All Outputs (19)

Label-Free In Situ Chemical Characterization of Amyloid Plaques in Human Brain Tissues (2024)
Journal Article

The accumulation of amyloid plaques and increased brain redox burdens are neuropathological hallmarks of Alzheimer’s disease. Altered metabolism of essential biometals is another feature of Alzheimer’s, with amyloid plaques representing sites of dist... Read More about Label-Free In Situ Chemical Characterization of Amyloid Plaques in Human Brain Tissues.

Optical Microscopy Using the Faraday Effect Reveals in Situ Magnetization Dynamics of Magnetic Nanoparticles in Biological Samples (2024)
Journal Article

The study of exogenous and endogenous nanoscale magnetic material in biology is important for developing biomedical nanotechnology as well as for understanding fundamental biological processes such as iron metabolism and biomineralization. Here, we e... Read More about Optical Microscopy Using the Faraday Effect Reveals in Situ Magnetization Dynamics of Magnetic Nanoparticles in Biological Samples.

Illuminating the brain: Revealing brain biochemistry with synchrotron X-ray spectromicroscopy (2023)
Journal Article

The synchrotron x-ray spectromicroscopy technique Scanning Transmission X-ray Microscopy (STXM) offers a powerful means to examine the underlying biochemistry of biological systems, owing to its combined chemical sensitivity and nanoscale spatial res... Read More about Illuminating the brain: Revealing brain biochemistry with synchrotron X-ray spectromicroscopy.

Biogenic metallic elements in the human brain? (2021)
Journal Article

The chemistry of copper and iron plays a critical role in normal brain function. A variety of enzymes and proteins containing positively charged Cu+, Cu2+, Fe2+, and Fe3+ control key processes, catalyzing oxidative metabolism and neurotransmitter and... Read More about Biogenic metallic elements in the human brain?.

Nanoscale chemical speciation of ß-amyloid/iron aggregates using soft x-ray spectromicroscopy (2021)
Journal Article

Iron (Fe) is an essential trace element required for healthy brain function. Yet, disrupted iron neurochemistry, and the associated formation of aberrantly aggregated protein lesions has been implicated in the development of multiple degenerative bra... Read More about Nanoscale chemical speciation of ß-amyloid/iron aggregates using soft x-ray spectromicroscopy.

Iron stored in ferritin is chemically reduced in the presence of aggregating Aß(1-42). (2020)
Journal Article

Atypical low-oxidation-state iron phases in Alzheimer's disease (AD) pathology are implicated in disease pathogenesis, as they may promote elevated redox activity and convey toxicity. However, the origin of low-oxidation-state iron and the pathways r... Read More about Iron stored in ferritin is chemically reduced in the presence of aggregating Aß(1-42)..

Analysis of neuronal iron deposits in Parkinson's disease brain tissue by synchrotron x-ray spectromicroscopy. (2020)
Journal Article

BACKGROUND: Neuromelanin-pigmented neurons, which are highly susceptible to neurodegeneration in the Parkinson's disease substantia nigra, harbour elevated iron levels in the diseased state. Whilst it is widely believed that neuronal iron is stored i... Read More about Analysis of neuronal iron deposits in Parkinson's disease brain tissue by synchrotron x-ray spectromicroscopy..

Label-Free Nanoimaging of Neuromelanin in the Brain by Soft X-ray Spectromicroscopy. (2020)
Journal Article

A hallmark of Parkinson's disease is the death of neuromelanin-pigmented neurons, but the role of neuromelanin is unclear. The in situ characterization of neuromelanin remains dependent on detectable pigmentation, rather than direct quantification of... Read More about Label-Free Nanoimaging of Neuromelanin in the Brain by Soft X-ray Spectromicroscopy..

Emerging Approaches to Investigate the Influence of Transition Metals in the Proteinopathies (2019)
Journal Article

Transition metals have essential roles in brain structure and function, and are associated with pathological processes in neurodegenerative disorders classed as proteinopathies. Synchrotron X-ray techniques, coupled with ultrahigh-resolution mass spe... Read More about Emerging Approaches to Investigate the Influence of Transition Metals in the Proteinopathies.

Metal Ion Binding to the Amyloid ß Monomer Studied by Native Top-Down FTICR Mass Spectrometry. (2019)
Journal Article

Native top-down mass spectrometry is a fast, robust biophysical technique that can provide molecular-scale information on the interaction between proteins or peptides and ligands, including metal cations. Here we have analyzed complexes of the full-l... Read More about Metal Ion Binding to the Amyloid ß Monomer Studied by Native Top-Down FTICR Mass Spectrometry..

Correlative Spectromicroscopy and Tomography for Biomedical Applications involving Electron, Ion, and Soft X-ray Microscopies (2019)
Journal Article

Many important scientific and technical problems are best addressed using multiple, microscopy-based analytical techniques that combine the strengths of complementary methods. Here, we provide two examples from biomedical challenges: unravelling the... Read More about Correlative Spectromicroscopy and Tomography for Biomedical Applications involving Electron, Ion, and Soft X-ray Microscopies.

Nanoscale Examination of Biological Tissues Using X-ray Spectromicroscopy (2018)
Journal Article

Alternating current (AC) susceptibility as a particle-focused probe of coating and clustering behaviour in magnetic nanoparticle suspensions. (2018)
Journal Article

HYPOTHESIS: The functionality of magnetic nanoparticles (MNPs) relies heavily on their surface coating, which in turn affects the interactions between MNPs, and the formation of single-core particles or multi-core clusters. In this study we assessed... Read More about Alternating current (AC) susceptibility as a particle-focused probe of coating and clustering behaviour in magnetic nanoparticle suspensions..

Nanoscale synchrotron X-ray speciation of iron and calcium compounds in amyloid plaque cores from Alzheimer’s disease subjects (2018)
Journal Article

Altered metabolism of biometals in the brain is a key feature of Alzheimer's disease, and biometal interactions with amyloid-ß are linked to amyloid plaque formation. Iron-rich aggregates, including evidence for the mixed-valence iron oxide magnetite... Read More about Nanoscale synchrotron X-ray speciation of iron and calcium compounds in amyloid plaque cores from Alzheimer’s disease subjects.

Nanoscale correlation of iron biochemistry with amyloid plaque morphology in Alzheimer’s disease transgenic mouse cortex (2017)
Journal Article

A signature characteristic of Alzheimer's disease (AD) is aggregation of amyloid-beta (Aß) fibrils in the brain. Nevertheless, the links between Aß and AD pathology remain incompletely understood. It has been proposed that neurotoxicity arising from... Read More about Nanoscale correlation of iron biochemistry with amyloid plaque morphology in Alzheimer’s disease transgenic mouse cortex.

Iron Biochemistry is Correlated with Amyloid Plaque Morphology in an Established Mouse Model of Alzheimer's Disease. (2017)
Journal Article

A signature characteristic of Alzheimer's disease (AD) is aggregation of amyloid-beta (Aß) fibrils in the brain. Nevertheless, the links between Aß and AD pathology remain incompletely understood. It has been proposed that neurotoxicity arising from... Read More about Iron Biochemistry is Correlated with Amyloid Plaque Morphology in an Established Mouse Model of Alzheimer's Disease..

ADVANCES IN X-RAY MICROSCOPY FOR THE ANALYSIS OF TRANSITION METALS IN THE BRAIN (2017)
Presentation / Conference

Introduction: X-rays have the advantage that they have a short wavelength and can penetrate through a thick biological sample. It was the need to ‘see inside’ opaque objects, especially biological tissues, and to resolve features too small for optica... Read More about ADVANCES IN X-RAY MICROSCOPY FOR THE ANALYSIS OF TRANSITION METALS IN THE BRAIN.

Ferrous iron formation following the co-aggregation of ferric iron and the Alzheimer's disease peptide ß-amyloid (1-42). (2014)
Journal Article

For decades, a link between increased levels of iron and areas of Alzheimer's disease (AD) pathology has been recognized, including AD lesions comprised of the peptide ß-amyloid (Aß). Despite many observations of this association, the relationship be... Read More about Ferrous iron formation following the co-aggregation of ferric iron and the Alzheimer's disease peptide ß-amyloid (1-42)..

Evidence of redox-active iron formation following aggregation of ferrihydrite and the Alzheimer's disease peptide ß-amyloid. (2014)
Journal Article

Recent work has demonstrated increased levels of redox-active iron biominerals in Alzheimer's disease (AD) tissue. However, the origin, nature, and role of iron in AD pathology remains unclear. Using X-ray absorption, X-ray microspectroscopy, and ele... Read More about Evidence of redox-active iron formation following aggregation of ferrihydrite and the Alzheimer's disease peptide ß-amyloid..