Enhancement of Magnetic Nanoparticle-Mediated Gene Transfer to Astrocytes by ‘Magnetofection‘: Effects of Static and Oscillating Fields

Pickard, Mark; Chari, Divya

Abstract

Aims: To assess the feasibility of using magnetic nanoparticles (MNPs) to transfect astrocytes derived for transplantation and determine if transfection efficacy can be enhanced by static and oscillating magnetic fields. Methods: Astrocytes were transfected using MNPs functionalized with a plasmid encoding a reporter protein. Transfection efficacies were compared following application of static fields and a novel, oscillating array system at a range of frequencies. The transplantation potential of transfected cells was tested in organotypic cerebellar slice cultures. Results: Rat astrocytes can be efficiently transfected using MNPs with applied static/oscillating fields; the latter effect is frequency dependant. Transfected astrocytes could survive and differentiate following introduction into 3D neural tissue arrays. Conclusion: MNP vectors can safely and effectively transfect rodent astrocytes and could form the basis of a ‘multifunctional nanoplatform’ for neural cell transplantation.

Citation

Pickard, M., & Chari, D. (2010). Enhancement of Magnetic Nanoparticle-Mediated Gene Transfer to Astrocytes by ‘Magnetofection‘: Effects of Static and Oscillating Fields. Nanomedicine, 5(2), 217-232. https://doi.org/10.2217/nnm.09.109

• Journal Article Type: Article
• Online Publication Date: Feb 11, 2010
• Publication Date: 2010-02
• Deposit Date: Jun 11, 2024
• Journal: Nanomedicine
• Print ISSN: 1743-5889
• Electronic ISSN: 1748-6963
• Publisher: Future Medicine
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 2
• Pages: 217-232
• DOI: https://doi.org/10.2217/nnm.09.109
• Public URL: https://keele-repository.worktribe.com/output/848877
• Publisher URL: https://www.tandfonline.com/doi/full/10.2217/nnm.09.109