Helen McCarthy h.s.mccarthy@keele.ac.uk
Histological and Radiological Assessment of Endogenously Generated Repair Tissue In Vivo Following a Chondral Harvest
McCarthy, Helen S.; Tins, Bernhard; Gallacher, Peter D.; Jermin, Paul; Richardson, James B.; Herman Kuiper, Jan; Roberts, Sally
Authors
Bernhard Tins
Peter D. Gallacher
Paul Jermin
James B. Richardson
Jan Kuiper j.h.kuiper@keele.ac.uk
Sally Roberts
Abstract
OBJECTIVE: To examine repair tissue formed approximately 15 months after a chondral harvest in the human knee. DESIGN: Sixteen individuals (12 males, 4 females, mean age 36 ± 9 years) underwent a chondral harvest in the trochlea as a pre-requisite for autologous chondrocyte implantation (ACI) treatment. The harvest site was assessed via MRI at 14.3 ± 3.2 months and arthroscopy at 15 ± 3.5 months (using the Oswestry Arthroscopy Score [O-AS] and the International Cartilage Repair Society Arthroscopy Score [ICRS-AS]). Core biopsies (1.8 mm diameter, n = 16) of repair tissue obtained at arthroscopy were assessed histologically (using the ICRS II and OsScore histology scores) and examined via immunohistochemistry for the presence of collagen types I and II. RESULTS: The mean O-AS and ICRS-AS of the repaired harvest sites were 7.2 ± 3.2 and 10.1 ± 3.5, respectively, with 80.3% ± 26% repair fill depth on MRI. The histological quality of the repair tissue formed was variable, with some hyaline cartilage present in 50% of the biopsies; where this occurred, it was associated with a significantly higher ICRS-AS than those with no hyaline cartilage present (median 11 vs. 7.5, P = 0.049). Collagen types I and II were detected in 12/14 and 10/13 biopsies, respectively. CONCLUSIONS: We demonstrate good-quality structural repair tissue formed following cartilage harvest in ACI, suggesting this site can be useful to study endogenous cartilage repair in humans. The trochlea is less commonly affected by osteoarthritis; therefore, location may be critical for spontaneous repair. Understanding the mechanisms and factors influencing this could improve future treatments for cartilage defects.
Citation
McCarthy, H. S., Tins, B., Gallacher, P. D., Jermin, P., Richardson, J. B., Herman Kuiper, J., & Roberts, S. (2023). Histological and Radiological Assessment of Endogenously Generated Repair Tissue In Vivo Following a Chondral Harvest. Cartilage, 48-58. https://doi.org/10.1177/19476035221149523
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 17, 2022 |
Publication Date | Jan 26, 2023 |
Publicly Available Date | May 30, 2023 |
Journal | CARTILAGE |
Print ISSN | 1947-6035 |
Publisher | SAGE Publications |
Pages | 48-58 |
DOI | https://doi.org/10.1177/19476035221149523 |
Keywords | articular cartilage; endogenous healing; trochlea; cartilage regeneration; osteoarthritis |
Public URL | https://keele-repository.worktribe.com/output/425292 |
Publisher URL | https://journals.sagepub.com/doi/10.1177/19476035221149523 |
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https://creativecommons.org/licenses/by/4.0/
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