An alternative modular 'click-SNAr-click' approach to develop subcellular localised fluorescent probes to image mobile Zn2+

Watkinson, Mike

Abstract

Zn2+ is involved in a number of biological processes and its wide-ranging roles at the subcellular level, especially in specific organelles, have not yet been fully established due to a lack of tools to image it effectively. We report a new and efficient modular double ‘click’ approach towards a range of sub-cellular localised probes for mobile zinc. Through this methodology, endoplasmic reticulum, mitochondria and lysosome localised probes were successfully prepared which show good fluorescence responses to mobile Zn2+ in vitro and in cellulo whilst a non-targeting probe was synthesized as a control. The methodology appears to have wide-utility for the generation of sub-cellular localised probes by incorporating specific organelle targeting vectors for mobile Zn2+ imaging.

Citation

Watkinson, M., Fang, L., Trigiante, G., Crespo-Otero, R., Philpott, M. P., & Jones, C. R. (2019). An alternative modular 'click-SNAr-click' approach to develop subcellular localised fluorescent probes to image mobile Zn2+. Organic and Biomolecular Chemistry, 17(47), 10013-10019. https://doi.org/10.1039/c9ob01855g

• Journal Article Type: Article
• Acceptance Date: Oct 8, 2019
• Publication Date: Dec 21, 2019
• Publicly Available Date: May 26, 2023
• Journal: ORGANIC & BIOMOLECULAR CHEMISTRY
• Print ISSN: 1477-0520
• Publisher: Royal Society of Chemistry
• Volume: 17
• Issue: 47
• Pages: 10013-10019
• DOI: https://doi.org/10.1039/c9ob01855g
• Publisher URL: http://doi.org/10.1039/C9OB01855G