Skip to main content

Research Repository

Advanced Search

Expression and subcellular localization of cyclin D1 protein in epithelial ovarian tumour cells

Dhar, K K; Branigan, K; Parkes, J; Howells, R E J; Hand, P; Musgrove, C; Strange, R C; Fryer, A A; Redman, C W E; Hoban, P R

Expression and subcellular localization of cyclin D1 protein in epithelial ovarian tumour cells Thumbnail


Authors

K K Dhar

K Branigan

J Parkes

R E J Howells

P Hand

C Musgrove

R C Strange

C W E Redman

P R Hoban



Abstract

The expression of cyclin D1 protein in tumour sections from 81 patients with epithelial ovarian cancer was analysed using immunohistochemistry. The tumours that overexpressed cyclin D1 in more than 10% of neoplastic cells were considered positive. Thus overexpression of cyclin D1 was observed in 72/81 (89%) of the cases examined. Protein was detected in both the nucleus and the cytoplasm in 24/81 (30%) and localized exclusively in the cytoplasm in 48/81 (59%) of the tumours. Cyclin D1 was overexpressed in both borderline and invasive tumours. There was no association between protein overexpression and tumour stage and differentiation. Furthermore, no correlation between cyclin D1 expression and clinical outcome was observed. However, in tumours overexpressing cyclin D1 (n = 72), the proportion displaying exclusively cytoplasmic localization of protein was higher in those with serous compared with non-serous histology (P = 0.004, odds ratio 4.8, 95% confidence interval 1.4-19.1). Western analysis using a monoclonal antibody to cyclin D1 identified a 36 kDa protein in homogenates from seven tumours displaying cytoplasmic only and one tumour demonstrating both nuclear and cytoplasmic immunostaining. Using restriction fragment length polymorphism polymerase chain reaction and PCR-multiplex analysis, amplification of the cyclin D1 gene (CCND1 was detected in 1/29 of the tumours demonstrating overexpression of cyclin D1 protein. We conclude that deregulation of CCND1 expression leading to both cytoplasmic and nuclear protein localization is a frequent event in ovarian cancer and occurs mainly in the absence of gene amplification.

Citation

Dhar, K. K., Branigan, K., Parkes, J., Howells, R. E. J., Hand, P., Musgrove, C., …Hoban, P. R. (1999). Expression and subcellular localization of cyclin D1 protein in epithelial ovarian tumour cells. British journal of cancer, 81, 1174 -1181. https://doi.org/10.1038/sj.bjc.6690826

Journal Article Type Article
Acceptance Date May 13, 1999
Online Publication Date Nov 12, 1999
Publication Date Dec 1, 1999
Journal British Journal of Cancer
Print ISSN 1532-1827
Publisher Cancer Research UK
Peer Reviewed Peer Reviewed
Volume 81
Pages 1174 -1181
DOI https://doi.org/10.1038/sj.bjc.6690826
Keywords ovarian cancer, cyclin D1, cytoplasm, nucleus, amplification, clinical outcome, blotting, western, carcinoma, cyclin D1, female, humans, immunohistochemistry, neoplasm proteins, ovarian neoplasms, polymerase chain reaction, polymorphism, restriction fragm
Publisher URL https://doi.org/10.1038/sj.bjc.6690826
PMID 10584879

Files

Expression and subcellular localization of cyclin D1 protein in epithelial ovarian tumour cells.pdf (326 Kb)
PDF






You might also like



Downloadable Citations