Synergic production of neutrophil chemotactic activity by colonic epithelial cells and eosinophils

Dent, Gordon; Loweth, Sam C.; Hasan, Anwar Matar; Leslie, Fiona M.

Abstract

The presence of eosinophils in the lumen and mucosa of the intestine is characteristic of both ulcerative colitis (UC) and Crohn's disease (CD). There is evidence of eosinophil activation in the intestine during acute inflammatory episodes of these diseases; these episodes are also characterized by an influx of neutrophils, which have the potential to cause extensive tissue damage. We undertook a study to determine whether eosinophils in contact with colonic epithelial cells produce factors that may attract neutrophils in response to immunological stimulation. Neutrophil chemotactic activity (NCA) and concentrations of three neutrophil-attracting CXC chemokines – CXCL1 (Groα), CXCL5 (Ena78) and CXCL8 (IL8) – were measured in supernatants of T84 colonic epithelial cells and blood eosinophils or eosinophil-like myeloid leukaemia cells (AML14.3D10), alone or in combination. Cells were stimulated with serum-opsonized zymosan (OZ) particles. NCA (P < 0.005) and CXCL5 levels (P < 0.05) in the supernatants of OZ-stimulated epithelial/eosinophil co-cultures were significantly higher than in the supernatants of either cell type alone. Release of CXCL1 (P < 0.05) and CXCL8 (P < 0.01) from OZ-stimulated co-culture supernatants was significantly higher than from OZ-stimulated eosinophils but not higher than from OZ-stimulated epithelial cells. Eosinophils and colonic epithelial cells exhibit synergy in production of neutrophil chemoattractants in response to immunological stimulation. This may represent a mechanism for exaggerated recruitment of neutrophils to the intestine in response to acute infection in conditions that are characterized by the presence of eosinophils in the bowel.

Citation

Dent, G., Loweth, S. C., Hasan, A. M., & Leslie, F. M. (2014). Synergic production of neutrophil chemotactic activity by colonic epithelial cells and eosinophils. Immunobiology, 219(10), 793-797. https://doi.org/10.1016/j.imbio.2014.06.008

• Journal Article Type: Article
• Acceptance Date: Jun 30, 2014
• Online Publication Date: Jul 22, 2014
• Publication Date: 2014-10
• Deposit Date: Dec 8, 2023
• Journal: Immunobiology
• Print ISSN: 0171-2985
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 219
• Issue: 10
• Pages: 793-797
• DOI: https://doi.org/10.1016/j.imbio.2014.06.008
• Keywords: Hematology; Immunology; Immunology and Allergy
• Additional Information: This article is maintained by: Elsevier; Article Title: Synergic production of neutrophil chemotactic activity by colonic epithelial cells and eosinophils; Journal Title: Immunobiology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.imbio.2014.06.008; Content Type: article; Copyright: Copyright © 2014 Elsevier GmbH. All rights reserved.