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Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model

Perry, Jade; Mennan, Claire; Cool, Paul; McCarthy, Helen; Newell, Karin; Hopkins, Tim; Hulme, Charlotte; Wright, Karina; Henson, Frances; Roberts, Sally

Authors

Karin Newell

Tim Hopkins

Frances Henson

Sally Roberts



Abstract

Objective
To determine if mesenchymal stromal cells (MSCs) derived from human umbilical cords (hUC) could reduce degeneration developing when injected into the knee of a large animal model of osteoarthritis (OA).
Design
Ten million culture-expanded UC-MSCs (pooled from 3 human donors) were injected in 50 μL of tissue culture medium into the left stifle joints of 7 sheep whose medial meniscus was transected 4 weeks previously. Seven other sheep had only 50 μL of medium injected as the no treatment “control” group. After 8 weeks the sheep underwent euthanasia, the joints were excised and examined macroscopically, via x-ray and magnetic resonance imaging (MRI), both via histology for degenerative and inflammatory changes and immunohistochemically to identify any human cells within the joint tissues. Activity monitoring both before meniscus transection and euthanasia was also undertaken.
Results
There was a significant reduction in the Kellgren–Lawrence x-ray score for joints injected with hUC-MSCs compared with the control joints. Likewise, macroscopic, MRI, synovitis and OARSI histology scores were all lower (better) in the joints injected with hUC-MSCs than in the control arm, but not significantly. Activity levels and synovitis scores were similar in both groups of animals.
Conclusions
hUC-MSCs appear to modify and reduce the development of osteoarthritic changes in the ovine stifle joint after meniscal destabilization, an injury which commonly leads to OA in humans. These results are encouraging for the potential benefit of culture expanded UC-MSCs as an allogeneic cell therapy in patients who may have early OA following a meniscal injury of the knee.

Citation

Perry, J., Mennan, C., Cool, P., McCarthy, H., Newell, K., Hopkins, T., …Roberts, S. (2024). Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model. Cartilage, https://doi.org/10.1177/19476035241287832

Journal Article Type Article
Acceptance Date Sep 14, 2024
Online Publication Date Nov 3, 2024
Publication Date Nov 3, 2024
Deposit Date Oct 8, 2024
Publicly Available Date Dec 16, 2024
Journal Cartilage
Print ISSN 1947-6035
Publisher SAGE Publications
Peer Reviewed Peer Reviewed
DOI https://doi.org/10.1177/19476035241287832
Keywords cell therapy; allogeneic; mesenchymal stem cells; cells; osteoarthritis; diagnosis; preclinical models
Public URL https://keele-repository.worktribe.com/output/949504

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Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model (106 Kb)
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Licence
https://creativecommons.org/licenses/by/4.0/

Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/

Copyright Statement
This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page






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