MCZ Meneghetti
ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis
Meneghetti, MCZ; Deboni, P; Palomino, CMV; Braga, LP; Cavalheiro, RP; Viana, GM; Yates, EA; Nader, HB; Andrade De Lima, M
Authors
P Deboni
CMV Palomino
LP Braga
RP Cavalheiro
GM Viana
EA Yates
HB Nader
Marcelo Andrade De Lima m.andrade.de.lima@keele.ac.uk
Abstract
The cell surface and extracellular matrix polysaccharide, heparan sulfate (HS) conveys chemical information to control crucial biological processes. HS chains are synthesized in a non-template driven process mainly in the Golgi apparatus, involving a large number of enzymes capable of subtly modifying its substitution pattern, hence, its interactions and biological effects. Changes in the localization of HS-modifying enzymes throughout the Golgi were found to correlate with changes in the structure of HS, rather than protein expression levels. Following BFA treatment, the HS-modifying enzymes localized preferentially in COPII vesicles and at the trans-Golgi. Shortly after heparin treatment, the HS-modifying enzyme moved from cis to trans-Golgi, which coincided with increased HS sulfation. Finally, it was shown that COPI subunits and Sec24 gene expression changed. Collectively, these findings demonstrate that knowledge of the ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the complete delineation of HS biosynthesis.
Citation
Meneghetti, M., Deboni, P., Palomino, C., Braga, L., Cavalheiro, R., Viana, G., Yates, E., Nader, H., & Andrade De Lima, M. (2021). ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis. Carbohydrate Polymers, 117477--117477. https://doi.org/10.1016/j.carbpol.2020.117477
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 30, 2020 |
Publication Date | 2021-01 |
Publicly Available Date | May 26, 2023 |
Journal | Carbohydrate Polymers |
Print ISSN | 0144-8617 |
Publisher | Elsevier |
Pages | 117477--117477 |
DOI | https://doi.org/10.1016/j.carbpol.2020.117477 |
Keywords | Biosynthesis; Heparan sulfate; COPI; COPII; Golgi apparatus |
Public URL | https://keele-repository.worktribe.com/output/418625 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S0144861720316507?via\%3Dihub |
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Publisher Licence URL
https://creativecommons.org/licenses/by-nc-nd/4.0/
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