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Professor Wim Cool's Outputs (67)

Bone amyloidosis: A rare case in a young adult (2025)
Journal Article
Inês Magalhães Rodrigues, M., Kaur, S., Cool, P., Tyrrell, P., & Winn, N. (2025). Bone amyloidosis: A rare case in a young adult. EURORAD, https://doi.org/10.35100/EURORAD/CASE.18903

A 26-year-old male presented with progressively worsening pain in his right leg, along with increasing thigh swelling
and leg shortening. Four years prior, he had sustained a right femur fracture, which was managed conservatively.
Initial investi... Read More about Bone amyloidosis: A rare case in a young adult.

A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome (2025)
Journal Article
Harrington, G. M. B., Cool, P., Hulme, C., Stokes-Fisher, J., Peffers, M., El Masri, W., Osman, A., Chowdhury, J. R., Kumar, N., Budithi, S., & Wright, K. (2025). A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome. Journal of Neurotrauma, 42(3-4), https://doi.org/10.1101/2022.07.12.499696

Spinal cord injury (SCI) is a major cause of disability, with complications postinjury often leading to lifelong health issues with the need for extensive treatment. Neurological outcome post-SCI can be variable and difficult to predict, particularly... Read More about A comprehensive proteomic and bioinformatics analysis of human spinal cord injury plasma identifies proteins associated with the complement cascade and liver function as potential prognostic indicators of neurological outcome.

A Comprehensive Proteomic and Bioinformatic Analysis of Human Spinal Cord Injury Plasma Identifies Proteins Associated with the Complement Cascade and Liver Function as Potential Prognostic Indicators of Neurological Outcome (2025)
Journal Article
Mateus Bernardo Harrington, G., Cool, P., Hulme, C., Fisher-Stokes, J., Peffers, M., El Masri, W., Osman, A., Roy Chowdhury, J., Kumar, N., Budithi, S., & Wright, K. (2025). A Comprehensive Proteomic and Bioinformatic Analysis of Human Spinal Cord Injury Plasma Identifies Proteins Associated with the Complement Cascade and Liver Function as Potential Prognostic Indicators of Neurological Outcome. Journal of Neurotrauma, 42(3-4), 292-306. https://doi.org/10.1089/neu.2023.0064

Spinal cord injury (SCI) is a major cause of disability, with complications postinjury often leading to lifelong health issues with the need for extensive treatment. Neurological outcome post-SCI can be variable and difficult to predict, particularly... Read More about A Comprehensive Proteomic and Bioinformatic Analysis of Human Spinal Cord Injury Plasma Identifies Proteins Associated with the Complement Cascade and Liver Function as Potential Prognostic Indicators of Neurological Outcome.

A deep learning framework deploying segment anything to detect pan-cancer mitotic figures from haematoxylin and eosin-stained slides (2024)
Journal Article
Shen, Z., Simard, M., Brand, D., Andrei, V., Al-Khader, A., Oumlil, F., Trevers, K., Butters, T., Haefliger, S., Kara, E., Amary, F., Tirabosco, R., Cool, P., Royle, G., Hawkins, M. A., Flanagan, A. M., & Collins-Fekete, C.-A. (in press). A deep learning framework deploying segment anything to detect pan-cancer mitotic figures from haematoxylin and eosin-stained slides. Communications Biology, 7(1), 1674. https://doi.org/10.1038/s42003-024-07398-6

Mitotic activity is an important feature for grading several cancer types. However, counting mitotic figures (cells in division) is a time-consuming and laborious task prone to inter-observer variation. Inaccurate recognition of MFs can lead to incor... Read More about A deep learning framework deploying segment anything to detect pan-cancer mitotic figures from haematoxylin and eosin-stained slides.

Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model (2024)
Journal Article
Perry, J., Mennan, C., Cool, P., McCarthy, H. S., Newell, K., Hopkins, T., Hulme, C., Wright, K. T., Henson, F. M., & Roberts, S. (2024). Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model. Cartilage, https://doi.org/10.1177/19476035241287832

Objective To determine if mesenchymal stromal cells (MSCs) derived from human umbilical cords (hUC) could reduce degeneration developing when injected into the knee of a large animal model of osteoarthritis (OA). Design Ten million culture-expanded U... Read More about Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model.

Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model (2024)
Journal Article
Perry, J., Mennan, C., Cool, P., McCarthy, H., Newell, K., Hopkins, T., Hulme, C., Wright, K., Henson, F., & Roberts, S. (2024). Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model. Cartilage, https://doi.org/10.1177/19476035241287832

Objective
To determine if mesenchymal stromal cells (MSCs) derived from human umbilical cords (hUC) could reduce degeneration developing when injected into the knee of a large animal model of osteoarthritis (OA).
Design
Ten million culture-expande... Read More about Intra-Articular Injection of Human Umbilical Cord-Derived Mesenchymal Stromal Cells Reduces Radiographic Osteoarthritis in an Ovine Model.

681 USING NANOPORE SEQUENCING TO DIAGNOSE PROSTHETIC JOINT INFECTIONS (2024)
Presentation / Conference
Wilkinson, H., Cool, P., Perry, J., Wright, K. T., McCarthy, H. S., & Hulme, C. H. (2024, April). 681 USING NANOPORE SEQUENCING TO DIAGNOSE PROSTHETIC JOINT INFECTIONS. Poster presented at 2024 OARSI World Congress on Osteoarthritis, Vienna, Austria

Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study (2024)
Journal Article
Wilkinson, H., McDonald, J., McCarthy, H., Perry, J., Wright, K., Hulme, C., & Cool, P. (in press). Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study. Briefings in Functional Genomics, Article elae008. https://doi.org/10.1093/bfgp/elae008

This project investigates if third-generation genomic sequencing can be used to identify the species of bacteria causing prosthetic joint infections (PJIs) at the time of revision surgery. Samples of prosthetic fluid were taken during revision surger... Read More about Using Nanopore sequencing to identify bacterial infection in joint replacements: A Preliminary study.

PRELIMINARY FINDINGS ON THE USE OF GENOMIC SEQUENCING TO DIAGNOSE ORTHOPAEDIC INFECTIONS (2023)
Presentation / Conference Contribution
Wilkinson, H., & Cool, P. PRELIMINARY FINDINGS ON THE USE OF GENOMIC SEQUENCING TO DIAGNOSE ORTHOPAEDIC INFECTIONS. Presented at The British Orthopaedic Research Society (BORS) 2023 Meeting (25–26 Sept 2023), Cambridge, England, UK

AbstractObjectivesThe objective of this study is to investigate if genomic sequencing is a useful method to diagnose orthopaedic infections. Current methods used to identify the species of bacteria causing orthopaedic infections take considerable tim... Read More about PRELIMINARY FINDINGS ON THE USE OF GENOMIC SEQUENCING TO DIAGNOSE ORTHOPAEDIC INFECTIONS.

Aneurysmal bone cysts: A UK wide tumor center experience (2023)
Journal Article
Bavan, L., Eastley, N., Stevenson, J., Mifsud, M., Bayliss, L., Mahmoud, S., Baker, G., Cusick, L., Nail, R., Rankin, K., Crooks, S., Cool, P., Williams, D., Kandarakis, G., Duncan, R., & Kothari, A. (in press). Aneurysmal bone cysts: A UK wide tumor center experience. Journal of Surgical Oncology, 129(3), 601-608. https://doi.org/10.1002/jso.27499

Background and Objectives
This multicenter retrospective series of consecutive extra-spinal aneurysmal bone cysts aims to identify risk factors for treatment failure.

Methods
Aneurysmal bone cysts treated within seven collaborating centers with... Read More about Aneurysmal bone cysts: A UK wide tumor center experience.

Machine learning models based on routinely sampled blood tests can predict the presence of malignancy amongst patients with suspected musculoskeletal malignancy (2023)
Journal Article
Bentick, K., Runevic, J., Akula, S., Kyriacou, T., Cool, P., & Andras, P. (2023). Machine learning models based on routinely sampled blood tests can predict the presence of malignancy amongst patients with suspected musculoskeletal malignancy. Methods, 220, 55-60. https://doi.org/10.1016/j.ymeth.2023.10.012

This study explores the possibility of using routinely taken blood tests in the diagnosis and triage of patients with suspected musculoskeletal malignancy. A retrospective study was performed on results of patients who had presented for assessment to... Read More about Machine learning models based on routinely sampled blood tests can predict the presence of malignancy amongst patients with suspected musculoskeletal malignancy.

The British Orthopaedic Oncology Management (BOOM) audit (2023)
Journal Article
Archer, J. E., Chauhan, G. S., Dewan, V., Osman, K., Thomson, C., Nandra, R. S., Ashford, R. U., Cool, P., & Stevenson, J. (2023). The British Orthopaedic Oncology Management (BOOM) audit. The Bone & Joint Journal, 105-B(10), 1115-1122. https://doi.org/10.1302/0301-620x.105b10.bjj-2023-0443.r1

AimsMost patients with advanced malignancy suffer bone metastases, which pose a significant challenge to orthopaedic services and burden to the health economy. This study aimed to assess adherence to the British Orthopaedic Oncology Society (BOOS)/Br... Read More about The British Orthopaedic Oncology Management (BOOM) audit.

Clinical outcome of open ankle fractures in patients above 70 years of age (2023)
Journal Article
Zahra, W., Seifo, M., Cool, P., Ford, D., & Okoro, T. (2023). Clinical outcome of open ankle fractures in patients above 70 years of age. World Journal of Orthopedics, 14(7), 554-561. https://doi.org/10.5312/wjo.v14.i7.554

Background: Open fractures of the ankle are complex injuries requiring multidisciplinary input and are associated with significant morbidity and mortality. However, data on the clinical outcomes of open ankle fracture management in patients older tha... Read More about Clinical outcome of open ankle fractures in patients above 70 years of age.

Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report (2023)
Journal Article
Wilkinson, H., McCarthy, H. S., Perry, J., Smith, T., Wright, K., & Cool, P. (2023). Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report. Cureus, 15(5), https://doi.org/10.7759/cureus.38788

There is currently no "gold-standard" method to diagnose prosthetic joint infections (PJI), and the current practice of using microbiological cultures has many limitations. The identification of the bacterial species causing the infection is crucial... Read More about Genomic Sequencing to Diagnose Prosthetic Joint Infection in the Knee: A Case Report.

Umbilical Cord MSC Treated Osteoarthritic Sheep Display Reduced Radiographic Changes: Proteomic Biomarker Profiling of Synovial Fluids Implicate an Immunomodulatory Mechanism (2023)
Presentation / Conference Contribution
Perry, J., Mennan, C., Cool, P., Mccarthy, H., Newell, K., Hulme, C., Wright, K., Mcdonald, J., Peffers, M., Henson, F., & Roberts, S. (2023, March). Umbilical Cord MSC Treated Osteoarthritic Sheep Display Reduced Radiographic Changes: Proteomic Biomarker Profiling of Synovial Fluids Implicate an Immunomodulatory Mechanism. Presented at TERMIS 2023 – European Chapter, Manchester Central Conference Centre Manchester, UK

pTERT mutation in the risk-stratification of solitary fibrous tumours (2023)
Presentation / Conference Contribution
Butters, T., Andrei, V., Hepburn, E., Connolly, C., Mendes, B., Haefliger, S., Machado, I., D'Arcy, C., Brandner, S., Cool, P., & Flanagan, A. (2023, January). pTERT mutation in the risk-stratification of solitary fibrous tumours. Presented at WINTER MEETING, 5th Joint Meeting with the Royal Society of Medicine, 214th Scientific Meeting of the Pathological Society of Great Britain & Ireland, Royal Society of Medicine, London

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Pages: S1-S4

A genetic model for central chondrosarcoma evolution correlates with patient outcome (2022)
Journal Article
Cross, W., Lyskjær, I., Lesluyes, T., Hargreaves, S., Strobl, A.-C., Davies, C., Waise, S., Hames-Fathi, S., Oukrif, D., Ye, H., Amary, F., Tirabosco, R., Gerrand, C., Baker, T., Barnes, D., Steele, C., Alexandrov, L., Bond, G., Cool, P., Pillay, N., …Research Consortium, G. E. (2022). A genetic model for central chondrosarcoma evolution correlates with patient outcome. Genome Medicine, 14(99), https://doi.org/10.1186/s13073-022-01084-0

BACKGROUND: Central conventional chondrosarcoma (CS) is the most common subtype of primary malignant bone tumour in adults. Treatment options are usually limited to surgery, and prognosis is challenging. These tumours are characterised by the presenc... Read More about A genetic model for central chondrosarcoma evolution correlates with patient outcome.

Image Guided Needle Biopsies for Musculoskeletal Soft Tissue Lesions: A 9-Year Analysis of Non-Diagnostic Biopsies (2022)
Journal Article
Morris, R., Winn, N., Singh, J., Tyrrell, P., Kaur, S., Amary, F., & Cool, P. (2022). Image Guided Needle Biopsies for Musculoskeletal Soft Tissue Lesions: A 9-Year Analysis of Non-Diagnostic Biopsies. Journal of Orthopaedic Oncology, 8(3), Article 168. https://doi.org/10.4172/2472-016X.1000168

Background: Non-diagnostic (ND) musculoskeletal biopsies of soft tissue lesions can increase patient anxiety and delay definitive diagnosis and treatment. It is thought the specific histotype of the lesion may influence accuracy of the biopsy. This s... Read More about Image Guided Needle Biopsies for Musculoskeletal Soft Tissue Lesions: A 9-Year Analysis of Non-Diagnostic Biopsies.

Mirels’ score for upper limb metastatic lesions: do we need a different cutoff for recommending prophylactic fixation? (2022)
Journal Article
Hoban, K. A., Downie, S., Adamson, D. J., MacLean, J. G., Cool, P., & Jariwala, A. C. (2022). Mirels’ score for upper limb metastatic lesions: do we need a different cutoff for recommending prophylactic fixation?. https://doi.org/10.1016/j.jseint.2022.03.006

The aim of this study was to investigate the reproducibility, reliability and accuracy of Mirels’s score in upper limb bony metastatic disease and validate its use in predicting pathological fractures. Methods 45 patients with upper limb bony metasta... Read More about Mirels’ score for upper limb metastatic lesions: do we need a different cutoff for recommending prophylactic fixation?.