatase 4. Cell Death and Differentiation, 10, Article 1016–1024. https://doi.org/10.1038/sj.cdd.4401274

• Journal Article Type: Article
• Acceptance Date: Apr 15, 2003
• Online Publication Date: Aug 22, 2003
• Publication Date: Sep 1, 2003
• Deposit Date: May 16, 2024
• Journal: Cell Death and Differentiation
• Print ISSN: 1350-9047
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 10
• Article Number: 1016–1024
• ISBN: 13509047
• DOI: https://doi.org/10.1038/sj.cdd.4401274
• Public URL: https://keele-repository.worktribe.com/output/543809
• Publisher URL: https://www.nature.com/articles/4401274