Outputs (37)

Fourier Transform Infrared microspectroscopy identifies single cancer cells in blood. A feasibility study towards liquid biopsy (2023)
Journal Article

The management of cancer patients has markedly improved with the advent of personalised medicine where treatments are given based on tumour antigen expression amongst other. Within this remit, liquid biopsies will no doubt improve this personalised c... Read More about Fourier Transform Infrared microspectroscopy identifies single cancer cells in blood. A feasibility study towards liquid biopsy.

Fourier transform infrared spectroscopy as a non-destructive method for analysing herbarium specimens. (2023)
Journal Article

Dried plant specimens stored in herbaria are an untapped treasure chest of information on environmental conditions, plant evolution and change over many hundreds of years. Owing to their delicate nature and irreplaceability, there is limited access f... Read More about Fourier transform infrared spectroscopy as a non-destructive method for analysing herbarium specimens..

Domes and semi-capsules as model systems for infrared microspectroscopy of biological cells. (2023)
Journal Article

It is well known that infrared microscopy of micrometer sized samples suffers from strong scattering distortions, attributed to Mie scattering. The state-of-the-art preprocessing technique for modelling and removing Mie scattering features from infra... Read More about Domes and semi-capsules as model systems for infrared microspectroscopy of biological cells..

Detection of lipid efflux from foam cell models using a label-free infrared method (2022)
Journal Article

Cardiovascular diseases are still among the leading causes of mortality and morbidity worldwide. The build-up of fatty plaques in the arteries, leading to atherosclerosis, is the most common cause of cardiovascular diseases. The central player in ath... Read More about Detection of lipid efflux from foam cell models using a label-free infrared method.

Physiological Oxygen Causes the Release of Volatile Organic Compounds from Human Pluripotent Stem Cells with Possible Roles in Maintaining Self-Renewal and Pluripotency (2022)
Journal Article

Human pluripotent stem cells (hPSCs) have widespread potential biomedical applications. There is a need for large-scale in vitro production of hPSCs, and optimal culture methods are vital in achieving this. Physiological oxygen (2% O2) improves key h... Read More about Physiological Oxygen Causes the Release of Volatile Organic Compounds from Human Pluripotent Stem Cells with Possible Roles in Maintaining Self-Renewal and Pluripotency.

Clinical applications of infrared and Raman spectroscopy in the fields of cancer and infectious diseases (2021)
Journal Article

Analytical technologies that can improve disease diagnosis are highly sought after. Current screening/diagnostic tests for several diseases are limited by their moderate diagnostic performance, invasiveness, costly and laborious methodologies or the... Read More about Clinical applications of infrared and Raman spectroscopy in the fields of cancer and infectious diseases.

Optical Photothermal Infrared Microspectroscopy Discriminates for the First Time Different Types of Lung Cells on Histopathology Glass Slides. (2021)
Journal Article

The debate of whether a glass substrate can be used in Fourier transform infrared spectroscopy is strongly linked to its potential clinical application. Histopathology glass slides of 1 mm thickness absorb the mid-IR spectrum in the rich fingerprint... Read More about Optical Photothermal Infrared Microspectroscopy Discriminates for the First Time Different Types of Lung Cells on Histopathology Glass Slides..

Virtual Imaging for Patient Information on Radiotherapy Planning and Delivery for Prostate Cancer (2021)
Book Chapter

The provision of information on radiotherapy (RT) planning and delivery for patient with cancer is a vital issue in improving not only patients’ satisfaction but also patients’ compliance to treatment. In the present study, patients with prostate can... Read More about Virtual Imaging for Patient Information on Radiotherapy Planning and Delivery for Prostate Cancer.

Optimisation of sample preparation using glass slides for spectral pathology (2020)
Journal Article

The clinical translation of Fourier Transform Infrared (FTIR) microspectroscopy in pathology will require bringing this technique as close as possible to standard practice in pathology departments. An important step is sample preparation for both FTI... Read More about Optimisation of sample preparation using glass slides for spectral pathology.

Identification of a Glass Substrate to Study Cells Using Fourier Transform Infrared Spectroscopy: Are We Closer to Spectral Pathology? (2019)
Journal Article

The rising incidence of cancer worldwide is causing an increase in the workload in pathology departments. This, coupled with advanced analysis methodologies, supports a developing need for techniques that could identify the presence of cancer cells i... Read More about Identification of a Glass Substrate to Study Cells Using Fourier Transform Infrared Spectroscopy: Are We Closer to Spectral Pathology?.

Striking lung cancer response to self-administration of cannabidiol: a case report and literature review (2019)
Journal Article

In spite of new drugs, lung cancer is associated with a very poor prognosis. Whilst targeted therapies are improving outcomes, it is not uncommon for many patients to have only a partial response, and relapse during follow up. Thus, new drugs or re-e... Read More about Striking lung cancer response to self-administration of cannabidiol: a case report and literature review.

Fourier transform infrared spectra of cells on glass coverslips: a further step in spectral pathology (2018)
Journal Article

Over the last few years, great effort has been placed on developing Fourier Transform Infrared (FTIR) microspectroscopy as a tool to help in the histopathological diagnosis of cancer. The ever increasing workload in pathology departments is calling f... Read More about Fourier transform infrared spectra of cells on glass coverslips: a further step in spectral pathology.

Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model. (2018)
Journal Article

Isolated and monolayer expanded chondrocytes are not the ideal cell form to produce a cartilage matrix. In articular cartilage, each chondrocyte is surrounded by a 2-4 µm thick collagen VI-rich pericellular matrix (PCM) forming a chondron. Freshly ex... Read More about Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model..

Clinical applications of infrared and Raman spectroscopy: state of play and future challenges. (2018)
Journal Article

Vibrational spectroscopies, based on infrared absorption and/or Raman scattering provide a detailed fingerprint of a material, based on the chemical content. Diagnostic and prognostic tools based on these technologies have the potential to revolution... Read More about Clinical applications of infrared and Raman spectroscopy: state of play and future challenges..

Correction: Clinical applications of infrared and Raman spectroscopy: state of play and future challenges. (2018)
Journal Article

Correction for 'Clinical applications of infrared and Raman spectroscopy: state of play and future challenges' by Matthew J. Baker, et al., Analyst, 2018, DOI: 10.1039/c7an01871a.

Artificial Neural Network Analysis of Volatile Organic Compounds for the detection of lung cancer (2017)
Conference Proceeding

Lung cancer is a widespread disease and it is well understood that systematic, non-invasive and early detection of this progressive and life-threatening disorder is of vital importance for patient outcomes. In this work we present a convergence of fa... Read More about Artificial Neural Network Analysis of Volatile Organic Compounds for the detection of lung cancer.

Evaluation of peroxidative stress of cancer cells in vitro by real-time quantification of volatile aldehydes in culture headspace (2017)
Journal Article

Rationale Peroxidation of lipids in cellular membranes results in the release of volatile organic compounds (VOCs), including saturated aldehydes. The real-time quantification of trace VOCs produced by cancer cells during peroxidative stress present... Read More about Evaluation of peroxidative stress of cancer cells in vitro by real-time quantification of volatile aldehydes in culture headspace.

Effects of nilotinib on leukaemia cells using vibrational microspectroscopy and cell cloning (2016)
Journal Article

Over the last few years, both synchrotron-based FTIR (S-FTIR) and Raman microspectroscopies have helped to better understand the effects of drugs on cancer cells. However, cancer is a mixture of cells with different sensitivity/resistance to drugs. F... Read More about Effects of nilotinib on leukaemia cells using vibrational microspectroscopy and cell cloning.

Clinical Spectroscopy: general discussion (2016)
Journal Article

Biofluids and other techniques: general discussion (2016)
Journal Article

Single cell analysis/data handling: general discussion (2016)
Journal Article

Virtual imaging for patient information on radiotherapy planning and delivery (2016)
Presentation / Conference

Purpose or Objective: To assess whether both patients and their relatives would welcome further information on a oneto-one basis on RT planning and delivery using the virtual reality (VR) system VERT.

Infrared study of the biochemical effects in glioma cells induced by x-rays and Gd nanoparticles: first studies at SESAME synchrotron (Jordan) (2016)
Journal Article

Precision control of dissolved oxygen in mammalian cell culture media impacts on in situ volatile generation and promotes improved mesenchymal stem cell yield accompanied by reduced transcriptional variability (2015)
Journal Article

Mammalian cell culture is reliant on culture media to support in vitro growth and proliferation. Culture media contains salts, vitamins, and minerals at precise levels where subtle variations result in novel media formulations. Oxygen is a bioactive... Read More about Precision control of dissolved oxygen in mammalian cell culture media impacts on in situ volatile generation and promotes improved mesenchymal stem cell yield accompanied by reduced transcriptional variability.

Pilot study on virtual imaging for patient information on radiotherapy planning and delivery (2015)
Journal Article

It is widely accepted that health professionals might sometimes underestimate cancer patients' needs for information on the complex process of radiotherapy (RT) planning and delivery. Furthermore, relatives might also feel excluded from the treatment... Read More about Pilot study on virtual imaging for patient information on radiotherapy planning and delivery.

Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy (2014)
Journal Article

We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analy... Read More about Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy.

Using Fourier transform IR spectroscopy to analyze biological materials (2014)
Journal Article

IR spectroscopy is an excellent method for biological analyses. It enables the nonperturbative, label-free extraction of biochemical information and images toward diagnosis and the assessment of cell functionality. Although not strictly microscopy in... Read More about Using Fourier transform IR spectroscopy to analyze biological materials.

Study of gemcitabine-sensitive/resistant cancer cells by cell cloning and synchrotron FTIR microspectroscopy (2014)
Journal Article

Over the last few years, significant scientific insight on the effects of chemotherapy drugs at cellular level using synchrotron-based FTIR (S-FTIR) microspectroscopy has been obtained. The work carried out so far has identified spectral differences... Read More about Study of gemcitabine-sensitive/resistant cancer cells by cell cloning and synchrotron FTIR microspectroscopy.

Study of the biochemical effects induced by X-ray irradiations in combination with gadolinium nanoparticles in F98 glioma cells: first FTIR studies at the Emira laboratory of the SESAME synchrotron (2014)
Journal Article

Over the last few years, significant scientific insight on the effects of chemotherapy drugs at cellular level using synchrotron-based FTIR (S-FTIR) microspectroscopy has been obtained. The work carried out so far has identified spectral differences... Read More about Study of the biochemical effects induced by X-ray irradiations in combination with gadolinium nanoparticles in F98 glioma cells: first FTIR studies at the Emira laboratory of the SESAME synchrotron.

Vibrational spectroscopy in stem cell characterisation: is there a niche? (2014)
Journal Article

Vibrational spectroscopy using both infrared and Raman spectroscopies has been used in recent years with the aim to aid clinicians in disease diagnosis. More recently, these techniques have been applied to study stem cell differentiation and to deter... Read More about Vibrational spectroscopy in stem cell characterisation: is there a niche?.

On evaluation of a multiscale-based CT image analysis and visualisation algorithm (2013)
Conference Proceeding

Development of computed tomography (CT) protocols that minimise radiation dose for specific clinical treatments continues to be a major research focus. Building on the success of an earlier collaborative case study concerning prostate cancer diagnosi... Read More about On evaluation of a multiscale-based CT image analysis and visualisation algorithm.

A microscopic and macroscopic study of aging collagen on its molecular structure, mechanical properties, and cellular response (2013)
Journal Article

During aging, collagen structure changes, detrimentally affecting tissues' biophysical and biomechanical properties due to an accumulation of advanced glycation end-products (AGEs). In this investigation, we conducted a parallel study of microscopic... Read More about A microscopic and macroscopic study of aging collagen on its molecular structure, mechanical properties, and cellular response.

Anti-cancer thymoquinone from Nigella sativa (2013)
Journal Article

Quinones and phenolic compounds are important bioactive natural products in medicinal and dietary plants [1 – 3]. For example, thymoquinone (2-isopropyl-6-methyl-1,4-benzoquinone, TQ) is the main bioactive compound from Nigella sativa (black cumin) (... Read More about Anti-cancer thymoquinone from Nigella sativa.

Identification of different subsets of lung cells using Raman microspectroscopy and whole cell nucleus isolation (2013)
Journal Article

Raman spectroscopy has been widely used to study its possible clinical application in cancer diagnosis. However, in order to make it into clinical practice, it is important that this technique is able not only to identify cancer cells from their norm... Read More about Identification of different subsets of lung cells using Raman microspectroscopy and whole cell nucleus isolation.

Quantification by SIFT-MS of acetaldehyde released by lung cells in a 3D model (2012)
Journal Article

Our previous studies have shown that both lung cancer cells and non-malignant lung cells release acetaldehyde in vitro. However, data from other laboratories have produced conflicting results. Furthermore, all these studies have been carried out in 2... Read More about Quantification by SIFT-MS of acetaldehyde released by lung cells in a 3D model.

Vibrational spectroscopy differentiates between multipotent and pluripotent stem cells (2010)
Journal Article

Spectropathology for the next generation: Quo vadis?
Journal Article

Although the potential of vibrational spectroscopy for biomedical applications has been well demonstrated, translation into clinical practice has been relatively slow. This Editorial assesses the challenges facing the field and the potential way forw... Read More about Spectropathology for the next generation: Quo vadis?.