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All Outputs (29)

Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study (2024)
Journal Article
Wilkinson, H., McDonald, J., McCarthy, H., Perry, J., Wright, K., Hulme, C., & Cool, P. (in press). Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study. Briefings in Functional Genomics, Article elae008. https://doi.org/10.1093/bfgp/elae008

This project investigates if third-generation genomic sequencing can be used to identify the species of bacteria causing prosthetic joint infections (PJIs) at the time of revision surgery. Samples of prosthetic fluid were taken during revision surger... Read More about Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study.

CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS (2023)
Journal Article
Lan, T., Wright, K., Makwana, N., Bing, A., McCarthy, H., & Hulme, C. (2023). CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS. Orthopaedic Proceedings, 105-B(SUPP_16), 62-62. https://doi.org/10.1302/1358-992x.2023.16.062

AbstractObjectivesBone marrow aspirate concentrate (BMAC), together with fibrin glue (Tisseel, Baxter, UK) and Hyaluronic acid (HA) were used as a one-step cell therapy treating patients with ankle cartilage defects in our hospital. This therapy was... Read More about CHARACTERIZING CELLULAR COMPONENTS OF BONE MARROW ASPIRATE CONCENTRATE: A ONE-STEP CELL THERAPY FOR ANKLE CARTILAGE DEFECTS.

Comparison of Knee Articular Cartilage Defect Size Between Measurements Obtained on Preoperative MRI Versus During Arthrotomy (2023)
Journal Article
Perry, J., Kuiper, J. H., McCarthy, H. S., Jermin, P., Gallacher, P. D., Tins, B., & Roberts, S. (2023). Comparison of Knee Articular Cartilage Defect Size Between Measurements Obtained on Preoperative MRI Versus During Arthrotomy. Orthopaedic Journal of Sports Medicine, 11(9), Article 23259671231193380. https://doi.org/10.1177/23259671231193380

Background: Treatment decisions for cartilage defects are often based on lesion size. Magnetic resonance imaging (MRI) is widely used to diagnose cartilage defects noninvasively; however, their size estimated from MRI may differ from defect sizes me... Read More about Comparison of Knee Articular Cartilage Defect Size Between Measurements Obtained on Preoperative MRI Versus During Arthrotomy.

A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications (2023)
Journal Article
Hulme, C. H., Mennan, C., McCarthy, H. S., Davies, R., Lan, T., Rix, L., …Wright, K. (2023). A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications. Cytotherapy, 25(10), 1017-1026. https://doi.org/10.1016/j.jcyt.2023.04.004

The Quantum cell expansion system manufactured by Terumo-BCT is perhaps the most widely reported Good Manufacturing Practice–compliant bioreactor used for the expansion of adherent cell populations, both for research purposes and cl... Read More about A comprehensive review of quantum bioreactor cell manufacture: Research and clinical applications.

Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report (2023)
Journal Article
Wilkinson, H., McCarthy, H. S., Perry, J., Smith, T., Wright, K., & Cool, P. (2023). Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report. Cureus, 15(5), https://doi.org/10.7759/cureus.38788

There is currently no "gold-standard" method to diagnose prosthetic joint infections (PJI), and the current practice of using microbiological cultures has many limitations. The identification of the bacterial species causing the infection is crucial... Read More about Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report.

Histological and Radiological Assessment of Endogenously Generated Repair Tissue In Vivo Following a Chondral Harvest (2023)
Journal Article
Gallacher, P., Jermin, P., Richardson, J., Kuiper, J., Roberts, S., McCarthy, H., & Tins, B. (2023). Histological and Radiological Assessment of Endogenously Generated Repair Tissue In Vivo Following a Chondral Harvest. Cartilage, 48-58. https://doi.org/10.1177/19476035221149523

OBJECTIVE: To examine repair tissue formed approximately 15 months after a chondral harvest in the human knee. DESIGN: Sixteen individuals (12 males, 4 females, mean age 36 ± 9 years) underwent a chondral harvest in the trochlea as a pre-requisite fo... Read More about Histological and Radiological Assessment of Endogenously Generated Repair Tissue In Vivo Following a Chondral Harvest.

Osteochondral Lesions of the Ankle Treated with Bone Marrow Concentrate with Hyaluronan and Fibrin: A Single-Centre Study. (2022)
Journal Article
Abas, S., Kuiper, J. H., Roberts, S., McCarthy, H., Williams, M., Bing, A., …Makwana, N. (2022). Osteochondral Lesions of the Ankle Treated with Bone Marrow Concentrate with Hyaluronan and Fibrin: A Single-Centre Study. Cells, 11(4), https://doi.org/10.3390/cells11040629

Osteochondral defects of the ankle (OCD) are being increasingly identified as a clinically significant consequence of injury to the ankle, with the potential to lead to osteoarthritis if left untreated. The aim of this retrospective cohort study was... Read More about Osteochondral Lesions of the Ankle Treated with Bone Marrow Concentrate with Hyaluronan and Fibrin: A Single-Centre Study..

Human Mesenchymal Stromal Cells Enhance Cartilage Healing in a Murine Joint Surface Injury Model. (2021)
Journal Article
McCarthy, H., Perry, J., Mennan, C., & Wright, K. (2021). Human Mesenchymal Stromal Cells Enhance Cartilage Healing in a Murine Joint Surface Injury Model. Cells, 1-16. https://doi.org/10.3390/cells10081999

Human umbilical cord (hUC)- or bone marrow (hBM)-derived mesenchymal stromal cells (MSCs) were evaluated as an allogeneic source of cells for cartilage repair. We aimed to determine if they could enhance healing of chondral defects with or without th... Read More about Human Mesenchymal Stromal Cells Enhance Cartilage Healing in a Murine Joint Surface Injury Model..

The Management of Talar Osteochondral Lesions - Current Concepts (2021)
Journal Article
Lan, T., McCarthy, H., Hulme, C., Wright, K., & Makwana, N. (2021). The Management of Talar Osteochondral Lesions - Current Concepts. Journal of Arthroscopy and Joint Surgery, 231-237. https://doi.org/10.1016/j.jajs.2021.04.002

Osteochondral lesions of the talus (OLTs) are a common complication following trauma, involving both the articular cartilage and the underlying subchondral bone, with variable aetiologies and often presenting with non-specific symptoms. Diagnosis of... Read More about The Management of Talar Osteochondral Lesions - Current Concepts.

Perlecan in the Natural and Cell Therapy Repair of Human Adult Articular Cartilage: Can Modifications in This Proteoglycan Be a Novel Therapeutic Approach? (2021)
Journal Article
Garcia, J., McCarthy, H. S., Kuiper, J. H., Melrose, J., & Roberts, S. (2021). Perlecan in the Natural and Cell Therapy Repair of Human Adult Articular Cartilage: Can Modifications in This Proteoglycan Be a Novel Therapeutic Approach?. Biomolecules, 11(1), Article ARTN 92. https://doi.org/10.3390/biom11010092

Articular cartilage is considered to have limited regenerative capacity, which has led to the search for therapies to limit or halt the progression of its destruction. Perlecan, a multifunctional heparan sulphate (HS) proteoglycan, promotes embryonic... Read More about Perlecan in the Natural and Cell Therapy Repair of Human Adult Articular Cartilage: Can Modifications in This Proteoglycan Be a Novel Therapeutic Approach?.

Injected human umbilical cord-derived mesenchymal stromal cells do not appear to elicit an inflammatory response in a murine model of osteoarthritis (2020)
Journal Article
Perry, J., McCarthy, H., Bou-Gharios, G., van't Hof, R., Milner, P., Mennan, C., & Roberts, S. (2020). Injected human umbilical cord-derived mesenchymal stromal cells do not appear to elicit an inflammatory response in a murine model of osteoarthritis. Osteoarthritis and Cartilage Open, 2(2), 100044 - 100044. https://doi.org/10.1016/j.ocarto.2020.100044

Objective This study investigated the effect of hUC-MSCs on osteoarthritis (OA) progression in a xenogeneic model. Design Male, 10 week-old C57Bl/6 mice underwent sham surgery (n=15) or partial medial meniscectomy (PMM;n=76). 5x105 hUC-MSCs (from 3 d... Read More about Injected human umbilical cord-derived mesenchymal stromal cells do not appear to elicit an inflammatory response in a murine model of osteoarthritis.

Human Articular Chondrocytes Retain Their Phenotype in Sustained Hypoxia While Normoxia Promotes Their Immunomodulatory Potential (2019)
Journal Article
Mennan, C., Garcia, J., McCarthy, H., Owen, S., Perry, J., Wright, K., …Roberts, S. (2019). Human Articular Chondrocytes Retain Their Phenotype in Sustained Hypoxia While Normoxia Promotes Their Immunomodulatory Potential. Cartilage, 10(4), 467-479. https://doi.org/10.1177/1947603518769714

Objective To assess the phenotype of human articular chondrocytes cultured in normoxia (21% O2) or continuous hypoxia (2% O2). Design Chondrocytes were extracted from patients undergoing total knee replacement (n = 5) and cultured in ~21% (normo... Read More about Human Articular Chondrocytes Retain Their Phenotype in Sustained Hypoxia While Normoxia Promotes Their Immunomodulatory Potential.

Combined Autologous Chondrocyte and Bone Marrow Mesenchymal Stromal Cell Implantation in the Knee: An 8-year Follow Up of Two First-In-Man Cases (2019)
Journal Article
Wang, J., Wright, K., Perry, J., Tins, B., Hopkins, T., Hulme, C., …Richardson, J. (2019). Combined Autologous Chondrocyte and Bone Marrow Mesenchymal Stromal Cell Implantation in the Knee: An 8-year Follow Up of Two First-In-Man Cases. Cell Transplantation, 28(7), 924 - 931. https://doi.org/10.1177/0963689719845328

Autologous chondrocyte implantation (ACI) has been used to treat cartilage defects for >20 years, with promising clinical outcomes. Here, we report two first-in-man cases (patient A and B) treated with combined autologous chondrocyte and bone marrow... Read More about Combined Autologous Chondrocyte and Bone Marrow Mesenchymal Stromal Cell Implantation in the Knee: An 8-year Follow Up of Two First-In-Man Cases.

Human umbilical cord mesenchymal stromal cells do not evoke an immune response in the partial medial meniscectomy murine model of osteoarthritis (2019)
Journal Article
Perry, J., Mennan, C., McCarthy, H., Bou-Gharios, G., Hof, R. V. '., Milner, P., …Roberts, S. (2019). Human umbilical cord mesenchymal stromal cells do not evoke an immune response in the partial medial meniscectomy murine model of osteoarthritis. Osteoarthritis and Cartilage, 27(S1), S438-S439. https://doi.org/10.1016/j.joca.2019.02.468

Magnetic Resonance Imaging Parameters at 1 Year Correlate With Clinical Outcomes Up to 17 Years After Autologous Chondrocyte Implantation (2018)
Journal Article
McCarthy, H. S., McCall, I. W., Williams, J. M., Mennan, C., Dugard, M. N., Richardson, J. B., & Roberts, S. (2018). Magnetic Resonance Imaging Parameters at 1 Year Correlate With Clinical Outcomes Up to 17 Years After Autologous Chondrocyte Implantation. Orthopaedic Journal of Sports Medicine, https://doi.org/10.1177/2325967118788280

Background: The ability to predict the long-term success of surgical treatment in orthopaedics is invaluable, particularly in clinical trials. The quality of repair tissue formed 1 year after autologous chondrocyte implantation (ACI) in the knee was... Read More about Magnetic Resonance Imaging Parameters at 1 Year Correlate With Clinical Outcomes Up to 17 Years After Autologous Chondrocyte Implantation.

Efficacy and safety of autologous cell therapies for knee cartilage defects (autologous stem cells, chondrocytes or the two): randomized controlled trial design. (2017)
Journal Article
Richardson, J. B., Wright, K. T., Wales, J., Herman Kuiper, J., McCarthy, H. S., Gallacher, P., …Roberts, S. (2017). Efficacy and safety of autologous cell therapies for knee cartilage defects (autologous stem cells, chondrocytes or the two): randomized controlled trial design. Regenerative Medicine, 12(5), 493-501. https://doi.org/10.2217/rme-2017-0032

AIM: The main aim of this trial is to test the safety and efficacy of autologous stromal/stem cells, chondrocytes or the two combined in the treatment of knee cartilage defects. PATIENTS & METHODS: Patients with symptomatic chondral/osteochondral def... Read More about Efficacy and safety of autologous cell therapies for knee cartilage defects (autologous stem cells, chondrocytes or the two): randomized controlled trial design..

Analysis of cartilage oligomeric matrix protein in synovial fluid, plasma and repair tissue of patients treated with autologous chondrocyte implantation (2016)
Journal Article
McCarthy, H., Wright, K., Williams, J., Gallacher, P., Richardson, J., & Roberts, S. (2016). Analysis of cartilage oligomeric matrix protein in synovial fluid, plasma and repair tissue of patients treated with autologous chondrocyte implantation. International Journal of Experimental Pathology, A11 -A12. https://doi.org/10.1111/iep.12211

Meeting abstract.

Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat. (2016)
Journal Article
Garcia, J., Mennan, C., McCarthy, H. S., Roberts, S., Richardson, J. B., & Wright, K. T. (2016). Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat. Stem Cells International, 2016, Article 6969726. https://doi.org/10.1155/2016/6969726

Autologous chondrocyte implantation (ACI) is a cell-based therapy that has been used clinically for over 20 years to treat cartilage injuries more efficiently in order to negate or delay the need for joint replacement surgery. In this time, very litt... Read More about Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat..

Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton’s jelly and bone marrow (2016)
Journal Article
Mennan, C., Brown, S., McCarthy, H., Mavrogonatou, E., Kletsas, D., Garcia, J., …Roberts, S. (2016). Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton’s jelly and bone marrow. FEBS Open Bio, 6(11), 1054-1066. https://doi.org/10.1002/2211-5463.12104

Mesenchymal stromal cells (MSC) can be isolated from several regions of human umbilical cords, including Wharton's jelly (WJ), artery, vein or cord lining. These MSC appear to be immune privileged and are promising candidates for cell therapy. Howeve... Read More about Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton’s jelly and bone marrow.