All Outputs (49)

19 F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy (2016)
Journal Article
Lima, M. A., Cavalheiro, R. P., Viana, G. M., Meneghetti, M. C., Rudd, T. R., Skidmore, M., …Yates, E. A. (2016). 19 F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy. Glycoconjugate Journal, 34, 405-410. https://doi.org/10.1007/s10719-016-9723-x

Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues using microscopy, to enab... Read More about 19 F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy.

Atomic Details of the Interactions of Glycosaminoglycans with Amyloid-beta Fibrils (2016)
Journal Article
Stewart, K. L., Hughes, E., Yates, E. A., Akien, G. R., Huang, T., Andrade De Lima, M., …Middleton, D. A. (2016). Atomic Details of the Interactions of Glycosaminoglycans with Amyloid-beta Fibrils. Journal of the American Chemical Society, 138(27), 8328-8331. https://doi.org/10.1021/jacs.6b02816

The amyloid plaques associated with Alzheimer’s disease (AD) comprise fibrillar amyloid-ß (Aß) peptides as well as non-protein factors including glycosaminoglycan (GAG) polysaccharides. GAGs affect the kinetics and pathway of Aß self-assembly and can... Read More about Atomic Details of the Interactions of Glycosaminoglycans with Amyloid-beta Fibrils.

Trisulfate disaccharide decreases calcium overload and protects liver injury secondary to liver ischemia/reperfusion (2016)
Journal Article
Vasques, E., Cunha, J., Coelho, A., Sampietre, S., Patzina, R., Abdo, E., …D’Albuquerque, L. A. (2016). Trisulfate disaccharide decreases calcium overload and protects liver injury secondary to liver ischemia/reperfusion. PloS one, 11, e0149630--e0149630. https://doi.org/10.1371/journal.pone.0149630

Background
Ischemia and reperfusion (I/R) causes tissue damage and intracellular calcium levels are a factor of cell death. Sodium calcium exchanger (NCX) regulates calcium extrusion and Trisulfated Disaccharide (TD) acts on NCX decreasing intracell... Read More about Trisulfate disaccharide decreases calcium overload and protects liver injury secondary to liver ischemia/reperfusion.

P2X 7 receptor activity regulation: the role of CD44 proteoglycan GAG chains (2015)
Journal Article
Moura, G., Lucena, S., Andrade De Lima, M., Nascimento, F., Gesteira, T., Nader, H., …Tersariol, I. (2015). P2X 7 receptor activity regulation: the role of CD44 proteoglycan GAG chains. Cell death and disease, https://doi.org/10.1038/cddis.2015.340

P2X7 receptors have received special attention in the literature for their involvement in several diseases characterized by inflammatory processes such as cancer, arthritis, neurodegenerative pathologies and chronic pains.

Post-translational allosteric activation of the P2X 7 receptor through glycosaminoglycan chains of CD44 proteoglycans (2015)
Journal Article
Moura, G., Lucena, S., Andrade De Lima, M., Nascimento, F., Gesteira, T., Nader, H., …Tersariol, I. (2015). Post-translational allosteric activation of the P2X 7 receptor through glycosaminoglycan chains of CD44 proteoglycans. Cell Death Discovery, 1, 15005--15005. https://doi.org/10.1038/cddiscovery.2015.5

Here, we present evidence for the positive allosteric modulation of the P2X7 receptor through glycosaminoglycans (GAGs) in CHO (cell line derived from the ovary of the Chinese hamster) cells. The marked potentiation of P2X7 activity through GAGs in t... Read More about Post-translational allosteric activation of the P2X 7 receptor through glycosaminoglycan chains of CD44 proteoglycans.

Quinolone resistance and ornithine decarboxylation activity in lactose-negative Escherichia coli (2015)
Journal Article
Gomig, F., Galvão, C. W., Freitas, D. L. D., Labas, L., Etto, R. M., Esmerino, L. A., …Silveira, R. B. D. (2015). Quinolone resistance and ornithine decarboxylation activity in lactose-negative Escherichia coli. Brazilian Journal of Microbiology, 46(3), 753-757. https://doi.org/10.1590/S1517-838246320131291

Quinolones and fluoroquinolones are widely used to treat uropathogenic Escherichia coli infections. Bacterial resistance to these antimicrobials primarily involves mutations in gyrA and parC genes. To date, no studies have examined the potential rela... Read More about Quinolone resistance and ornithine decarboxylation activity in lactose-negative Escherichia coli.

SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells (2015)
Journal Article
Vicente, C., Andrade De Lima, M., Nader, H., & Toma, L. (2015). SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells. Journal of Experimental & Clinical Cancer Research, 34, 25--25. https://doi.org/10.1186/s13046-015-0141-x

Background
SULF2 is a 6-O-endosulfatase which removes 6-O sulfate residues from N-glucosamine present on heparan sulfate (HS). The sulfation pattern of HS influences signaling events mediated by heparan sulfate proteoglycans (HSPGs) located on cell... Read More about SULF2 overexpression positively regulates tumorigenicity of human prostate cancer cells.

On the catalytic mechanism of polysaccharide lyases: evidence of His and Tyr involvement in heparin lysis by heparinase I and the role of Ca2+ (2013)
Journal Article
Córdula, C. R., Lima, M. A., Shinjo, S. K., Gesteira, T. F., Pol-Fachin, L., Coulson-Thomas, V. J., …Tersariol, I. L. S. (2013). On the catalytic mechanism of polysaccharide lyases: evidence of His and Tyr involvement in heparin lysis by heparinase I and the role of Ca2+. Molecular BioSystems, 10(1), 54-64. https://doi.org/10.1039/C3MB70370C

The structurally diverse polysaccharide lyase enzymes are distributed from plants to animals but share common catalytic mechanisms. One, heparinase I (F. heparinum), is employed in the production of the major anticoagulant drug, low molecular weight... Read More about On the catalytic mechanism of polysaccharide lyases: evidence of His and Tyr involvement in heparin lysis by heparinase I and the role of Ca2+.

Heparin inhibits cellular invasion by SARS-CoV-2: structural dependence of the interaction of the surface protein (spike) S1 receptor binding domain with heparin
Other
Mycroft-West, C. J., Su, D., Pagani, I., Rudd, T. R., Elli, S., Guimond, S. E., …Skidmore, M. A. Heparin inhibits cellular invasion by SARS-CoV-2: structural dependence of the interaction of the surface protein (spike) S1 receptor binding domain with heparin. [Preprint article]