Watkinson
Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase
Watkinson
Authors
Abstract
Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM.
Citation
Watkinson. (2019). Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase. Sensors, https://doi.org/10.3390/s19071677
Acceptance Date | Apr 2, 2019 |
---|---|
Publication Date | Apr 8, 2019 |
Journal | Sensors |
Publisher | MDPI |
DOI | https://doi.org/10.3390/s19071677 |
Keywords | protease biosensor; collagenase; poly(ethylene glycol) norbornene; QCM; hydrogel degradation; peptide cross-links; click chemistry |
Publisher URL | https://doi.org/10.3390/s19071677 |
Files
sensors-19-01677-v2.pdf
(2.3 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Synthetic Strategies for FRET-Enabled Carbohydrate Active Enzyme Probes
(2021)
Book Chapter
Developments in the Chemical Synthesis of Heparin and Heparan Sulfate
(2021)
Journal Article
Subcellular localised small molecule fluorescent probes to image mobile Zn2+
(2020)
Journal Article
Downloadable Citations
About Keele Repository
Administrator e-mail: research.openaccess@keele.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search