Endocytotic potential governs magnetic particle loading in dividing neural cells: studying modes of particle inheritance.
Tickle, JA; Jenkins, SI; Polyak, B; Pickard, MR; Chari, DM
AIM: To achieve high and sustained magnetic particle loading in a proliferative and endocytotically active neural transplant population (astrocytes) through tailored magnetite content in polymeric iron oxide particles. MATERIALS & METHODS: MPs of varying magnetite content were applied to primary-derived rat cortical astrocytes ± static/oscillating magnetic fields to assess labeling efficiency and safety. RESULTS: Higher magnetite content particles display high but safe accumulation in astrocytes, with longer-term label retention versus lower/no magnetite content particles. Magnetic fields enhanced loading extent. Dynamic live cell imaging of dividing labeled astrocytes demonstrated that particle distribution into daughter cells is predominantly 'asymmetric'. CONCLUSION: These findings could inform protocols to achieve efficient MP loading into neural transplant cells, with significant implications for post-transplantation tracking/localization.
|Acceptance Date||Jan 1, 2016|
|Publication Date||Feb 1, 2016|
|Pages||345 - 358|
|Keywords||Astrocytes, magnetite, magnetolabeling, cell transplantation, polymeric particles, label dilution|
Tickle2015-Nanomedicine, Endocytotic Potential.docx
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