Aina Mogas Barcons
Systematic Alignment Analysis of Neural Transplant Cells in Electrospun Nanofibre Scaffolds
Barcons, Aina Mogas; Chowdhury, Farhana; Chari, Divya M; Adams, Christopher
Abstract
<jats:p>Spinal cord injury is debilitating with functional loss often permanent due to a lack of neuro-regenerative or neuro-therapeutic strategies. A promising approach to enhance biological function is through implantation of tissue engineered constructs, to offer neural cell replacement and reconstruction of the functional neuro-architecture. A key goal is to achieve spatially targeted guidance of regenerating tissue across the lesion site to achieve an aligned tissue structure lost as a consequence of injury. Electrospun nanofibres mimic the nanoscale architecture of the spinal cord, can be readily aligned, functionalised with pro-regenerative molecules and incorporated into implantable matrices to provide topographical cues. Crucially, electrospun nanofibers are routinely manufactured at a scale required for clinical use. Although promising, few studies have tested whether electrospun nanofibres can guide targeted spatial growth of clinically relevant neural stem/precursor populations. The alignment fate of daughter cells (derived from the pre-aligned parent cells) has also received limited attention. Further, a standardised quantification methodology to correlate neural cell alignment with topographical cues is not available. We have adapted an image analysis technique to quantify nanofibre-induced alignment of neural cells. Using this method, we show that two key neural stem/precursor populations of clinical relevance (namely, neural stem cells (NSCs) and oligodendrocyte precursor cells), reproducibly orientate their growth to aligned, high-density electrospun nanofiber meshes, but not randomly distributed ones. Daughter populations derived from aligned NSCs (neurons and astrocytes) maintained their alignment following differentiation, but oligodendrocytes did not. Our data show that pre-aligned transplant populations can be used to generate complex, multicellular aligned-fibre constructs for neural implantation.</jats:p>
Citation
Barcons, A. M., Chowdhury, F., Chari, D. M., & Adams, C. (2022). Systematic Alignment Analysis of Neural Transplant Cells in Electrospun Nanofibre Scaffolds. Materials, 16, 124 - 124. https://doi.org/10.3390/ma16010124
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 16, 2022 |
Publication Date | Dec 23, 2022 |
Journal | Materials |
Publisher | MDPI |
Volume | 16 |
Pages | 124 - 124 |
DOI | https://doi.org/10.3390/ma16010124 |
Publisher URL | https://www.mdpi.com/1996-1944/16/1/124 |
Additional Information | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Files
materials-16-00124.pdf
(4.4 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Electroactive Scaffolds to Improve Neural Stem Cell Therapy for Spinal Cord Injury
(2022)
Journal Article
Downloadable Citations
About Keele Repository
Administrator e-mail: research.openaccess@keele.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search