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Outputs (114)

Optical Microscopy Using the Faraday Effect Reveals in Situ Magnetization Dynamics of Magnetic Nanoparticles in Biological Samples (2024)
Journal Article

The study of exogenous and endogenous nanoscale magnetic material in biology is important for developing biomedical nanotechnology as well as for understanding fundamental biological processes such as iron metabolism and biomineralization. Here, we e... Read More about Optical Microscopy Using the Faraday Effect Reveals in Situ Magnetization Dynamics of Magnetic Nanoparticles in Biological Samples.

Fabrication and Characterisation of Hydrogels with Reversible Wrinkled Surfaces for Limbal Study and Reconstruction (2023)
Journal Article

In the biomedical field, there is a demand for the development of novel approaches for the investigation of optical epithelial anatomical features with biomimetic materials. These materials are not only required to replicate structures but also enabl... Read More about Fabrication and Characterisation of Hydrogels with Reversible Wrinkled Surfaces for Limbal Study and Reconstruction.

Antifouling performance of D-enantiomers-based peptide-modified aluminum alloy surfaces with enhanced stability against proteolytic degradation (2023)
Journal Article

Marine biofouling presents a significant challenge to the sustainable development of the maritime industry. Antimicrobial peptide (AMP) offers a promising strategy to combat biofouling. However, the inherent susceptibility of natural AMPs composed of... Read More about Antifouling performance of D-enantiomers-based peptide-modified aluminum alloy surfaces with enhanced stability against proteolytic degradation.

The effect of distal aspiration catheter position on collateral flow in mechanical thrombectomy - an in vitro study (2023)
Journal Article

Endovascular mechanical thrombectomy for ischaemic stroke is one of the most effective treatments. Despite the devices and techniques that have been developed, thrombi are not always successfully retrieved. The incidence of futile reperfusion after s... Read More about The effect of distal aspiration catheter position on collateral flow in mechanical thrombectomy - an in vitro study.

Developing Biomimetic Hydrogels of the Arterial Wall as a Prothrombotic Substrate for In Vitro Human Thrombosis Models (2023)
Journal Article

Current in vitro thrombosis models utilise simplistic 2D surfaces coated with purified components of the subendothelial matrix. The lack of a realistic humanised model has led to greater study of thrombus formation in in vivo tests in animals. Here w... Read More about Developing Biomimetic Hydrogels of the Arterial Wall as a Prothrombotic Substrate for In Vitro Human Thrombosis Models.

Debiased ambient vibrations optical coherence elastography to profile cell, organoid and tissue mechanical properties (2023)
Journal Article

The role of the mechanical environment in defining tissue function, development and growth has been shown to be fundamental. Assessment of the changes in stiffness of tissue matrices at multiple scales has relied mostly on invasive and often speciali... Read More about Debiased ambient vibrations optical coherence elastography to profile cell, organoid and tissue mechanical properties.

INVESTIGATION OF CELL AGGREGATE SIZE AND POTENTIAL BONE GROWTH WITH BONE CELL LINE AND HUMAN MESENCHYMAL STEM CELLS (2023)
Conference Proceeding

Our previous research has demonstrated that minor adjustments to in vitro cellular aggregation parameters, i.e. alterations to aggregate size, can influence temporal and spatial mineral depositions within maturing bone cell nodules. What remains uncl... Read More about INVESTIGATION OF CELL AGGREGATE SIZE AND POTENTIAL BONE GROWTH WITH BONE CELL LINE AND HUMAN MESENCHYMAL STEM CELLS.

Development of Boron-Containing PVA-Based Cryogels with Controllable Boron Releasing Rate and Altered Influence on Osteoblasts (2023)
Journal Article

Cryogel formation is an effective approach to produce porous scaffolds for tissue engineering. In this study, cryogelation was performed to produce boron-containing scaffolds for bone tissue engineering. A combination of the synthetic polymer, poly(v... Read More about Development of Boron-Containing PVA-Based Cryogels with Controllable Boron Releasing Rate and Altered Influence on Osteoblasts.

Hydrostatic pressure stimulates the osteogenesis and angiogenesis of MSCs/HUVECs co-culture on porous PLGA scaffolds (2022)
Journal Article

In native bone tissue regeneration, blood vessels, providing oxygen and nutrition for tissues, can promote the regeneration of bone and accelerate the repair of a defected area. In this study, Poly(D, L-lactic-co-glycolic acid) (PLGA) inverse opal sc... Read More about Hydrostatic pressure stimulates the osteogenesis and angiogenesis of MSCs/HUVECs co-culture on porous PLGA scaffolds.

In Vivo Antibacterial Efficacy of Antimicrobial Peptides Modified Metallic Implants-Systematic Review and Meta-Analysis. (2022)
Journal Article

Biomaterial-associated infection is difficult to detect and brings consequences that can lead to morbidity and mortality. Bacteria can adhere to the implant surface, grow, and form biofilms. Antimicrobial peptides (AMPs) can target and kill bacterial... Read More about In Vivo Antibacterial Efficacy of Antimicrobial Peptides Modified Metallic Implants-Systematic Review and Meta-Analysis..

New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties. (2022)
Journal Article

Preterm prelabour rupture of membranes is the leading cause of preterm birth and its associated infant mortality and morbidity. However, its underlying mechanism remains unknown. We utilized two novel biomechanical assessment techniques, ball indenta... Read More about New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties..

New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties (2022)
Journal Article

Preterm prelabour rupture of membranes is the leading cause of preterm birth and its associated infant mortality and morbidity. However, its underlying mechanism remains unknown. We utilized two novel biomechanical assessment techniques, ball indenta... Read More about New approaches suggest term and preterm human fetal membranes may have distinct biomechanical properties.

The Combination of Tissue-Engineered Blood Vessel Constructs and Parallel Flow Chamber Provides a Potential Alternative to In Vivo Drug Testing Models (2021)
Journal Article

Cardiovascular disease is a major cause of death globally. This has led to significant efforts to develop new anti-thrombotic therapies or re-purpose existing drugs to treat cardiovascular diseases. Due to difficulties of obtaining healthy human bloo... Read More about The Combination of Tissue-Engineered Blood Vessel Constructs and Parallel Flow Chamber Provides a Potential Alternative to In Vivo Drug Testing Models.

Quantitative measurement of mechanical properties in wound healing processes in a corneal stroma model by using vibrational optical coherence elastography (OCE) (2021)
Journal Article

Corneal wound healing, caused by frequent traumatic injury to the cornea and increasing numbers of refractive surgeries, has become a vital clinical problem. In the cornea, wound healing is an extremely complicated process. However, little is known a... Read More about Quantitative measurement of mechanical properties in wound healing processes in a corneal stroma model by using vibrational optical coherence elastography (OCE).

Dose-response association between physical activity and clustering of modifiable cardiovascular risk factors among 26,093 Chinese adults (2020)
Journal Article

Background There is uncertain evidence in the dose-response association between overall physical activity levels and clustering of cardiovascular diseases modifiable risk factors (CVDMRF) in Chinese adults. This study examined the hypothesis whether... Read More about Dose-response association between physical activity and clustering of modifiable cardiovascular risk factors among 26,093 Chinese adults.

Synthesis of magnetic nanoparticles with an IDA or TED modified surface for purification and immobilization of poly-histidine tagged proteins (2020)
Journal Article

Magnetic nanoparticles (MNPs) chelating with metal ions can specifically interact with poly-histidine peptides and facilitate immobilization and purification of proteins with poly-histidine tags. Fabrication of MNPs is generally complicated and time... Read More about Synthesis of magnetic nanoparticles with an IDA or TED modified surface for purification and immobilization of poly-histidine tagged proteins.

Development and evaluation of cationic nanostructured lipid carriers for ophthalmic drug delivery of besifloxacin (2019)
Journal Article

Besifloxacin hydrochloride (BSF) is a new ophthalmic antibiotic. However, its low water solubility limits its therapeutic efficacy. This article reported a novel lipid based drug delivery system to enhance ocular bioavailability of BSF. Cationic nano... Read More about Development and evaluation of cationic nanostructured lipid carriers for ophthalmic drug delivery of besifloxacin.

Investigation of Conjunctival Fibrosis Response Using a 3D Glaucoma Tenon’s Capsule + Conjunctival ModelFibrosis Response Within a 3D Tenon’s + Conjunctival Model (2019)
Journal Article

Surgical techniques such as trabeculectomy aim to treat glaucoma by making an incision into the scleral tissue, to create an alternative drainage pathway for aqueous to flow into the sub-Tenon’s/subconjunctival space. However, tissue fibrosis and wou... Read More about Investigation of Conjunctival Fibrosis Response Using a 3D Glaucoma Tenon’s Capsule + Conjunctival ModelFibrosis Response Within a 3D Tenon’s + Conjunctival Model.

Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model. (2018)
Journal Article

Isolated and monolayer expanded chondrocytes are not the ideal cell form to produce a cartilage matrix. In articular cartilage, each chondrocyte is surrounded by a 2-4 µm thick collagen VI-rich pericellular matrix (PCM) forming a chondron. Freshly ex... Read More about Vibrational spectroscopic monitoring and biochemical analysis of pericellular matrix formation and maturation in a 3-dimensional chondrocyte culture model..

Induction of zonal-specific cellular morphology and matrix synthesis for biomimetic cartilage regeneration using hybrid scaffolds. (2018)
Journal Article

Cartilage is anisotropic in nature and organized into distinct zones. Our goal was to develop zonal-specific three-dimensional hybrid scaffolds which could induce the generation of zonal-specific cellular morphology and extracellular matrix (ECM) com... Read More about Induction of zonal-specific cellular morphology and matrix synthesis for biomimetic cartilage regeneration using hybrid scaffolds..

The Effects of Biomimetically Conjugated VEGF on Osteogenesis and Angiogenesis of MSCs (Human and Rat) and HUVECs Co-culture Models (2018)
Journal Article

The purpose of this work was to investigate if the biomimetically conjugated VEGF and HUVECs co-culture could modulate the osteogenic and angiogenic differentiation of MSCs derived from rat and human bone marrow (rMSCs and hMSCs). After treated by am... Read More about The Effects of Biomimetically Conjugated VEGF on Osteogenesis and Angiogenesis of MSCs (Human and Rat) and HUVECs Co-culture Models.

Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy (2018)
Journal Article

Cell mechanical behaviour is increasingly recognised as a central biophysical parameter in cancer and stem cell research, and methods of investigating their mechanical behaviour are therefore needed. We have developed a novel qualitative method based... Read More about Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy.

Co-culture of chondrons and mesenchymal stromal cells reduces the loss of collagen VI and improves extracellular matrix production. (2017)
Journal Article

Adult articular chondrocytes are surrounded by a pericellular matrix (PCM) to form a chondron. The PCM is rich in hyaluronan, proteoglycans, and collagen II, and it is the exclusive location of collagen VI in articular cartilage. Collagen VI anchors... Read More about Co-culture of chondrons and mesenchymal stromal cells reduces the loss of collagen VI and improves extracellular matrix production..

Co-culture of chondrons and mesenchymal stromal cells reduces the loss of collagen VI and improves extracellular matrix production (2017)
Journal Article

Adult articular chondrocytes are surrounded by a pericellular matrix (PCM) to form a chondron. The PCM is rich in hyaluronan, proteoglycans, and collagen II, and it is the exclusive location of collagen VI in articular cartilage. Collagen VI anchors... Read More about Co-culture of chondrons and mesenchymal stromal cells reduces the loss of collagen VI and improves extracellular matrix production.

Fluorescent Labeling of Collagen Production by Cells for Noninvasive Imaging of Extracellular Matrix Deposition. (2017)
Journal Article

Extracellular matrix (ECM) is an essential component of tissues and provides both integrity and biological cues for cells. Collagen is one of the major proteins found within the ECM and therefore is an essential component of all engineered tissues. T... Read More about Fluorescent Labeling of Collagen Production by Cells for Noninvasive Imaging of Extracellular Matrix Deposition..

Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy (2017)
Journal Article

There is an unmet need in tissue engineering for non-invasive, label-free monitoring of cell mechanical behaviour in their physiological environment. Here, we describe a novel optical coherence phase microscopy (OCPM) set-up which can map relative... Read More about Real-time and non-invasive measurements of cell mechanical behaviour with optical coherence phase microscopy.

Influence of Additive Manufactured Scaffold Architecture on the Distribution of Surface Strains and Fluid Flow Shear Stresses and Expected Osteochondral Cell Differentiation (2017)
Journal Article

Scaffolds for regenerative medicine applications should instruct cells with the appropriate signals, including biophysical stimuli such as stress and strain, to form the desired tissue. Apart from that, scaffolds, especially for load-bearing applicat... Read More about Influence of Additive Manufactured Scaffold Architecture on the Distribution of Surface Strains and Fluid Flow Shear Stresses and Expected Osteochondral Cell Differentiation.

Online Monitoring Of Mechanical Properties Of 3D Tissue Engineered Constructs For Quality Assessment (2016)
Conference Proceeding

Introduction: Monitoring the maturation of engineered tissues prior to implantation into the patient is important for defining quality and manufacturing criteria. Key properties include scaffold integrity and mechanical properties. Novel non-invasive... Read More about Online Monitoring Of Mechanical Properties Of 3D Tissue Engineered Constructs For Quality Assessment.

Defining a turnover index for the correlation of biomaterial degradation and cell based extracellular matrix synthesis using fluorescent tagging techniques (2016)
Journal Article

Non-destructive protocols which can define a biomaterial’s degradation and its associated ability to support proliferation and/or promote extracellular matrix deposition will be an essential in vitro tool. In this study we investigate fluorescently t... Read More about Defining a turnover index for the correlation of biomaterial degradation and cell based extracellular matrix synthesis using fluorescent tagging techniques.

Comparability: manufacturing, characterization and controls, report of a UK Regenerative Medicine Platform Pluripotent Stem Cell Platform Workshop, Trinity Hall, Cambridge, 14-15 September 2015. (2016)
Journal Article

This paper summarizes the proceedings of a workshop held at Trinity Hall, Cambridge to discuss comparability and includes additional information and references to related information added subsequently to the workshop. Comparability is the need to de... Read More about Comparability: manufacturing, characterization and controls, report of a UK Regenerative Medicine Platform Pluripotent Stem Cell Platform Workshop, Trinity Hall, Cambridge, 14-15 September 2015..

A Real-Time Monitoring System to Assess the Platelet Aggregatory Capacity of Components of a Tissue-Engineered Blood Vessel Wall. (2016)
Journal Article

Native blood vessels contain both an antiaggregatory intimal layer, which prevents platelet activation in the intact vessel, and a proaggregatory medial layer, which stimulates platelet aggregation upon vascular damage. Yet, current techniques for as... Read More about A Real-Time Monitoring System to Assess the Platelet Aggregatory Capacity of Components of a Tissue-Engineered Blood Vessel Wall..

Accelerating and increasing nano-scaled pore formation on electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fibers. (2016)
Journal Article

Porous fibers are advantageous for filtration systems, drug delivery systems, and in the field of tissue engineering, in comparison to their non-porous counterparts. In this study, we developed a facile technique including two steps to generate poly(... Read More about Accelerating and increasing nano-scaled pore formation on electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) fibers..

Nanofibrous scaffolds supporting optimal central nervous system regeneration: an evidence-based review (2015)
Journal Article

Restoration of function following damage to the central nervous system (CNS) is severely restricted by several factors. These include the hindrance of axonal regeneration imposed by glial scars resulting from inflammatory response to damage, and limi... Read More about Nanofibrous scaffolds supporting optimal central nervous system regeneration: an evidence-based review.

Nano-colloid printing of functionalised PLA-b-PEO copolymers: Tailoring the surface pattern of adhesive motif and its effect on cell attachment (2015)
Journal Article

n this study, we investigate the preparation of surface pattern of functional groups on poly(lactide) (PLA) surfaces through the controlled deposition of core-shell self-assemblies based on functionalized PLA-b-PEO amphiphilic block copolymers from s... Read More about Nano-colloid printing of functionalised PLA-b-PEO copolymers: Tailoring the surface pattern of adhesive motif and its effect on cell attachment.

Fluorescent Monitoring of Poly(lactic-co-glycolic Acid) Degradation and Extracellular Matrix Turnover of Engineered Tissue Constructs (2015)
Presentation / Conference

Development of fluorescent tagging techniques for inert polymers is an advantageous tool for monitoring biomaterial degradation online. Here we present a protocol for the fluorescent tagging of poly(lactic-co-glycolic acid) (PLGA) which allows for ac... Read More about Fluorescent Monitoring of Poly(lactic-co-glycolic Acid) Degradation and Extracellular Matrix Turnover of Engineered Tissue Constructs.

In vitro experiments of cerebral blood flow during aspiration thrombectomy: potential effects on cerebral perfusion pressure and collateral flow (2015)
Journal Article

BACKGROUND: Mechanical thrombectomy with stent retriever devices is associated with significantly better outcomes than thrombolysis alone in the treatment of acute ischemic stroke. Thrombus aspiration achieves high patency rates, but clinical outcome... Read More about In vitro experiments of cerebral blood flow during aspiration thrombectomy: potential effects on cerebral perfusion pressure and collateral flow.

Application of new optical coherence elastography to monitor the mineralization processing in bone tissue engineering constructs (2015)
Journal Article

Generation of functional tissue in vitro through tissue engineering technique is a promising direction to repair and replace malfunctioned organ and tissue in the modern medicine for various diseases which could not been treated well by conventional... Read More about Application of new optical coherence elastography to monitor the mineralization processing in bone tissue engineering constructs.

Efficient alignment of primary CNS neurites using structurally engineered surfaces and biochemical cues (2015)
Journal Article

Tissue engineering strategies for the central nervous system (CNS) have been largely hampered by the complexity of neural cell interactions and limited ability to control functional circuit formation. Although cultures of primary CNS neurons give key... Read More about Efficient alignment of primary CNS neurites using structurally engineered surfaces and biochemical cues.

Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy (2014)
Journal Article

We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analy... Read More about Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy.

Study of gemcitabine-sensitive/resistant cancer cells by cell cloning and synchrotron FTIR microspectroscopy (2014)
Journal Article

Over the last few years, significant scientific insight on the effects of chemotherapy drugs at cellular level using synchrotron-based FTIR (S-FTIR) microspectroscopy has been obtained. The work carried out so far has identified spectral differences... Read More about Study of gemcitabine-sensitive/resistant cancer cells by cell cloning and synchrotron FTIR microspectroscopy.

Development of a stereotaxic device for low impact implantation of neural constructs or pieces of neural tissues into the mammalian brain (2014)
Journal Article

Implanting pieces of tissue or scaffolding material into the mammalian central nervous system (CNS) is wrought with difficulties surrounding the size of tools needed to conduct such implants and the ability to maintain the orientation and integrity o... Read More about Development of a stereotaxic device for low impact implantation of neural constructs or pieces of neural tissues into the mammalian brain.

Quantitative evaluation of degenerated tendon model using combined optical coherence elastography and acoustic radiation force method (2013)
Journal Article

Damage of collagen fibers in tendons is often directly related to changes in a tendon’s mechanical properties. Direct quantitative elasticity measurement of tendons will provide important information in tendon dysfunction diagnosis and treatment asse... Read More about Quantitative evaluation of degenerated tendon model using combined optical coherence elastography and acoustic radiation force method.

Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury (2013)
Journal Article

Non-neuronal cells of the central nervous system (CNS), termed “neuroglia,” play critical roles in neural regeneration; therefore, replacement of glial populations via implantable nanofabricated devices (providing a growth-permissive niche) is a prom... Read More about Alignment of multiple glial cell populations in 3D nanofiber scaffolds: Toward the development of multicellular implantable scaffolds for repair of neural injury.

A microscopic and macroscopic study of aging collagen on its molecular structure, mechanical properties, and cellular response (2013)
Journal Article

During aging, collagen structure changes, detrimentally affecting tissues' biophysical and biomechanical properties due to an accumulation of advanced glycation end-products (AGEs). In this investigation, we conducted a parallel study of microscopic... Read More about A microscopic and macroscopic study of aging collagen on its molecular structure, mechanical properties, and cellular response.

Corneal Stromal Cell Plasticity: In Vitro Regulation of Cell Phenotype Through Cell-Cell Interactions in a Three-Dimensional Model (2013)
Journal Article

In vivo, epithelial cells are connected both anatomically and functionally with stromal keratocytes. Co-culturing aims at recapturing this cellular anatomy and functionality by bringing together two or more cell types within the same culture environm... Read More about Corneal Stromal Cell Plasticity: In Vitro Regulation of Cell Phenotype Through Cell-Cell Interactions in a Three-Dimensional Model.

Identification of different subsets of lung cells using Raman microspectroscopy and whole cell nucleus isolation (2013)
Journal Article

Raman spectroscopy has been widely used to study its possible clinical application in cancer diagnosis. However, in order to make it into clinical practice, it is important that this technique is able not only to identify cancer cells from their norm... Read More about Identification of different subsets of lung cells using Raman microspectroscopy and whole cell nucleus isolation.

Optical Coherence Tomography to delineate the interactions of PAMAM dendrimers with the porcine skin surface (2013)
Journal Article

Polyamidoamine (PAMAM) dendrimers have been topically applied to the skin and utilised as a permeation enhancer for a range of therapeutic compounds. However, very little is known about the mechanism of enhancement. This study used optical coherence... Read More about Optical Coherence Tomography to delineate the interactions of PAMAM dendrimers with the porcine skin surface.

Errata: Study of optical properties and proteoglycan content of tendons by polarization sensitive optical coherence tomography (2013)
Journal Article

This article [J. Biomed. Opt.. 17, (8 ), 081417 (2012)] was originally published online on 2 August 2012 with an error in the reference citation of Fig. 1. The correct figure caption and references for Fig. 1 are shown below. A new reference was adde... Read More about Errata: Study of optical properties and proteoglycan content of tendons by polarization sensitive optical coherence tomography.