Outputs (44)
Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages (2024)
Journal Article
CRISPR-based gene drives have the potential to spread within populations and are considered as promising vector control tools. A doublesex-targeting gene drive was able to suppress laboratory Anopheles mosquito populations in small and large cages, a... Read More about Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages.
Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics (2024)
Journal Article
The objectives of this study were to compare dengue virus (DENV) cases, deaths, case-fatality ratio [CFR], and meteorological parameters between the first and the recent decades of this century (2000–2010 vs. 2011–2022) and to describe the trends, se... Read More about Two decades of endemic dengue in Bangladesh (2000–2022): trends, seasonality, and impact of temperature and rainfall patterns on transmission dynamics.
Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome (2023)
Journal Article
Understanding development and genetic regulation in the Anopheles gambiae germline is essential to engineer effective genetic control strategies targeting this malaria mosquito vector. These include targeting the germline to induce sterility or using... Read More about Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome.
Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome. (2023)
Journal Article
Understanding development and genetic regulation in the Anopheles gambiae germline is essential to engineer effective genetic control strategies targeting this malaria mosquito vector. These include targeting the germline to induce sterility or using... Read More about Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome..
Understanding development and genetic regulation in the Anopheles gambiae germline is essential to engineer effective genetic control strategies targeting this malaria mosquito vector. These include targeting the germline to induce sterility or using... Read More about Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome..
qSanger: Quantification of Genetic Variants in Bacterial Cultures by Sanger Sequencing (2023)
Journal Article
Genetic variations such as mutations and recombinations arise spontaneously in all cultured organisms. Although it is possible to identify nonneutral mutations by selection or counterselection, the identification of neutral mutations in a heterogeneo... Read More about qSanger: Quantification of Genetic Variants in Bacterial Cultures by Sanger Sequencing.
The Potential for a Released Autosomal X-Shredder Becoming a Driving-Y Chromosome and Invasively Suppressing Wild Populations of Malaria Mosquitoes (2021)
Journal Article
Sex-ratio distorters based on X-chromosome shredding are more efficient than sterile male releases for population suppression. X-shredding is a form of sex distortion that skews spermatogenesis of XY males towards the preferential transmission of Y-b... Read More about The Potential for a Released Autosomal X-Shredder Becoming a Driving-Y Chromosome and Invasively Suppressing Wild Populations of Malaria Mosquitoes.
Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation (2021)
Journal Article
CRISPR-based homing gene drives can be designed to disrupt essential genes whilst biasing their own inheritance, leading to suppression of mosquito populations in the laboratory. This class of gene drives relies on CRISPR-Cas9 cleavage of a target se... Read More about Resistance to a CRISPR-based gene drive at an evolutionarily conserved site is revealed by mimicking genotype fixation.
A genetically encoded anti-CRISPR protein constrains gene drive spread and prevents population suppression (2021)
Journal Article
CRISPR-based gene drives offer promising means to reduce the burden of pests and vector-borne diseases. These techniques consist of releasing genetically modified organisms carrying CRISPR-Cas nucleases designed to bias their inheritance and rapidly... Read More about A genetically encoded anti-CRISPR protein constrains gene drive spread and prevents population suppression.
Genetic Technologies for Sustainable Management of Insect Pests and Disease Vectors (2021)
Journal Article
Recent advancements in genetic and genome editing research, augmented by the discovery of new molecular tools such as CRISPR, have revolutionised the field of genetic engineering by enabling precise site-specific genome modifications with unprecedent... Read More about Genetic Technologies for Sustainable Management of Insect Pests and Disease Vectors.
A Code of Ethics for Gene Drive Research. (2021)
Journal Article
Gene drives hold promise for use in controlling insect vectors of diseases, agricultural pests, and for conservation of ecosystems against invasive species. At the same time, this technology comes with potential risks that include unknown downstream... Read More about A Code of Ethics for Gene Drive Research..
Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance (2021)
Journal Article
Homing-based gene drives use a germline source of nuclease to copy themselves at specific target sites in a genome and bias their inheritance. Such gene drives can be designed to spread and deliberately suppress populations of malaria mosquitoes by i... Read More about Regulating the expression of gene drives is key to increasing their invasive potential and the mitigation of resistance.
A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae (2020)
Journal Article
Only female insects transmit diseases such as malaria, dengue and Zika; therefore, control methods that bias the sex ratio of insect offspring have long been sought. Genetic elements such as sex-chromosome drives can distort sex ratios to produce uni... Read More about A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae.
Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics (2020)
Journal Article
CRISPR guide RNAs (gRNAs) can be programmed with relative ease to allow the genetic editing of nearly any DNA or RNA sequence. Here, we propose novel molecular architectures to achieve RNA-dependent modulation of CRISPR activity in response to specif... Read More about Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics.
Vector-Focused Approaches to Curb Malaria Transmission in the Brazilian Amazon: An Overview of Current and Future Challenges and Strategies (2020)
Journal Article
In Brazil, malaria transmission is mostly confined to the Amazon, where substantial progress has been achieved towards disease control in the past decade. Vector control has been historically considered a fundamental part of the main malaria control... Read More about Vector-Focused Approaches to Curb Malaria Transmission in the Brazilian Amazon: An Overview of Current and Future Challenges and Strategies.
Cellular mechanisms regulating synthetic sex ratio distortion in the Anopheles gambiae germline (2020)
Journal Article
Genetic control strategies aimed to bias the sex of progenies towards males present a promising new paradigm to eliminate malaria-transmitting mosquitoes. A synthetic sex-ratio distortion (SD) system was successfully engineered in Anopheles gambiae b... Read More about Cellular mechanisms regulating synthetic sex ratio distortion in the Anopheles gambiae germline.
Author Correction: A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae. (2020)
Journal Article
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
The potential for a released autosomal X-shredder becoming a driving-Y chromosome and invasively suppressing wild populations of malaria mosquitoes (2019)
Journal Article
Abstract Synthetic sex-ratio distorters based on X-chromosome shredding are predicted to be more efficient than sterile males for population suppression of malaria mosquitoes using genetic control. X-chromosome shredding operates through the targeted... Read More about The potential for a released autosomal X-shredder becoming a driving-Y chromosome and invasively suppressing wild populations of malaria mosquitoes.
High-resolution transcriptional profiling of Anopheles gambiae spermatogenesis reveals mechanisms of sex chromosome regulation (2019)
Journal Article
Although of high priority for the development of genetic tools to control malaria-transmitting mosquitoes, only a few germline-specific regulatory regions have been characterised to date and the presence of global regulatory mechanisms, such as dosag... Read More about High-resolution transcriptional profiling of Anopheles gambiae spermatogenesis reveals mechanisms of sex chromosome regulation.
Gene drives for genetic control of the malaria mosquito (2019)
Journal Article
'No abstract'
Introgression of a synthetic sex ratio distortion system from Anopheles gambiae into Anopheles arabiensis. (2019)
Journal Article
I-PpoI is a homing endonuclease that has a high cleavage activity and specificity for a conserved sequence within the ribosomal rDNA repeats, located in a single cluster on the Anopheles gambiae X chromosome. This property has been exploited to devel... Read More about Introgression of a synthetic sex ratio distortion system from Anopheles gambiae into Anopheles arabiensis..
Publisher Correction: Introgression of a synthetic sex ratio distortion system from Anopheles gambiae into Anopheles arabiensis. (2019)
Journal Article
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes. (2018)
Journal Article
Malaria is a serious global health burden, affecting more than 200 million people each year in over 90 countries, predominantly in Africa, Asia and the Americas. Since the year 2000, a concerted effort to combat malaria has reduced its incidence by m... Read More about Molecular tools and genetic markers for the generation of transgenic sexing strains in Anopheline mosquitoes..
Engineering CRISPR guide RNA riboswitches for in vivo applications (2018)
Journal Article
CRISPR-based genome editing provides a simple and scalable toolbox for a variety of therapeutic and biotechnology applications. Whilst the fundamental properties of CRISPR proved easily transferable from the native prokaryotic hosts to eukaryotic and... Read More about Engineering CRISPR guide RNA riboswitches for in vivo applications.
A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes (2018)
Journal Article
In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control differentiation of the two sexes. The female transcript, unlike the male,... Read More about A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes.
Improved CRISPR-based suppression gene drives mitigate resistance and impose a large reproductive load on laboratory-contained mosquito populations (2018)
Journal Article
Abstract CRISPR-based genes drives bias their own inheritance and can be used to modify entire populations of insect vectors of disease as a novel form of sustainable disease control. Gene drives designed to interfere with female fertility can suppre... Read More about Improved CRISPR-based suppression gene drives mitigate resistance and impose a large reproductive load on laboratory-contained mosquito populations.
Gene drives to fight malaria: current state and future directions (2018)
Journal Article
Self-propagating gene drive technologies have a number of desirable characteristics that warrant their development for the control of insect pest and vector populations, such as the malaria-transmitting mosquitoes. Theoretically easy to deploy and se... Read More about Gene drives to fight malaria: current state and future directions.
The creation and selection of mutations resistant to a gene drive over multiple generations in the malaria mosquito (2017)
Journal Article
Gene drives have enormous potential for the control of insect populations of medical and agricultural relevance. By preferentially biasing their own inheritance, gene drives can rapidly introduce genetic traits even if these confer a negative fitness... Read More about The creation and selection of mutations resistant to a gene drive over multiple generations in the malaria mosquito.
Cross-Species Y Chromosome Function Between Malaria Vectors of the Anopheles gambiae Species Complex (2017)
Journal Article
Y chromosome function, structure and evolution is poorly understood in many species, including the Anopheles genus of mosquitoes-an emerging model system for studying speciation that also represents the major vectors of malaria. While the Anopheline... Read More about Cross-Species Y Chromosome Function Between Malaria Vectors of the Anopheles gambiae Species Complex.
Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity (2017)
Journal Article
The retargeting of protein-DNA specificity, outside of extremely modular DNA binding proteins such as TAL effectors, has generally proved to be quite challenging. Here, we describe structural analyses of five different extensively retargeted variants... Read More about Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.
A CRISPR-Cas9 sex-ratio distortion system for genetic control (2016)
Journal Article
Genetic control aims to reduce the ability of insect pest populations to cause harm via the release of modified insects. One strategy is to bias the reproductive sex ratio towards males so that a population decreases in size or is eliminated altogeth... Read More about A CRISPR-Cas9 sex-ratio distortion system for genetic control.
Radical remodeling of the Y chromosome in a recent radiation of malaria mosquitoes. (2016)
Journal Article
Y chromosomes control essential male functions in many species, including sex determination and fertility. However, because of obstacles posed by repeat-rich heterochromatin, knowledge of Y chromosome sequences is limited to a handful of model organi... Read More about Radical remodeling of the Y chromosome in a recent radiation of malaria mosquitoes..
A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae (2016)
Journal Article
Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anophele... Read More about A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae.
The germline of the malaria mosquito produces abundant miRNAs, endo-siRNAs, piRNAs and 29-nt small RNAs. (2015)
Journal Article
BACKGROUND: Small RNAs include different classes essential for endogenous gene regulation and cellular defence against genomic parasites. However, a comprehensive analysis of the small RNA pathways in the germline of the mosquito Anopheles gambiae ha... Read More about The germline of the malaria mosquito produces abundant miRNAs, endo-siRNAs, piRNAs and 29-nt small RNAs..
A synthetic sex ratio distortion system for the control of the human malaria mosquito. (2014)
Journal Article
It has been theorized that inducing extreme reproductive sex ratios could be a method to suppress or eliminate pest populations. Limited knowledge about the genetic makeup and mode of action of naturally occurring sex distorters and the prevalence of... Read More about A synthetic sex ratio distortion system for the control of the human malaria mosquito..
Site-specific genetic engineering of the Anopheles gambiae Y chromosome. (2014)
Journal Article
Despite its function in sex determination and its role in driving genome evolution, the Y chromosome remains poorly understood in most species. Y chromosomes are gene-poor, repeat-rich and largely heterochromatic and therefore represent a difficult t... Read More about Site-specific genetic engineering of the Anopheles gambiae Y chromosome..
Engineering mosquito population for vector control. (2012)
Journal Article
Bactrocera dorsalis Hendel is a highly invasive horticultural pest of major economic importance worldwide. In Burkina Faso, it is one of the main insect pests constraining the mango production and export. Understanding the biology and the genetic dyn... Read More about Genetic Diversity and Population Structure of the Invasive Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae) in Burkina Faso.