All Outputs (20)

Tuning the properties of all natural polymeric scaffolds for tendon repair with cellulose microfibers (2024)
Journal Article
Diaz, F., Zimmermann, L., Dale, T. P., Forsyth, N. R., & Boccaccini, A. R. (2024). Tuning the properties of all natural polymeric scaffolds for tendon repair with cellulose microfibers. Carbohydrate Polymer Technologies and Applications, 7, Article 100447. https://doi.org/10.1016/j.carpta.2024.100447

Rotator cuff injuries affect a large percentage of the population worldwide. Surgical repair has rates of failure of up to 70%, and existing materials used in the reinforcement of injuries often lack appropriate mechanical properties and biodegradabi... Read More about Tuning the properties of all natural polymeric scaffolds for tendon repair with cellulose microfibers.

Injectable hydrogels: An emerging therapeutic strategy for cartilage regeneration. (2023)
Journal Article
Atwal, A., Dale, T. P., Snow, M., Forsyth, N. R., & Davoodi, P. (2023). Injectable hydrogels: An emerging therapeutic strategy for cartilage regeneration. Advances in colloid and interface science, 321, Article 103030. https://doi.org/10.1016/j.cis.2023.103030

The impairment of articular cartilage due to traumatic incidents or osteoarthritis has posed significant challenges for healthcare practitioners, researchers, and individuals suffering from these conditions. Due to the absence of an approved treatmen... Read More about Injectable hydrogels: An emerging therapeutic strategy for cartilage regeneration..

Endotenon-Derived Type II Tendon Stem Cells Have Enhanced Proliferative and Tenogenic Potential (2023)
Journal Article
Clerici, M., Citro, V., Byrne, A. L., P. Dale, T., Boccaccini, A. R., Della Porta, G., …Forsyth, N. R. (2023). Endotenon-Derived Type II Tendon Stem Cells Have Enhanced Proliferative and Tenogenic Potential. International Journal of Molecular Sciences, 24(20), Article 15107. https://doi.org/10.3390/ijms242015107

Tendon injuries caused by overuse or age-related deterioration are frequent. Incomplete knowledge of somatic tendon cell biology and their progenitors has hindered interventions for the effective repair of injured tendons. Here, we sought to compare... Read More about Endotenon-Derived Type II Tendon Stem Cells Have Enhanced Proliferative and Tenogenic Potential.

Decellularisation and characterisation of porcine pleura for lung tissue engineering (2023)
Journal Article
Vikranth, T., Dale, T., & Forsyth, N. R. (in press). Decellularisation and characterisation of porcine pleura for lung tissue engineering. bioRxiv, https://doi.org/10.1101/2023.06.20.545830

Decellularisation offers a broad range of biomimetic scaffolds of allogeneic and xenogeneic origins, exhibiting innate tissue-specific characteristics. We explored a physico-chemical method for decellularising porcine pleural membranes (PPM) as poten... Read More about Decellularisation and characterisation of porcine pleura for lung tissue engineering.

Hypoxic conditions promote a proliferative, poorly differentiated phenotype in COPD lung tissue progenitor cells in vitro (2023)
Journal Article
Dale, T. P., Santer, M. D., Haris, M., Zuo, W., & Forsyth, N. R. (2023). Hypoxic conditions promote a proliferative, poorly differentiated phenotype in COPD lung tissue progenitor cells in vitro. Experimental Lung Research, 49(1), 12-26. https://doi.org/10.1080/01902148.2022.2158404

PURPOSE: Chronic obstructive pulmonary disease (COPD) patients experience hypoxemia and lung tissue hypoxia, causing vasoconstriction, and at its most severe Cor pulmonale. However, minimal attention has been given to the effects of hypoxia at the ce... Read More about Hypoxic conditions promote a proliferative, poorly differentiated phenotype in COPD lung tissue progenitor cells in vitro.

3D in-vitro cultures of human bone marrow and Wharton's jelly derived mesenchymal stromal cells show high chondrogenic potential. (2022)
Journal Article
Dale. (2022). 3D in-vitro cultures of human bone marrow and Wharton's jelly derived mesenchymal stromal cells show high chondrogenic potential. Frontiers in Bioengineering and Biotechnology, -. https://doi.org/10.3389/fbioe.2022.986310

In this study, chondrogenic potentials of 3D high-density cultures of Bone Marrow (BM) and Wharton's Jelly (WJ)-derived mesenchymal stromal cells (MSCs) was investigated by chondrogenesis- and cytokine-related gene expression over a 16-day culture pe... Read More about 3D in-vitro cultures of human bone marrow and Wharton's jelly derived mesenchymal stromal cells show high chondrogenic potential..

SUCCESSFUL ISOLATION AND CULTURE OF MULTIPOTENTIAL DISTAL AIRWAY STEM CELLS FROM COPD PATIENTS (2022)
Journal Article
Dale. (2022). SUCCESSFUL ISOLATION AND CULTURE OF MULTIPOTENTIAL DISTAL AIRWAY STEM CELLS FROM COPD PATIENTS. Tissue engineering. Part A, S451 - S452

Chronic Obstructive Pulmonary Disease (COPD) presents a significant, incurable, worldwide health burden, identifying it as a prospective regenerative medicine target. Sourcing cells for lung therapies is challenging; progress has been made in culturi... Read More about SUCCESSFUL ISOLATION AND CULTURE OF MULTIPOTENTIAL DISTAL AIRWAY STEM CELLS FROM COPD PATIENTS.

Physiological Oxygen Causes the Release of Volatile Organic Compounds from Human Pluripotent Stem Cells with Possible Roles in Maintaining Self-Renewal and Pluripotency (2022)
Journal Article
Barreto, S., Al-Zubaidi, M., Dale, T., Worrall, A., Kapacee, Z., Kimber, S., …Rutter, A. (2022). Physiological Oxygen Causes the Release of Volatile Organic Compounds from Human Pluripotent Stem Cells with Possible Roles in Maintaining Self-Renewal and Pluripotency. Preprints, https://doi.org/10.20944/preprints202203.0143.v1

Human pluripotent stem cells (hPSCs) have widespread potential biomedical applications. There is a need for large-scale in vitro production of hPSCs, and optimal culture methods are vital in achieving this. Physiological oxygen (2% O2) improves key h... Read More about Physiological Oxygen Causes the Release of Volatile Organic Compounds from Human Pluripotent Stem Cells with Possible Roles in Maintaining Self-Renewal and Pluripotency.

3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells. (2021)
Journal Article
Camilla Ciardulli, M., Lovecchio, J., Scala, P., Pavel Lamparelli, E., Dale, T., Giudice, V., …Della Porta, G. (2021). 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells. Pharmaceutics, 13(9), https://doi.org/10.3390/pharmaceutics13091448

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton's Jelly (hWJ-MSCs). The 3D-biomimetic and bi... Read More about 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells..

Chondrogenic Commitment of Human Bone Marrow Mesenchymal Stem Cells in a Perfused Collagen Hydrogel Functionalized with hTGF-ß1-Releasing PLGA Microcarrier (2021)
Journal Article
Dale, T., Forsyth, N., & Maffulli, N. (2021). Chondrogenic Commitment of Human Bone Marrow Mesenchymal Stem Cells in a Perfused Collagen Hydrogel Functionalized with hTGF-ß1-Releasing PLGA Microcarrier. Pharmaceutics,

Tissue engineering strategies can be relevant for cartilage repair and regeneration. A collagen matrix was functionalized with the addition of poly-lactic-co-glycolic acid microcarriers (PLGA-MCs) carrying a human Transforming Growth Factor ß1 (hTFG-... Read More about Chondrogenic Commitment of Human Bone Marrow Mesenchymal Stem Cells in a Perfused Collagen Hydrogel Functionalized with hTGF-ß1-Releasing PLGA Microcarrier.

Genetically engineered distal airway stem cell transplantation protects mice from pulmonary infection (2020)
Journal Article
Dale, T., & Forsyth, N. (2020). Genetically engineered distal airway stem cell transplantation protects mice from pulmonary infection. EMBO Molecular Medicine, https://doi.org/10.15252/emmm.201810233

Severe pulmonary infection is a major threat to human health accompanied by substantial, which increases medical costs, prolonged inpatient requirements, and high mortality rates. New anti-microbial therapeutic strategies are urgently required to add... Read More about Genetically engineered distal airway stem cell transplantation protects mice from pulmonary infection.

Rock Inhibitor Y-27632 Enables Feeder-Free, Unlimited Expansion of Sus scrofa domesticus Swine Airway Stem Cells to Facilitate Respiratory Research (2019)
Journal Article
Dale, T. P., Borg D’anastasi, E., Haris, M., & Forsyth, N. R. (2019). Rock Inhibitor Y-27632 Enables Feeder-Free, Unlimited Expansion of Sus scrofa domesticus Swine Airway Stem Cells to Facilitate Respiratory Research. Stem Cells International, 2019, 1 - 15. https://doi.org/10.1155/2019/3010656

Current limitations in the efficacy of treatments for chronic respiratory disorders position them as prospective regenerative medicine therapeutic targets. A substantial barrier to these ambitions is that research requires large numbers of cells whos... Read More about Rock Inhibitor Y-27632 Enables Feeder-Free, Unlimited Expansion of Sus scrofa domesticus Swine Airway Stem Cells to Facilitate Respiratory Research.

Hypoxia-modified cancer cell metabolism (2019)
Journal Article
Al Tameemi, W., Dale, T. P., Kh Al-Jumaily, R. M., & Forsyth, N. R. (2019). Hypoxia-modified cancer cell metabolism. Frontiers in Cell and Developmental Biology, 7(4), https://doi.org/10.3389/fcell.2019.00004

While oxygen is critical to the continued existence of complex organisms, extreme levels of oxygen within a system, known as hypoxia (low levels of oxygen) and hyperoxia (excessive levels of oxygen), potentially promote stress within a defined biolog... Read More about Hypoxia-modified cancer cell metabolism.

Ectopic Telomerase Expression Fails to Maintain Chondrogenic Capacity in Three-Dimensional Cultures of Clinically Relevant Cell Types. (2018)
Journal Article
Dale, T. P., & Forsyth, N. R. (in press). Ectopic Telomerase Expression Fails to Maintain Chondrogenic Capacity in Three-Dimensional Cultures of Clinically Relevant Cell Types. BioResearch Open Access, 7(1), 10 - 24. https://doi.org/10.1089/biores.2018.0008

The poor healing capacity of cartilage and lack of effective treatment for associated disease and trauma makes it a strong candidate for a regenerative medicine approach. Potential therapies tested to date, although effective, have met with a number... Read More about Ectopic Telomerase Expression Fails to Maintain Chondrogenic Capacity in Three-Dimensional Cultures of Clinically Relevant Cell Types..

Tenogenic differentiation of human embryonic stem cells (2018)
Journal Article
Dale, T. P., Mazher, S., Webb, W. R., Zhou, J., Maffulli, N., Chen, G., …Forsyth, N. R. (2018). Tenogenic differentiation of human embryonic stem cells. Tissue engineering. Part A, 24(5-6), 361-368. https://doi.org/10.1089/ten.TEA.2017.0017

Tendon healing is complex to manage because of the limited regeneration capacity of tendon tissue; stem cell-based tissue engineering approaches may provide alternative healing strategies. We sought to determine whether human embryonic stem cells (hE... Read More about Tenogenic differentiation of human embryonic stem cells.

Pluripotent and Multipotent Stem Cells Display Distinct Hypoxic miRNA Expression Profiles (2016)
Journal Article
Agrawal, R., Dale, T. P., Al-Zubaidi, M. A., Benny Malgulwar, P., Forsyth, N. R., & Kulshreshtha, R. (in press). Pluripotent and Multipotent Stem Cells Display Distinct Hypoxic miRNA Expression Profiles. PloS one, 11(10), e0164976. https://doi.org/10.1371/journal.pone.0164976

MicroRNAs are reported to have a crucial role in the regulation of self-renewal and differentiation of stem cells. Hypoxia has been identified as a key biophysical element of the stem cell culture milieu however, the link between hypoxia and miRNA ex... Read More about Pluripotent and Multipotent Stem Cells Display Distinct Hypoxic miRNA Expression Profiles.

Self-renewal of human embryonic stem cells on defined synthetic electrospun nanofibers (2015)
Journal Article
Kumar, D., Dale, T. P., Yang, Y., & Forsyth, N. R. (2015). Self-renewal of human embryonic stem cells on defined synthetic electrospun nanofibers. Biomedical Materials, 10(6), Article 065017. https://doi.org/10.1088/1748-6041/10/6/065017

Human embryonic stem cells (hESCs) are conventionally expanded and maintained in vitro on biological substrates. Synthetic electrospun polymer nanofibers have the potential to act as non-biological substrates in the culture of hESCs. Three synthetic,... Read More about Self-renewal of human embryonic stem cells on defined synthetic electrospun nanofibers.

Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner. (2015)
Journal Article
Dale, T. P., de Castro, A., Kuiper, N. J., Parkinson, E. K., & Forsyth, N. R. (2015). Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner. PloS one, 10(7), Article e0133745. https://doi.org/10.1371/journal.pone.0133745

BACKGROUND: Limited options for the treatment of cartilage damage have driven the development of tissue engineered or cell therapy alternatives reliant on ex vivo cell expansion. The study of chondrogenesis in primary cells is difficult due to progre... Read More about Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner..

BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation. (2015)
Journal Article
Gawain Kay, A., Dale, T., Mehedi Akram, K., Mohan, P., Hampson, K., Maffulli, N., …Forsyth, N. (2015). BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation. Regenerative Medicine, 10(2), 109 - 125. https://doi.org/10.2217/rme.14.67

AIM: Human mesenchymal stem cells (hMSC) are multipotent progenitor cells. We propose the optimization of hMSC isolation and recovery using the application of a controlled hypoxic environment. MATERIALS & METHODS: We evaluated oxygen, glucose and ser... Read More about BMP2 repression and optimized culture conditions promote human bone marrow-derived mesenchymal stem cell isolation..

The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model (2013)
Journal Article
Webb, W. R., Dale, T. P., Lomas, A. J., Zeng, G., Wimpenny, I., El Haj, A. J., …Chen, G. (2013). The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model. Biomaterials, 34(28), 6683-6694. https://doi.org/10.1016/j.biomaterials.2013.05.041

Tendon injuries and defects present a substantial burden to global healthcare economies. There are no synthetic/biosynthesised implants available which can restore full function or match the mechanical properties of native tendon. Therefore, poly(3-h... Read More about The application of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds for tendon repair in the rat model.