Characterization of canine intestinal microRNA expression in inflammatory bowel disease and T-cell lymphoma

Irving, Jennifer R.; Hiron, Thomas K.; Davison, Lucy J.; Xia, Dong; Beck, Samuel; Werling, Dirk; Williams, Jonathan

Abstract

Differentiating between canine inflammatory bowel disease (IBD) and intestinal T-cell lymphoma by histopathological examination of endoscopically-derived intestinal biopsies can be challenging and involves an invasive procedure requiring specialized equipment and training. A rapid, non-invasive method of diagnosis, such as blood or faecal analysis for a conserved and stable biomarker, would be a useful adjunct or replacement. Studies on dogs and humans with various types of lymphoma have shown altered microRNA (miRNA) expression patterns in blood, faeces and tissues indicating their potential use as biomarkers of disease. The present study used residual archived endoscopically-derived, formalin-fixed, paraffin-embedded (FFPE) duodenal tissue taken from pet dogs undergoing routine investigation of gastrointestinal disease. The dogs had previously been diagnosed with either normal/minimal intestinal inflammation, severe IBD or intestinal T-cell lymphoma. Next generation sequencing with qPCR validation was used to elucidate differentially expressed miRNAs between groups. Our results show that miRNA can be extracted from archived endoscopically-derived FFPE tissues from the canine duodenum and used to differentiate normal/minimally inflamed canine duodenal tissue from severe lymphoplasmacytic IBD and T-cell lymphoma.

Citation

Irving, J. R., Hiron, T. K., Davison, L. J., Xia, D., Beck, S., Werling, D., & Williams, J. (2023). Characterization of canine intestinal microRNA expression in inflammatory bowel disease and T-cell lymphoma. Journal of Comparative Pathology, 204, 23-29. https://doi.org/10.1016/j.jcpa.2023.03.186

• Journal Article Type: Article
• Acceptance Date: Mar 30, 2023
• Online Publication Date: Jun 15, 2023
• Publication Date: 2023-07
• Deposit Date: Jan 12, 2024
• Journal: Journal of Comparative Pathology
• Print ISSN: 0021-9975
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 204
• Pages: 23-29
• DOI: https://doi.org/10.1016/j.jcpa.2023.03.186
• Keywords: General Veterinary; Pathology and Forensic Medicine
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0021997523002128?via%3Dihub
• Additional Information: This article is maintained by: Elsevier; Article Title: Characterization of canine intestinal microRNA expression in inflammatory bowel disease and T-cell lymphoma; Journal Title: Journal of Comparative Pathology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jcpa.2023.03.186; Content Type: article; Copyright: © 2023 The Authors. Published by Elsevier Ltd.