Interlaboratory study on real-time PCR detection and quantification of the European anglerfish, pike, and seabream parvalbumin gene

Zdeňková, Kamila; Mukherjee, Subham; Marin, Marco A. Lopez; Horká, Petra; Kýrová, Veronika; Potůčková, Miroslava; Čermáková, Eliška

Abstract

This study presents a large-scale interlaboratory comparison (ILC) aimed at detecting and quantifying DNA from two European anglerfish (Lophius budegassa, Lophius piscatorius), pike (Esox lucius) and sea bream (Spondyliosoma cantharus) using real-time qPCR. To detect amplification of the parvalbumin genetic marker, single and multiplex qPCR assays using EvaGreen® dye or TaqMan™ probes were used. Genomic DNA isolated from target fish species and an advanced DNA calibrator, gBlocks® gene fragments, were used as standards. The DNA of anglerfish, pike and sea bream as well as their mixtures were analysed together with 14 other non-target fish species. All target fish samples were correctly identified by the participating laboratories. Qualitative assessment of anglerfish and seabream DNA showed an accuracy rate of 100%, while pike DNA achieved a match rate of 99%. Validation of quantitative protocols in four different laboratories consistently achieved z-scores below 2, indicating satisfactory performance and confirming the high degree of similarity of laboratory results. Furthermore, high accuracy and efficiency were demonstrated for the quantification of anglerfish and seabream DNA by triplex qPCR using TaqMan™ probes. Regarding the selected gene marker, the major fish allergenic protein parvalbumin enables indirect detection and quantification of the allergen in the sample. Therefore, the use of proposed protocols can significantly contribute to protecting the health of consumers and to controlling the food market.

Citation

Zdeňková, K., Mukherjee, S., Marin, M. A. L., Horká, P., Kýrová, V., Potůčková, M., & Čermáková, E. (2024). Interlaboratory study on real-time PCR detection and quantification of the European anglerfish, pike, and seabream parvalbumin gene. European Food Research and Technology, 250(11), 2821-2835. https://doi.org/10.1007/s00217-024-04578-w

• Journal Article Type: Article
• Acceptance Date: May 11, 2024
• Online Publication Date: May 21, 2024
• Publication Date: Nov 1, 2024
• Deposit Date: Jul 9, 2024
• Publicly Available Date: Oct 16, 2024
• Journal: European Food Research and Technology
• Print ISSN: 1438-2377
• Electronic ISSN: 1438-2385
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 250
• Issue: 11
• Pages: 2821-2835
• DOI: https://doi.org/10.1007/s00217-024-04578-w
• Keywords: qPCR, Lophius, Spondyliosoma, Esox, Parvalbumin, DNA calibrator
• Public URL: https://keele-repository.worktribe.com/output/874036
• Publisher URL: https://link.springer.com/article/10.1007/s00217-024-04578-w
• Additional Information: Received: 1 March 2024; Revised: 11 May 2024; Accepted: 11 May 2024; First Online: 21 May 2024; : ; : The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.; : All applicable international, national, and institutional guidelines for the use of animals were followed. The study was conducted following the local legislation and institutional requirements.