Yehonatan Alcalay
The Potential for a Released Autosomal X-Shredder Becoming a Driving-Y Chromosome and Invasively Suppressing Wild Populations of Malaria Mosquitoes
Alcalay, Yehonatan; Fuchs, Silke; Galizi, Roberto; Bernardini, Federica; Elaine Haghighat-Khah, Roya; Rusch, Douglas B.; Adrion, Jeffrey R.; Hahn, Matthew W.; Tortosa, Pablo; Rotenberry, Rachel; Aris Papathanos, Philippos
Authors
Silke Fuchs
Roberto Galizi r.galizi@keele.ac.uk
Federica Bernardini
Roya Elaine Haghighat-Khah
Douglas B. Rusch
Jeffrey R. Adrion
Matthew W. Hahn
Pablo Tortosa
Rachel Rotenberry
Philippos Aris Papathanos
Abstract
Sex-ratio distorters based on X-chromosome shredding are more efficient than sterile male releases for population suppression. X-shredding is a form of sex distortion that skews spermatogenesis of XY males towards the preferential transmission of Y-bearing gametes, resulting in a higher fraction of sons than daughters. Strains harboring X-shredders on autosomes were first developed in the malaria mosquito Anopheles gambiae, resulting in strong sex-ratio distortion. Since autosomal X-shredders are transmitted in a Mendelian fashion and can be selected against, their frequency in the population declines once releases are halted. However, unintended transfer of X-shredders to the Y-chromosome could produce an invasive meiotic drive element, that benefits from its biased transmission to the predominant male-biased offspring and its effective shielding from female negative selection. Indeed, linkage to the Y-chromosome of an active X-shredder instigated the development of the nuclease-based X-shredding system. Here, we analyze mechanisms whereby an autosomal X-shredder could become unintentionally Y-linked after release by evaluating the stability of an established X-shredder strain that is being considered for release, exploring its potential for remobilization in laboratory and wild-type genomes of An. gambiae and provide data regarding expression on the mosquito Y-chromosome. Our data suggest that an invasive X-shredder resulting from a post-release movement of such autosomal transgenes onto the Y-chromosome is unlikely.
Citation
Alcalay, Y., Fuchs, S., Galizi, R., Bernardini, F., Elaine Haghighat-Khah, R., Rusch, D. B., …Aris Papathanos, P. (2021). The Potential for a Released Autosomal X-Shredder Becoming a Driving-Y Chromosome and Invasively Suppressing Wild Populations of Malaria Mosquitoes. Frontiers in Bioengineering and Biotechnology, https://doi.org/10.3389/fbioe.2021.752253
Acceptance Date | Oct 15, 2021 |
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Publication Date | Dec 3, 2021 |
Journal | Frontiers in Bioengineering and Biotechnology |
Publisher | Frontiers Media |
DOI | https://doi.org/10.3389/fbioe.2021.752253 |
Keywords | gene drive; malaria; sex-ratio distortion; genetic control; risk assessment |
Public URL | https://keele-repository.worktribe.com/output/421776 |
Publisher URL | https://www.frontiersin.org/articles/10.3389/fbioe.2021.752253/full |
Files
fbioe-09-752253.pdf
(1.4 Mb)
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Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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