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Eosinophil Chemotaxis

Dent, Gordon

Authors



Abstract

Chemotaxis assays have a number of applications in the study of leukocyte biology and immune/inflammatory pathology. Multiwell “blind chamber”-type assays allow a large number of parallel measurements within a single assay. The development of fluorescence assays using microplate-based chemotaxis chambers has permitted a degree of automation to be applied to these assays. Here, a method is described for the quantitative measurement of eosinophil migration using a 96-well assay with numbers of cells that may realistically be obtained from blood samples.

Online Publication Date Jan 1, 2014
Publication Date Jun 12, 2014
Deposit Date Dec 8, 2023
Pages 101-110
ISBN 9781493910151; 9781493910168
DOI https://doi.org/10.1007/978-1-4939-1016-8_10
Additional Information First Online: 12 June 2014
PROTOCOL PAPER