Defining a turnover index for the correlation of biomaterial degradation and cell based extracellular matrix synthesis using fluorescent tagging techniques

Bardsley, Katie; Wimpenny, Ian; Wechsler, Roni; Shachaf, Yonatan; Yang, Ying; El Haj, Alicia J.

Abstract

Non-destructive protocols which can define a biomaterial’s degradation and its associated ability to support proliferation and/or promote extracellular matrix deposition will be an essential in vitro tool. In this study we investigate fluorescently tagged biomaterials, with varying rates of degradation and their ability to support cell proliferation and osteogenic differentiation. Changes in fluorescence of the biomaterials and the release of fluorescent soluble by-products were confirmed as accurate methods to quantify degradation. It was demonstrated that increasing rates of the selected biomaterials’ degradation led to a decrease in cell proliferation and concurrently an increase in osteogenic matrix production. A novel turnover index (TI), which directly describes the effect of degradation of a biomaterial on cell behaviour, was calculated. Lower TIs for proliferation and high TIs for osteogenic marker production were observed on faster degrading biomaterials, indicating that these biomaterials supported an upregulation of osteogenic markers. This TI was further validated using an ex vivo chick femur model, where the faster degrading biomaterial, fibrin, led to an increased TI for mineralisation within an epiphyseal defect. This in vitro tool, TI, for monitoring the effect of biomaterial degradation on extracellular matrix production may well act as predictor of the selected biomaterials’ performance during in vivo studies.

Citation

Bardsley, K., Wimpenny, I., Wechsler, R., Shachaf, Y., Yang, Y., & El Haj, A. J. (2016). Defining a turnover index for the correlation of biomaterial degradation and cell based extracellular matrix synthesis using fluorescent tagging techniques. Acta biomaterialia, 45, 133-142. https://doi.org/10.1016/j.actbio.2016.09.002

• Journal Article Type: Article
• Acceptance Date: Sep 1, 2016
• Online Publication Date: Sep 2, 2016
• Publication Date: 2016-11
• Deposit Date: Jun 8, 2023
• Journal: Acta Biomaterialia
• Print ISSN: 1742-7061
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 45
• Pages: 133-142
• DOI: https://doi.org/10.1016/j.actbio.2016.09.002
• Keywords: Molecular Biology; Biomedical Engineering; Biochemistry; Biomaterials; General Medicine; Biotechnology
• Additional Information: This article is maintained by: Elsevier; Article Title: Defining a turnover index for the correlation of biomaterial degradation and cell based extracellular matrix synthesis using fluorescent tagging techniques; Journal Title: Acta Biomaterialia; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.actbio.2016.09.002; Content Type: article; Copyright: © 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.