Daisy Evans
Evaluating the Feasibility of Hydrogel-Based Neural Cell Sprays
Evans, Daisy; Barcons, Aina Mogas; Basit, Raja Haseeb; Adams, Christopher; Chari, Divya Maitreyi
Abstract
Neurological injuries have poor prognoses with serious clinical sequelae. Stem cell transplantation enhances neural repair but is hampered by low graft survival (<ca. 5%), necessitating the development of approaches to enhance post-transplant cell viability. Intracerebral injection exerts high mechanical forces on transplant cells with risks of haemorrhage/infection. Transplant cell sprays can offer a non-invasive alternative. This study has assessed if the addition of protective, encapsulating polymer hydrogels to a cell spray format is feasible. Hydrogels (0.1% (1 mg/mL), 0.3% and 0.6% type I rat tail collagen) were trialled for spray deliverability. Cell-enriched hydrogels (containing mouse cortical astrocytes) were sprayed onto culture substrates. Astrocyte viability, cell-specific marker expression, morphology and proliferation were assessed at 24 h and 72 h post spraying. Intra-gel astrocytes and hydrogels could be co-stained using a double immunocytological technique (picrosirius red (PR)/DAB-peroxidase co-labelling). Astrocyte viability remained high post spraying with hydrogel encapsulation (>ca. 80%) and marker expression/proliferative potential of hydrogel-sprayed astrocytes was retained. Combining a cell spray format with polymer encapsulation technologies could form the basis of a non-invasive graft delivery method, offering potential advantages over current cell delivery approaches.
Citation
Evans, D., Barcons, A. M., Basit, R. H., Adams, C., & Chari, D. M. (in press). Evaluating the Feasibility of Hydrogel-Based Neural Cell Sprays. Journal of Functional Biomaterials, 14(10), Article 527. https://doi.org/10.3390/jfb14100527
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 11, 2023 |
Online Publication Date | Oct 19, 2023 |
Deposit Date | Nov 6, 2023 |
Journal | Journal of Functional Biomaterials |
Print ISSN | 2079-4983 |
Publisher | MDPI |
Peer Reviewed | Peer Reviewed |
Volume | 14 |
Issue | 10 |
Article Number | 527 |
DOI | https://doi.org/10.3390/jfb14100527 |
Keywords | Biomedical Engineering, Biomaterials |
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