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Fluorinating the Sugar and the Nucleotide: Exploring Fluorination Within GDP-Mannose Probes Using Chemoenzymatic Synthesis (2025)
Journal Article
Dolan, J. P., Evans, S. T., Benckendorff, C. M. M., Sari, S., Ní Cheallaigh, A., & Miller, G. J. (2025). Fluorinating the Sugar and the Nucleotide: Exploring Fluorination Within GDP-Mannose Probes Using Chemoenzymatic Synthesis. JACS Au, jacsau.5c00626. https://doi.org/10.1021/jacsau.5c00626

Fluorinated glycans offer a prime opportunity to study the intricacies of their associated binding events with proteins, invoke resistance toward enzymatic hydrolysis, and modulate carbohydrate physicochemical properties. Sugar nucleotides are the ke... Read More about Fluorinating the Sugar and the Nucleotide: Exploring Fluorination Within GDP-Mannose Probes Using Chemoenzymatic Synthesis.

Molecularly Defined Glycocalyx Models Reveal AB5 Toxins Recognize Their Target Glycans Superselectively (2025)
Journal Article
Saltor Núñez, L., Kumar, V., Ross, J. F., Dolan, J. P., Srimasorn, S., Zhang, X., Richter, R. P., & Turnbull, W. B. (2025). Molecularly Defined Glycocalyx Models Reveal AB5 Toxins Recognize Their Target Glycans Superselectively. JACS Au, https://doi.org/10.1021/jacsau.5c00305

AB5 toxins are a class of bacterial toxins that recognize cell surface carbohydrates to facilitate their uptake by the target cell. Among them are cholera toxin (CT) from Vibrio cholerae that causes cholera, and Shiga toxin (STx) from Shigella dysent... Read More about Molecularly Defined Glycocalyx Models Reveal AB5 Toxins Recognize Their Target Glycans Superselectively.

A modular, reusable biocatalytic flow system for UDP-GlcNAc production (2025)
Journal Article
Roberts, T. L., Dolan, J. P., Miller, G. J., Lima, M. A. D., & Cosgrove, S. C. (2025). A modular, reusable biocatalytic flow system for UDP-GlcNAc production. Reaction Chemistry and Engineering, https://doi.org/10.1039/d5re00127g

We report here the continuous flow synthesis of a high-value sugar nucleotide. Immobilisation of enzymes onto solid carriers permitted transfer of the biocatalysts into packed bed reactors to realise a continuous biocatalytic platform for the synthes... Read More about A modular, reusable biocatalytic flow system for UDP-GlcNAc production.

3′-O-β-Glucosylation of nucleoside analogues using a promiscuous bacterial glycosyltransferase (2025)
Journal Article
Dolan, J. P., Keenan, T., Cheallaigh, A. N., Fascione, M. A., & Miller, G. J. (2025). 3′-O-β-Glucosylation of nucleoside analogues using a promiscuous bacterial glycosyltransferase. RSC Chemical Biology, https://doi.org/10.1039/D5CB00026B

Nucleoside analogue therapeutics have a proven capability within drug discovery as antiviral and antineoplastic agents. However, their efficacy can be limited by poor cellular uptake, off target toxicity and low bioavailability. Glycosylation of phar... Read More about 3′-O-β-Glucosylation of nucleoside analogues using a promiscuous bacterial glycosyltransferase.

Correction: Virtual screening, identification and in vitro validation of small molecule GDP-mannose dehydrogenase inhibitors (2024)
Journal Article
Dolan, J. P., Ahmadipour, S., Wahart, A. J. C., Cheallaigh, A. N., Sari, S., Eurtivong, C., Lima, M. A., Skidmore, M. A., Volcho, K. P., Reynisson, J., Field, R. A., & Miller, G. J. (in press). Correction: Virtual screening, identification and in vitro validation of small molecule GDP-mannose dehydrogenase inhibitors. RSC Chemical Biology, https://doi.org/10.1039/d4cb90026j

Correction for ‘Virtual screening, identification and in vitro validation of small molecule GDP-mannose dehydrogenase inhibitors’ by Jonathan P. Dolan et al., RSC Chem. Biol., 2023, 4, 865–870, https://doi.org/10.1039/D3CB00126A.

Cover Feature: Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides (ChemBioChem 1/2024) (2024)
Digital Artefact
Wahart, A. J. C., Dolan, J. P., Anderson, S. D., Ní Cheallaigh, A., Staniland, J., Lima, M. A., Skidmore, M. A., Miller, G. J., & Cosgrove, S. C. (2024). Cover Feature: Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides (ChemBioChem 1/2024). [Cover image]

Wahart et al. demonstrate the use of an immobilised oxidase enzyme coupled to an extractive amino resin for the bioremediation of a common industrial surfactant. This allows the facile purification of alkyl monoglucosides without the need for high en... Read More about Cover Feature: Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides (ChemBioChem 1/2024).

Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a d ‐Aminopeptidase (2023)
Journal Article
Arnott, Z. L. P., Morgan, H. E., Hollingsworth, K., Stevenson, C. M. E., Collins, L. J., Tamasanu, A., Machin, D. C., Dolan, J. P., Kamiński, T. P., Wildsmith, G. C., Williamson, D. J., Pickles, I. B., Warriner, S. L., Turnbull, W. B., & Webb, M. E. (2024). Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a d ‐Aminopeptidase. Angewandte Chemie International Edition, 63(8), Article e202310862. https://doi.org/10.1002/anie.202310862

Quantitative and selective labelling of proteins is widely used in both academic and industrial laboratories, and catalytic labelling of proteins using transpeptidases, such as sortases, has proved to be a popular strategy for such selective modifica... Read More about Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a d ‐Aminopeptidase.

Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a d ‐Aminopeptidase (2023)
Journal Article
Arnott, Z. L. P., Morgan, H. E., Hollingsworth, K., Stevenson, C. M. E., Collins, L. J., Tamasanu, A., Machin, D. C., Dolan, J. P., Kamiński, T. P., Wildsmith, G. C., Williamson, D. J., Pickles, I. B., Warriner, S. L., Turnbull, W. B., & Webb, M. E. (2024). Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a d ‐Aminopeptidase. Angewandte Chemie, Article e202310862. https://doi.org/10.1002/ange.202310862

Quantitative and selective labelling of proteins is widely used in both academic and industrial laboratories, and catalytic labelling of proteins using transpeptidases, such as sortases, has proved to be a popular strategy for such selective modifica... Read More about Quantitative N‐ or C‐Terminal Labelling of Proteins with Unactivated Peptides by Use of Sortases and a d ‐Aminopeptidase.

Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides (2023)
Journal Article
Wahart, A. J. C., Dolan, J. P., Anderson, S. D., Cheallaigh, A. N., Staniland, J., Lima, M. A., Skidmore, M. A., Miller, G. J., & Cosgrove, S. C. (2023). Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides. ChemBioChem, Article e202300752. https://doi.org/10.1002/cbic.202300625

Wahart et al. demonstrate the use of an immobilised oxidase enzyme coupled to an extractive amino resin for the bioremediation of a common industrial surfactant. This allows the facile purification of alkyl monoglucosides without the need for high en... Read More about Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides.

Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides. (2023)
Journal Article
Wahart, A. J. C., Dolan, J. P., Anderson, S. D., Cheallaigh, A. N., Staniland, J., Lima, M. A., Skidmore, M. A., Miller, G. J., & Cosgrove, S. C. (in press). Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides. ChemBioChem, Article e202300625. https://doi.org/10.1002/cbic.202300625

As the world moves towards net-zero carbon emissions, the development of sustainable chemical manufacturing processes is essential. Within manufacturing, purification by distillation is often used, however this process is energy intensive and methods... Read More about Harnessing a Biocatalyst to Bioremediate the Purification of Alkylglycosides..

Virtual screening, identification and in vitro validation of small molecule GDP-mannose dehydrogenase inhibitors † (2023)
Journal Article
Dolan, J., Ahmadipour, S., Wahart, A., Ni Cheallaigh, A., Sari, S., Eurtivong,, C., Lima, M., Skidmore, M., Volcho, K., Reynisson, J., Field, R., & Miller, G. (2023). Virtual screening, identification and in vitro validation of small molecule GDP-mannose dehydrogenase inhibitors †. RSC Chemical Biology, 4(11), 865-870. https://doi.org/10.1039/D3CB00126A

Upon undergoing mucoid conversion within the lungs of cystic fibrosis patients, the pathogenic bacterium Pseudomonas aeruginosa synthesises copious quantities of the virulence factor and exopolysaccharide alginate. The enzyme guanosine diphosphate ma... Read More about Virtual screening, identification and in vitro validation of small molecule GDP-mannose dehydrogenase inhibitors †.

Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis (2022)
Journal Article
Cosgrove, Miller, & Dolan, J. (2022). Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis. JACS Au, 47-61. https://doi.org/10.1021/jacsau.2c00529

While the field of biocatalysis has bloomed over the past 20–30 years, advances in the understanding and improvement of carbohydrate-active enzymes, in particular, the sugar nucleotides involved in glycan building block biosynthesis, have progressed... Read More about Biocatalytic Approaches to Building Blocks for Enzymatic and Chemical Glycan Synthesis.

Synthesis of C6-modified mannose 1-phosphates and evaluation of derived sugar nucleotides against GDP-mannose dehydrogenase. (2022)
Journal Article
Ahmadipour, S., Wahart, A., Dolan, J., Beswick, L., Hawes, C., Field, R., & Miller, G. (2022). Synthesis of C6-modified mannose 1-phosphates and evaluation of derived sugar nucleotides against GDP-mannose dehydrogenase. Beilstein Journal of Organic Chemistry, 18, 1379 - 1384. https://doi.org/10.3762/bjoc.18.142

Sufferers of cystic fibrosis are at significant risk of contracting chronic bacterial lung infections. The dominant pathogen in these cases is mucoid Pseudomonas aeruginosa. Such infections are characterised by overproduction of the exopolysaccharide... Read More about Synthesis of C6-modified mannose 1-phosphates and evaluation of derived sugar nucleotides against GDP-mannose dehydrogenase..

Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions (2022)
Journal Article
P. Dolan, J., Machin, D. C., Dedola, S., Field2, R. A., Webb, M. E., & Bruce Turnbull, W. (2022). Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions. Frontiers in Chemistry, https://doi.org/10.3389/fchem.2022.958272

<jats:p>The chemoenzymatic synthesis of a series of dual N- and C-terminal–functionalized cholera toxin B subunit (CTB) glycoconjugates is described. Mucin 1 peptides bearing different levels of Tn antigen glycosylation [MUC1(Tn)] were prepared <jats... Read More about Synthesis of cholera toxin B subunit glycoconjugates using site-specific orthogonal oxime and sortase ligation reactions.

Bioorthogonal, Bifunctional Linker for Engineering Synthetic Glycoproteins (2022)
Journal Article
McBerney, R., Dolan, J., E. Cawood, E., E. Webb, M., & Bruce Turnbull, W. (2022). Bioorthogonal, Bifunctional Linker for Engineering Synthetic Glycoproteins. JACS Au, https://doi.org/10.1021/jacsau.2c00312

Post-translational glycosylation of proteins results in complex mixtures of heterogeneous protein glycoforms. Glycoproteins have many potential applications from fundamental studies of glycobiology to potential therapeutics, but generating homogeneou... Read More about Bioorthogonal, Bifunctional Linker for Engineering Synthetic Glycoproteins.

Chemical and enzymatic synthesis of the alginate sugar nucleotide building block: GDP-d-mannuronic acid (2019)
Journal Article
Beswick, L., Ahmadipour, S., Dolan, J. P., Rejzek, M., Field, R. A., & Miller, G. J. (2019). Chemical and enzymatic synthesis of the alginate sugar nucleotide building block: GDP-d-mannuronic acid. Carbohydrate Research, 485(1), 107819 -107819. https://doi.org/10.1016/j.carres.2019.107819

Highlights

Sugarnucleotide synthesis.

Chemical and enzymatic access to GDP-d-ManA.

Evaluation of protected and free uronate 1-phosphates for pyrophosphorylation.

Continuous flow iodination using an automated computer-vision controlled liquid-liquid extraction system (2017)
Journal Article
O'Brien, M., Cooper, D., & Dolan, J. (2017). Continuous flow iodination using an automated computer-vision controlled liquid-liquid extraction system. Tetrahedron Letters, 829 - 834. https://doi.org/10.1016/j.tetlet.2017.01.029

Abstract A dynamic computer-vision control system incorporating open-source software technologies (Python, OpenCV) was used to automate a gravity based in-line liquid-liquid extraction during the iodination of enaminones in continuous flow. The syste... Read More about Continuous flow iodination using an automated computer-vision controlled liquid-liquid extraction system.