ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis

Meneghetti, MCZ; Deboni, P; Palomino, CMV; Braga, LP; Cavalheiro, RP; Viana, GM; Yates, EA; Nader, HB; Andrade De Lima, M

Abstract

The cell surface and extracellular matrix polysaccharide, heparan sulfate (HS) conveys chemical information to control crucial biological processes. HS chains are synthesized in a non-template driven process mainly in the Golgi apparatus, involving a large number of enzymes capable of subtly modifying its substitution pattern, hence, its interactions and biological effects. Changes in the localization of HS-modifying enzymes throughout the Golgi were found to correlate with changes in the structure of HS, rather than protein expression levels. Following BFA treatment, the HS-modifying enzymes localized preferentially in COPII vesicles and at the trans-Golgi. Shortly after heparin treatment, the HS-modifying enzyme moved from cis to trans-Golgi, which coincided with increased HS sulfation. Finally, it was shown that COPI subunits and Sec24 gene expression changed. Collectively, these findings demonstrate that knowledge of the ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the complete delineation of HS biosynthesis.

Citation

Meneghetti, M., Deboni, P., Palomino, C., Braga, L., Cavalheiro, R., Viana, G., …Andrade De Lima, M. (2021). ER-Golgi dynamics of HS-modifying enzymes via vesicular trafficking is a critical prerequisite for the delineation of HS biosynthesis. Carbohydrate Polymers, 117477--117477. https://doi.org/10.1016/j.carbpol.2020.117477

• Journal Article Type: Article
• Acceptance Date: Nov 30, 2020
• Publication Date: 2021-01
• Publicly Available Date: May 26, 2023
• Journal: Carbohydrate Polymers
• Print ISSN: 0144-8617
• Publisher: Elsevier
• Pages: 117477--117477
• DOI: https://doi.org/10.1016/j.carbpol.2020.117477
• Keywords: Biosynthesis; Heparan sulfate; COPI; COPII; Golgi apparatus
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0144861720316507?via\%3Dihub