Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance.

Tickle, JA; Jenkins, SI; Polyak, B; Pickard, MR; Chari, DM

Abstract

AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.

Citation

Tickle, J., Jenkins, S., Polyak, B., Pickard, M., & Chari, D. (2016). Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance. Nanomedicine, 345 - 358. https://doi.org/10.2217/nnm.15.202

• Acceptance Date: Jan 1, 2016
• Publication Date: Feb 1, 2016
• Journal: Nanomedicine
• Print ISSN: 1743-5889
• Publisher: Future Medicine
• Pages: 345 - 358
• DOI: https://doi.org/10.2217/nnm.15.202
• Keywords: Astrocytes, magnetite, magnetolabeling, cell transplantation, polymeric particles, label dilution
• Publisher URL: http://www.futuremedicine.com/doi/10.2217/nnm.15.202